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Isolation of Human Adipose-Derived Stromal/Stem Cells from Lipoaspirates
Adipose tissue is an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative...
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Combination of Adipose-Derived Stromal/Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications
Adipose-derived stromal/stem cells (ASCs) and decellularized adipose tissue (DAT) are adipose tissue products obtained from individuals undergoing...
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Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method
Adipose tissue provides a valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely...
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Adipose-derived stem cells (ASCs) culture in spinner flask: improving the parameters of culture in a microcarrier-based system
Prior to clinical use, extensive in vitro proliferation of human adipose-derived stem cells (ASCs) is required. Among the current options,...
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Isolation of Human Adipose-Derived Stem Cells
Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine....
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Photomodulation alleviates cellular senescence of aging adipose-derived stem cells
BackgroundMesenchymal stem cells (MSCs) therapies are emerging as a promising approach to therapeutic regeneration. Therapeutic persistence and...
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Anti-aging mechanism of different age donor-matched adipose-derived stem cells
BackgroundAdipose-derived stem cells (ASCs) have anti-aging and anti-obesity effects in aged animals, but the underlying molecular mechanism remains...
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Osteopontin Rejuvenates Senescent Adipose-Derived Stem Cells and Restores their Bone Tissue Regenerative Function
The regenerative function of stem cells is compromised when the proportion of senescent stem cells increases with ageing advance. Therefore,...
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Spheroid Formation of Human Adipose-Derived Stem Cells Using a Liquid Overlay Technique
Compared to two-dimensional monolayer culture, cells cultured in three-dimensional (3D) platforms provide a more biochemically and physiologically...
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Adipose-derived stem cells in immune-related skin disease: a review of current research and underlying mechanisms
Adipose-derived stem cells (ASCs) are a critical adult stem cell subpopulation and are widely utilized in the fields of regenerative medicine and...
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Analyses and Utilization of Selectively Tuned Human Adipose-Derived Stromal/Stem Cell Exosomes
We have developed a hollow fiber bioreactorBioreactor-based production system for manufacturing large quantities of extracellular vesicles (EVs)...
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Generating and Characterizing Adipose Spheroids from Adipose-Derived Stromal/Stem Cells
Advances in technology and automation over the past several decades have made it feasible to perform high-throughput compound screening with cell...
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Human platelet lysate stimulates neurotrophic properties of human adipose-derived stem cells better than Schwann cell-like cells
BackgroundTrauma-associated peripheral nerve injury is a widespread clinical problem causing sensory and motor disabilities. Schwann cells (SCs)...
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Isolation methods, proliferation, and adipogenic differentiation of adipose-derived stem cells from different fat depots in bovines
The adipose-derived stem cells (ASCs) are a valuable resource for regenerative medicine and essential materials for research in fat deposition....
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Low-glucose culture environment can enhance the wound healing capability of diabetic adipose-derived stem cells
BackgroundApplication of autologous adipose-derived stem cells (ASC) for diabetic chronic wounds has become an emerging treatment option. However,...
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Sex Hormone-Binding Globulin (SHBG) Maintains Proper Equine Adipose-Derived Stromal Cells (ASCs)’ Metabolic Functions and Negatively Regulates their Basal Adipogenic Potential
BackgroundSex hormone binding globulin (SHBG) deteriorated expression has been recently strongly correlated to increased level of circulating...
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Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic and Osteogenic Differentiation or Flow Cytometry-Based Analysis
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders....
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Comparison of therapeutic effects of mesenchymal stem cells derived from superficial and deep subcutaneous adipose tissues
BackgroundFibrosis is a common histological feature in the process from chronic organ injury to organ failure. Chronic tissue injury causes...
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Motion-Accommodating Dual-Layer Hydrogel Dressing to Deliver Adipose-Derived Stem Cells to Wounds
Background:Current dressing materials cannot secure a cell survival-promoting wound environment for stem cell delivery due to insufficient...
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Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation, Characterization, and Differentiation
Adult mesenchymal stromal/stem cells (MSCs) are a standard component of de novo tissue generation to treat and study injury, disease, and...