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Protein–Protein Interaction: Tandem Affinity Purification in Bacteria
The discovery of protein–protein interaction networks can lead to the unveiling of protein complex(es) forming cellular machinerie(s) or reveal...
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Cation affinity purification of histidine-tagged proteins
AbstractProtein purification is a basic technology in both biological research and industrial production, and efficient, convenient, economical, and...
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An immobilized antibody-based affinity grid strategy for on-grid purification of target proteins enables high-resolution cryo-EM
In cryo-electron microscopy (cryo-EM), sample preparation poses a critical bottleneck, particularly for rare or fragile macromolecular assemblies and...
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Chromatographic purification of small extracellular vesicles using an affinity column for phospholipid membranes
ObjectivesThis study aimed to investigate whether chromatography using an ExoPUA column, an affinity column for phospholipid membranes, could...
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Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins
Proteins often interact with each other to form complexes and play functional roles in almost all cellular processes. The study of protein–protein...
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Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice Using Endogenous Streptavidin/FLAG Tags
Biological complexity is achieved through elaborate interactions between relatively few individual components. Affinity purification (AP) has allowed...
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Affinity Purification of Soluble and Membrane-Bound Protein Complexes by a FlpIn Strategy
For a long time, the isolation of native protein complexes from human cells was accomplished by immunoprecipitation experiments. However, success...
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Parallel Analysis of Protein–Protein and Protein–Metabolite Complexes Using a Single-Step Affinity Purification
Cellular proteinProteins–metaboliteMetabolites interactions (PMIProtein-metabolite interaction (PMI)), for decades relatively overlooked, are seeing...
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Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors
ObjectiveTo examine the influence of widely used protein affinity tags and the tobacco PR1a signal peptide (SP) on detection, purification and...
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Isolation of Plant Mitochondria Using Affinity Purification
Mitochondria are highly dynamic organelles that are vital for eukaryotic cellular metabolism. Mitochondria account only for a small fraction of the...
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Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs
β-barrel membrane proteins play a crucial role in bacterial pathogenesis and antibiotic resistance, making them a prime focus for the development of...
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Enzyme Purification
The growing importance of enzymes for the food, biotech, pharmaceutical, and cosmetics industries, among others, has increased the need for efficient...
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Protein–Protein Interaction Network Map** by Affinity Purification Cross-Linking Mass Spectrometry (AP-XL-MS) based Proteomics
Protein–protein interactions (PPIs) are the physical interactions formed among proteins. These interactions are primarily functional, i.e., they...
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MS2-MBP-Based Affinity Purification of Nucleus- or Cytoplasm-Localized lncRNA–Protein Complexes Formed In Vivo
With recent emergence of huge number of long noncoding RNAs (lncRNAs), purification of lncRNA–protein (lncRNP) complexes is fundamental to understand...
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Affinity Purification of Glycosylphosphatidylinositol-anchored Proteins by Alpha-Toxin
The glycosylphosphatidylinositol (GPI)-anchor modification attaches a lipid anchor to the C-terminus of a protein, tethering the protein to the cell...
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Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification
The interest in therapeutic monoclonal antibodies (mAbs) has significantly grown in the pharmaceutical industry, exceeding 100 FDA mAbs approved....
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Tandem Affinity Purification and Mass-Spectrometric Analysis of FACT and Associated Proteins
Isolation of a protein/complex is important for its biochemical and structural characterization with mechanistic insights. TAP (tandem affinity...
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Affinity Purification and Coimmunoprecipitation of Transenvelope Protein Complexes in Gram-Negative Bacteria
Multiprotein complexes are important machineries that organize a large number of different proteins into functional units. Studying protein-protein...
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Affinity-Purification Combined with Crosslinking Mass Spectrometry for Identification and Structural Modeling of Host–Pathogen Protein–Protein Complexes
Host–pathogen protein–protein interactions are highly complex and dynamic and mediate key steps in pathogen adhesion to host, host invasion, and...
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Affinity Purification-Mass Spectroscopy (AP-MS) and Co-Immunoprecipitation (Co-IP) Technique to Study Protein–Protein Interactions
Affinity purification-Mass spectroscopy (AP-MS) is a biochemical technique to identify the novel protein–protein interaction that occurs in the most...