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Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity
Hydroxypyruvate reductase (HPR; EC 1.1.1.81) activity is integral to the photorespiratory pathway. Within photorespiration, HPR catalyzes the... -
Assay of Fe(III) Chelate Reductase Activity in Arabidopsis thaliana Root
A sensitive FerroZine assay is used to measure the membrane-bound ferric-chelate reductase activity in the Arabidopsis thaliana roots. In... -
A Redox-Regulated, Heterodimeric NADH:cinnamate Reductase in Vibrio ruber
AbstractGenes of putative reductases of α,β-unsaturated carboxylic acids are abundant among anaerobic and facultatively anaerobic microorganisms, yet...
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Nitrate Reductase Drives Nutrition Control and Disease Resistance in Tomato (Solanum lycopersicum L.) Cultivars
Nitrate reductase plays an important role in preventing the occurrence of diseases and insect pests, which could rapidly reduce the accumulation of...
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Acrylate Reductase of an Anaerobic Electron Transport Chain of the Marine Bacterium Shewanella woodyi
AbstractMany microorganisms are capable of anaerobic respiration in the absence of oxygen, by using different organic compounds as terminal acceptors...
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Hydroxy(phenyl)pyruvic acid reductase in Actaea racemosa L.: a putative enzyme in cimicifugic and fukinolic acid biosynthesis
Main conclusionHydroxy(phenyl)pyruvic acid reductase from Actaea racemosa catalyzes dual reactions in reducing 4-hydroxyphenylpyruvic acid as well...
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Methylenetetrahydrofolate Reductase Gene Polymorphism as a Risk Factor for Coronary Artery Disease
Hyperhomocysteinemia (HHcy) is one of the factors contributing to the pathogenesis of coronary artery disease (CAD). Besides nutritional deficiency...
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The critical role of a conserved lysine residue in periplasmic nitrate reductase catalyzed reactions
Periplasmic nitrate reductase NapA from Campylobacter jejuni ( C. jejuni ) contains a molybdenum cofactor (Moco) and a 4Fe–4S cluster and catalyzes the...
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Hysteretic response of N2O reductase activity to soil pH variations after application of lime to an acidic agricultural soil
N 2 O contributes to increasing the greenhouse effect and is also involved in stratospheric ozone depletion. In soil and water, N 2 O reductase catalyses...
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Comparison of Glutathione Reductase Gene Expression in Drought-Sensitive and Tolerant Soybean Lines Using Real-Time PCR
AbstractGlobal climate change and associated adverse abiotic stress conditions, such as drought, salinity, heavy metals, waterlogging, extreme...
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Engineering of an ene-reductase for producing the key intermediate of antiepileptic drug Brivaracetam
Abstract( R )-4-Propyldihydrofuran-2(3H)-one ( R -PDFO) is the key chiral intermediate for the antiepileptic drug Brivaracetam. Lacking a simple and...
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Structural and Functional Analyses of the Flavoprotein Disulfide Reductase FN0820 of Fusobacterium nucleatum
Escherichia coli RclA and Staphylococcus aureus MerA are part of the Group I flavoprotein disulfide reductase (FDR) family and have been implicated...
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Association Between Methylenetetrahydrofoate Reductase Gene C677T Polymorphism and Susceptibility to Polycystic Ovary Syndrome
Polycystic ovary syndrome (PCOS) is the most common form of endocrinopathy of women. Several studies have investigated the association of...
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γ-Aminobutyric acid boosts chilling tolerance by promoting the methionine sulfoxide reductase-thioredoxin reductase system in peach fruit
The effects of γ-aminobutyric acid (GABA) on promotion of chilling tolerance and the methionine sulfoxide reductase (MSR)-thioredoxin reductase...
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Repurposed inhibitor of bacterial dihydrodipicolinate reductase exhibits effective herbicidal activity
Herbicide resistance represents one of the biggest threats to our natural environment and agricultural sector. Thus, new herbicides are urgently...
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Development of transgenic mice overexpressing mouse carbonyl reductase 1
BackgroundCarbonyl reductase 1 (CBR1) is a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reductase with broad substrate specificity....
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Identification of a cinnamoyl-CoA reductase from Cinnamomum cassia involved in trans-cinnamaldehyde biosynthesis
Main conclusionThe identification of a functional cinnamoyl-CoA reductase enzyme from Cinnamomum cassia involved in trans- cinnamaldehyde...
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Ribonucleotide reductase subunit switching in hepatoblastoma drug response and relapse
Prognosis of children with high-risk hepatoblastoma (HB), the most common pediatric liver cancer, remains poor. In this study, we found...
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Identification and functional characterization of a novel aldo–keto reductase from Aloe vera
Main conclusionThe present investigation profoundly asserted the catalytic potential of plant-based aldo-ketoreductase, postulating its role in...
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A novel alkane monooxygenase evolved from a broken piece of ribonucleotide reductase in Geobacillus kaustophilus HTA426 isolated from Mariana Trench
We have accidentally found that a thermophilic Geobacillus kaustophilus HTA426 is capable of degrading alkanes although it has no alkane oxygenating...