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FLUTAX Staining of Cilliates
FLUTAX staining is an advanced technique for observing cortical microtubules of ciliates. This taxol derivative of dye can aid in the visualization...
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Hood and Shew Staining
Endophytic microorganisms are present in the healthy tissues of almost all plants. Generally, endophytes are detected through plant tissue...
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Supported Immunohistochemical Staining of Mucins
Immunohistochemistry (IHC) staining is the most common method to detect the distribution and localization of biomarkers in different parts of a...
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Improvement of gram staining effect by ethanol pretreatment and quantization of staining image by unsupervised machine learning
In this study, we propose an Ethanol Pretreatment Gram staining method that significantly enhances the color contrast of the stain, thereby improving...
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Alcian Blue Staining for Chondrocranium Development in Zebrafish
Craniofacial abnormalities are one of the most frequent birth malformations in humans, affecting around one in every thousand live births. The...
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Protargol Staining Technique for Identification of Soil Protists
Protargol staining technique is an excellent way to view the cellular organs of protists such as nuclei, cytostome, cilia, and flagella and to...
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Staining Technology and Bright-Field Microscope Use
We will introduce bright-field microscope use and practice Gram staining with foodborne pathogens and endospore staining with Bacillus cereus.
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Staining Starch with Iodine Solution
Starch is important material in plant tissues, especially for storage tissues. Starches from different plant resources or tissues vary in morphology,...
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SEM: Sample Preparation, Fixation, and Staining of Protists
The ultrastructure and morphology of the protist can be studied using high-resolution electron microscope, i.e., scanning electron microscope (SEM)....
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Detection of Endophytes by Reactive Oxygen Staining
Endophytes (bacteria or fungi) are colonizers of the internal plant tissues without causing any disease or hostile symptoms. Endophytes have...
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Prussian Blue Staining to Visualize Iron Oxide Nanoparticles
Iron deposits in cells and tissues can be detected by ex vivo histological examination through the Prussian blue (PB) staining. This practical,...
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Immunofluorescence staining of chloroplast proteins with frozen sections of plant tissues
Key messageImmunofluorescence staining with frozen sections of plant tissues and a nest tube is convenient and effective, and broadens the...
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Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries
Studies of the Drosophila ovary have provided significant insight into the molecular and cellular processes that control cell function, tissue...
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Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membrane
Mucins are often stained with the basic dye Alcian blue, but mucins with a low acidic glycan content cannot be stained with it. Succinylation-Alcian...
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Phalloidin Staining for F-Actin in Hepatic Stellate Cells
During the development of liver fibrosis, hepatic stellate cells undergo a transition from a quiescent phenotype into a proliferative, fibrogenic,...
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Advances in AMF Research: Isolation, Histochemical Staining, Enumeration, Morphological and Molecular Techniques
The mutualistic association of arbuscular mycorrhizal fungi (AMF) is an important symbiotic association between the roots of plant and fungi that...
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Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining
In the adult mouse testis, germ cells of various developmental cell states co-exist. FACS isolation of cells stained with the DNA dye Hoechst 33342...
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CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, an Alternative to LYMESPOT
Intracellular cytokine staining is a versatile technique used to analyze cytokine production in individual cells by flow cytometry. This methodology...
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Nontraditional Method for Telomere Staining by PNA Probes
The standard FISH uses DNA probes to hybridize to the designated complementary strands. This is DNA-DNA interaction, and it usually takes much longer...
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Perls/DAB Staining to Examine Iron Distribution in Arabidopsis Embryos
Iron is accumulated in Arabidopsis embryos during seed maturation. Where iron localizes in seed and embryo is important information for seed...