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Improved amplification of fecal DNA supports non-invasive microsatellite genoty** of lesser long-nosed bats (Leptonycteris yerbabuenae)
Feces of animals that forage on nectar and fruit, including many species of bats, often contain DNA that is low in quality and quantity. We developed...
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Environmental effects on faecal genoty** success in mesocarnivores
Non-invasive genetic sampling can be used in research, monitoring, and conservation of wild animals to, for example, provide insights into diets,...
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DNA Amplification Using Promega’s PowerPlex® Fusion Systems (5C and 6C)
Multiplex amplification and ty** of short tandem repeat (STR) loci enable the rapid characterization of forensic samples for human identification...
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New insights into the DNA extraction and PCR amplification of minute ascomycetes in the genus Laboulbenia (Pezizomycotina, Laboulbeniales)
Molecular studies of fungi within the order Laboulbeniales ( Ascomycota , Pezizomycotina ) have been hampered for years because of their minute size,...
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IMPATIENT-qPCR: monitoring SELEX success during in vitro aptamer evolution
AbstractSELEX (Systematic Evolution of Ligands by Exponential enrichment) processes aim on the evolution of high-affinity aptamers as binding...
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Role of Extreme Climate Events in Amplification of Plant Invasion
Anthropogenic-induced changes in climate and its resultant extreme climatic events, such as changes in the annual cycles of precipitation and fire...
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Development of a Rapid Detection Method to Prorocentrum lima by Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
Prorocentrum lima , a widely distributed dinoflagellate known for its production of harmful biotoxins, poses a significant threat to humans,...
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Environmental field conditions and sampling effort affect the molecular identification success of livestock predators
For centuries, wolf depredation on livestock has triggered human–wildlife conflicts throughout Europe. Free-ranging dogs, however, are increasingly...
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Rapid screening of point mutations by mismatch amplification mutation assay PCR
AbstractMetabolic engineering frequently makes use of point mutation and saturation mutation library creation. At present, sequencing is the only...
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Versatility of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) from diagnosis of early pathological infection to mutation detection in organisms
Loop-mediated isothermal amplification (LAMP) is a rapid, state-of-the-art DNA amplification technology, used primarily for the quick diagnosis and...
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Native solitary bee reproductive success depends on early season precipitation and host plant richness
Spring-emerging bees depend upon the synchronized bloom times of angiosperms that provide pollen and nectar for offspring. The emergence of such bees...
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Laboratory validation of loop-mediated isothermal amplification (LAMP) assay for Limnoperna fortunei (Dunker 1857) detection
Among the invasive species known to occur in South America is the bivalve mollusc Limnoperna fortunei , whose presence is linked to several...
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Volatiles of symbiotic bacterial origin explain ectoparasitism and fledging success of hoopoes
BackgroundSome parasites use olfactory cues to detect their hosts and, since bacterial symbionts are partially responsible for animal odours, they...
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Genome-Wide Characterization of Nuclear Microsatellite Markers for Centranthus trinervis (= Valeriana trinervis) and Cross-Amplification in Five Congeneric Taxa
Centranthus is a small genus belonging to the family Caprifoliaceae, comprising approximately 16 taxa (nine species plus seven subspecies)....
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The development of a loop-mediated isothermal amplification (LAMP) assay to detect American foulbrood in managed honey bee populations
Paenibacillus larvae (American foulbrood) is a lethal and arguably the most destructive and economically important notifiable bacterial disease that...
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Fast detection of Citrus chlorotic dwarf-associated virus by loop-mediated isothermal amplification
Citrus chlorotic dwarf-associated virus (CCDaV) is among the most damaging pathogens that can cause significant reduction in both the yield and...
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Establishment success of alien Daphnia in the ancient Lake Biwa: insights from sedimentary archives
Invasive species pose serious threats to global biodiversity, prompting studies to identify factors underlying invasion and establishment success....
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MAF amplification licenses ERα through epigenetic remodelling to drive breast cancer metastasis
MAF amplification increases the risk of breast cancer (BCa) metastasis through mechanisms that are still poorly understood yet have important...
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Non-invasive measurements of respiration and heart rate across wildlife species using Eulerian Video Magnification of infrared thermal imagery
BackgroundAn animal’s metabolic rate, or energetic expenditure, both impacts and is impacted by interactions with its environment. However,...
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Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method
Enterococcus faecium is responsible for a highly contagious, drug-resistant nosocomial infection that often causes serious illness. In this study, a...
