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Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system
BackgroundThe detailed transcriptomic profiles during human serotonin neuron (SN) differentiation remain elusive. The establishment of a reporter...
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Verification of CRISPR/Cas9 Activity In Vitro via SSA-Based Dual-Luciferase Reporter System
AbstractThe CRISPR/Cas9 technique has emerged as a powerful and promising tool for precise genomic integration, which applied to various cell types...
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Design and validation of a dual-fluorescence reporter system to monitor bacterial gene expression in the gut environment
AbstractFluorescence-based reporter systems are valuable tools for studying gene expression dynamics in living cells. However, available strategies...
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The GUS Reporter System in Flower Development Studies
The β-glucuronidase (GUS) reporter gene system is an important technique with versatile uses in the study of flower development in a broad range of...
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Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system
Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue,...
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A visualization reporter system for characterizing antibiotic biosynthetic gene clusters expression with high-sensitivity
The crisis of antibiotic resistance has become an impending global problem. Genome sequencing reveals that streptomycetes have the potential to...
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Development of dual reporter vector system for estimating translational activity of regulatory elements
BackgroundFor the needs of modern biotechnology, a quantitative approach to the control of regulatory elements at all stages of gene expression has...
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Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression
Most experimental methods for structural biology proceed in vitro and therefore the contribution of the intracellular environment on protein...
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A Pseudomonas aeruginosa-Suitable Fluorescent Reporter System for Analyzing Small RNA-Mediated Regulation of Target mRNAs
Bacterial small RNAs have emerged as modulators that play key roles in regulatory networks related to viability, environmental adaptation, and...
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Monitoring Effector Translocation with the TEM-1 Beta-Lactamase Reporter System: From Endpoint to Time Course Analysis
Among the bacterial secretion systems, the Type III, IV, and VI secretion systems enable bacteria to secrete proteins directly into a target cell....
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Residual stresses in adhesively bonded wood determined by a bilayer flexion reporter system
The application of adhesives in modern timber engineering often introduces moisture into the wood, leading to permanent residual stresses after...
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Quantification of Xylem-Specific Thermospermine-Dependent Translation of SACL Transcripts with Dual Luciferase Reporter System
Thermospermine (Tspm) is a polyamine found to play a crucial role in xylem development in Arabidopsis thaliana. Tspm promotes the translation of the...
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A Luciferase-EGFP Reporter System for the Evaluation of DNA Methylation in Mammalian Cells
AbstractDNA methylation is an essential epigenetic modification involved in numerous biological processes. Here, we present a cell-based system...
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A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene
BackgroundBioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life,...
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An effector–reporter system to study cellular signal transduction in strawberry fruit (Fragaria ananassa)
An effector–reporter system is a powerful tool used to study cellular signal transduction, but this technique has been traditionally used in...
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A sensitive mNeonGreen reporter system to measure transcriptional dynamics in Drosophila development
The gene regulatory network governing anterior–posterior axis formation in Drosophila is a well-established paradigm to study transcription in...
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Exploiting the Gal4/UAS System as Plant Orthogonal Molecular Toolbox to Control Reporter Expression in Arabidopsis Protoplasts
The ability of protein domains to fold independently from the rest of the polypeptide is the principle governing the generation of fusion proteins...
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Establishment of an Efficient Screening System in Gene Transformation of High Oleic Acid Peanut Using AhFAD2B as a Reporter Gene
AbstractDuring gene transformation, selection makers are important for efficient screening of the transgenic lines. The commonly used selection...
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A versatile reporter system for multiplexed screening of effective epigenome editors
The formation and function of highly specialized cells and tissues in a multicellular organism from a single genome are enabled through differential...
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Map** Regulatory Elements within 5' and 3' UTRs of SIGLEC15 with a Use of Reporter System
AbstractSiglec-15 is an immune suppressor with broad upregulation on various cancer types and has emerged as a potential target for cancer...
