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The effect of temperature on the activity and stability of the thermostable enzyme caffeine dehydrogenase from Pichia manshurica CD1
A novel thermostable caffeine dehydrogenase enzyme was isolated from Pichia manshurica strain CD1. At increased temperatures, the enzyme caffeine...
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Structure-based mining of a chitosanase with distinctive degradation mode and product specificity
AbstractChitosan derivates with varying degrees of polymerization (DP) have attracted great concern due to their excellent biological activities....
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Improving the specificity of nucleic acid detection with endonuclease-actuated degradation
Nucleic acid detection is essential for numerous biomedical applications, but often requires complex protocols and/or suffers false-positive...
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Insights into glycosidic bond specificity of an engineered selective α-L-rhamnosidase N12-Rha via activity assays and molecular modelling
αL-rhamnosidase (EC 3.2.1.40) has been widely used in food processing and pharmaceutical preparation. The recombinant α-L-rhamnosidase N12-Rha from Asp...
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Mating pair stabilization mediates bacterial conjugation species specificity
Bacterial conjugation mediates contact-dependent transfer of DNA from donor to recipient bacteria, thus facilitating the spread of virulence and...
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Map** enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons
Simultaneous spatial map** of the activity of multiple enzymes in a living system can elucidate their functions in health and disease. However,...
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Dencichine attenuates the virulence of Fusobacterium nucleatum by targeting hydrogen sulfide-producing enzyme
Oral opportunistic pathogen Fusobacterium nucleatum can participate in various disease processes through the metabolite hydrogen sulfide, such as...
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Construction of immobilized enzyme cascades for the biosynthesis of nucleotide sugars UDP-N-acetylglucosamine and UDP-glucuronic acid
UDP-sugars, as active forms of monosaccharides, play integral roles in glycosylation and biosynthesis of polysaccharides. Although enzymatic...
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Enhancing Enzyme Activity and Thermostability of Bacillus amyloliquefaciens Chitosanase BaCsn46A Through Saturation Mutagenesis at Ser196
Chitosanase plays an important role in chitooligosaccharides (COS) production. We found that the chitosanase (BaCsn46A) of Bacillus amyloliquefacien ...
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Autoantibody Recognition of Natural and Homocysteinylated Alpha-1 Antitrypsin: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Quantification in Sera
In this chapter, indirect enzyme-linked immunosorbent assays (ELISAs) to quantitatively measure autoantibodies directed to human natural and...
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Molecular and structural basis of an ATPase-nuclease dual-enzyme anti-phage defense complex
Coupling distinct enzymatic effectors emerges as an efficient strategy for defense against phage infection in bacterial immune responses, such as the...
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Classification and Enzyme Properties of Starch Debranching Enzymes
The α-1,6 glycosidic linkages in polysaccharides, including amylose, straight-chain starch, pullulan, and glycogen, are hydrolyzed by starch...
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New insights to diversity and enzyme–substrate interactions of fungal glucuronoyl esterases
AbstractGlucuronoyl esterases (GEs) (EC 3.1.1.117) catalyze the cleavage of ester-linked lignin-carbohydrate complexes that has high impact on the...
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Genome-wide identification of RUB activating enzyme and conjugating enzyme gene families and their expression analysis under abiotic stresses in Capsicum annuum
NEDD8/RUB, as a ubiquitin-like protein, participates in the post-translational modification of protein and requires unique E1, E2, and E3 enzymes to...
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Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein
We conducted a development and standardization of an IgG ELISA assay for serological detection of human orthohantavirus infections using the...
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Specificity Analysis of Protein Methyltransferases and Discovery of Novel Substrates Using SPOT Peptide Arrays
Posttranslational methylation of amino acid side chains in proteins mainly occurs on lysine, arginine, glutamine, and histidine residues. It is...
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Enzyme dynamics—a brief review
Debate has been simmering for some years regarding the importance of internal thermal motions of enzymes to catalysis. Recent developments in protein...
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CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue
BackgroundPost mortem human brain tissue is an essential resource to study cell types, connectivity as well as subcellular structures down to the...
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Enhancement of α-galactosidase production using novel Actinoplanes utahensis B1 strain: sequential optimization and purification of enzyme
α-Galactosidase is an important exoglycosidase belonging to the hydrolase class of enzymes, which has therapeutic and industrial potential. It plays...
