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An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins
X-ray radiolytic labeling uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation. High flux density...
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Handling and Processing of Cyanobacterial Toxin Containing Pond and Lake Water Samples
Blue-green algae, or cyanobacteria, are a kind of prokaryotic cell that can photosynthesize because it contains the chlorophyll molecule. They were...
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Improvement of beauvericin production by Fusarium oxysporum AB2 under solid-state fermentation using an optimised liquid medium and co-cultures
The production of beauvericin (BEA) by Fusarium oxysporum AB2 in liquid medium (SmF) was compared to that on solid medium (SSF) on inert support...
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Technological Advancements for the Analysis of Phytochemical Diversity in Plants
Phytochemicals are bioactive non-nutrient plant compounds that are important for the plants and also provide a range of pharmacological activities....
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Identification of Stress-Responsive Metabolites in Plants Using an Untargeted Metabolomics Approach
Stress can affect different groups of plant metabolites and multiple signaling pathways. Untargeted metabolomics enables the collection of...
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Sugar-painting inspired branched ureido polymers as high-performance formaldehyde-free wood adhesive
Develo** multifunctional adhesives with strong adhesion, excellent water-resistance, sustainability, low cost, and simple production approach is...
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Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly (ADP-Ribose)
We describe a method for analyzing multiple products of PARylation by PARP1 and/or PARP2 using high-pressure liquid chromatography. The method...
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UHPLC-HRMS-Based Analysis of S-Hydroxymethyl-Glutathione, GSH, and GSSG in Human Cells
Glutathione (GSH) is one of the main antioxidant molecules present in cells. It harbors a thiol group responsible for sustaining cellular redox...
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Blood-brain barrier disruption defines the extracellular metabolome of live human high-grade gliomas
The extracellular microenvironment modulates glioma behaviour. It remains unknown if blood-brain barrier disruption merely reflects or functionally...
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A novel laccase from Trametes polyzona with high performance in the decolorization of textile dyes
Laccases are multicopper oxidases able to oxidize several phenolic compounds and find application in numerous industrial applications. Among laccase...
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Practical Considerations in Method Development for Gas Chromatography-Based Metabolomic Profiling
This chapter discusses the fundamentals of gas chromatography (GC) to improve method development for metabolic profiling of complex biological...
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Ionic liquid as an effective green media for the synthesis of (5Z, 8Z)-7H-pyrido[2,3-d]azepine derivatives and recycable Fe3O4/TiO2/multi-wall cabon nanotubes magnetic nanocomposites as high performance organometallic nanocatalyst
AbstractIn this research we investigated the preparation of new (5 Z , 8 Z )-7 H -pyrido[2,3- d ]azepine derivatives in high yields via multicomponent...
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Quantification of DNA Modifications Using Two-Dimensional Ultraperformance Liquid Chromatography Tandem Mass Spectrometry (2D-UPLC-MS/MS)
Our hereby presented methodology is suitable for reliable assessment of the most common DNA modifications which arise as a product of fundamental...
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Hydroxymethylation of technical lignins obtained from different pretreatments for preparation of high-performance rigid polyurethane foam
Technical lignins are promising renewable resources for the production of a variety of value-added products such as polyurethane. In this study,...
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Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
Extraction and quantification of endocannabinoids from biological tissues is essential to unravel their changes under physiological and...
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Biosensor-based high-throughput screening enabled efficient adipic acid production
AbstractAdipic acid is an industrially important chemical, but the current approach to synthesize it can be of serious pollution to the environment....
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Tensor methods in data analysis of chromatography/mass spectroscopy-based plant metabolomics
Plant metabolomics is an important research area in plant science. Chemometrics is a useful tool for plant metabolomic data analysis and processing....
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Carbon dioxide expanded liquid: an effective solvent for the extraction of quercetin from South African medicinal plants
BackgroundQuercetin is one of the most important bioflavonoids having positive effects on the biological processes and human health. Typically, it is...
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Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin
In monoclonal antibody (mAb) production, aggregates represent a major class of product-related impurities that needs to be removed by the downstream...
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Making Cocoa Origin Traceable
Nowadays, consumers have become aware of different cocoa genotypes and their origins, which caused an expanding market of premium chocolates with...