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A donor-DNA-free CRISPR/Cas-based approach to gene knock-up in rice
Structural variations (SVs), such as inversion and duplication, contribute to important agronomic traits in crops
1 . Pan-genome studies revealed that...
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Arms and ammunitions: effectors at the interface of rice and it’s pathogens and pests
The plant immune system has evolved to resist attack by pathogens and pests. However, successful phytopathogens deliver effector proteins into plant...
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Protoplast Cultures
Protoplasts are used to investigate a broad range of physiological problems reaching from the significance of the cell wall for nutrient uptake to...
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Development of an efficient protoplast isolation and transfection system for castor bean (Ricinus communis L.)
Castor bean is an oil crop plant (Euphorbiaceae) found across the tropical, subtropical, and temperate regions. Despite its important oil properties...
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Agrobacterium-mediated rapid and efficient development of transgenics using shoot apex explants in two elite Indica rice cultivars
An efficient multiple shoot induction, plant regeneration, and subsequent genetic transformation system for two locally adapted recalcitrant indica...
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Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts
CRISPR-Cas9 has revolutionized the field of genome engineering. Base editing, a new genome editing strategy, was recently developed to engineer...
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Fine-Tuning of the Grain Size by Alternative Splicing of GS3 in Rice
Grain size is subtly regulated by multiple signaling pathways in rice. Alternative splicing is a general mechanism that regulates gene expression at...
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OsMYB103 is essential for tapetum degradation in rice
Key message
Abstract ...OsMYB103 positively regulates tapetum degradation, and functions downstream of TDR and upstream of EAT1 and PTC1 .
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Genetic Engineering for Biotic Stress Management in Rice
Rice yield is affected by various biotic stresses including fungi, bacteria, viruses, parasites, nematodes, weeds and insects, posing a major threat...
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OsFTL4, an FT-like Gene, Regulates Flowering Time and Drought Tolerance in Rice (Oryza sativa L.)
The initiation of flowering in cereals is a critical process influenced by environmental and endogenous signals. Flowering Locus T-like ( FT-like )...
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The Amino Acid Transporter OsAAP4 Contributes to Rice Tillering and Grain Yield by Regulating Neutral Amino Acid Allocation through Two Splicing Variants
BackgroundAmino acids, which are transported by amino acid transporters, are the major forms of organic nitrogen utilized by higher plants. Among the...
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Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani
Rhizoctonia solani is an important phytopathogenic fungus with a wide host range and worldwide distribution. The anastomosis group AG1 IA of R. solani ...
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Reconstitution of the Core ABA Signaling in Protoplasts : Transcriptional Activators
Transient expression of genes in protoplasts has been used widely for purposes ranging from subcellular localization to promoter activity analyses....
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Subcellular Localization of the D27 Protein in Sugarcane (Saccharum spp. Hybrids) Using an Optimized Protoplast Isolation, Purification, and Transient Gene Expression Protocol
The transient gene expression of protoplasts in plant is a considerable tool for gene functional research that has been widely used in gene analysis...
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Chlorosis seedling lethality 1 encoding a MAP3K protein is essential for chloroplast development in rice
BackgroundMitogen-activated protein kinase (MAPK) cascades are conserved signaling modules in eukaryotic organisms and play essential roles in...
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Rice histone deacetylase HDA704 positively regulates drought and salt tolerance by controlling stomatal aperture and density
Main conclusionHDA704 enhances drought and salt tolerance via stomata-regulated mechanism. HDA704 negatively regulates stomatal aperture and density,... 
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Nutritional analysis and molecular characterization of hybrid mushrooms developed through intergeneric protoplast fusion between Pleurotus sajor-caju and Calocybe indica with the purpose to achieve improved strains
Two edible mushrooms Calocybe indica and Pleurotus sajor-caju were chosen as parent strains in this study to approach the concept of hybridization...
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OsFLA1 encodes a fasciclin-like arabinogalactan protein and affects pollen exine development in rice
Key messageOsFLA1 positively regulates pollen exine development, and locates in the cellular membrane.
AbstractArabinogalactan proteins are a type...
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Transcriptomics, metabolomics, antioxidant enzymes activities and respiration rate analysis reveal the molecular responses of rice to Cd stress and/or elevated CO2 concentration
PurposeTo explore the regulatory mechanism of rice to Cd stress and/or elevated CO 2 concentration.
MethodsThe rice seedlings ( Oryza sativa L.) were...
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An Adenylate Kinase OsAK3 Involves Brassinosteroid Signaling and Grain Length in Rice (Oryza sativa L.)
BackgroundGrain size is one of the major determinants of cereal crop yield. As a class of plant polyhydroxysteroids, brassinosteroids (BRs) play...
