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Transient virus-induced gene silencing of MaBAM9b efficiently suppressed starch degradation during postharvest banana fruit ripening
The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be...
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Transcriptomic analysis of wheat reveals possible resistance mechanism mediated by Yr10 to stripe rust
Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a catastrophic disease that threatens global wheat yield. Yr10 is a...
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Genome wide identification of GDSL gene family explores a novel GhirGDSL26 gene enhancing drought stress tolerance in cotton
BackgroundCurrent climate change scenarios are posing greater threats to the growth and development of plants. Thus, significant efforts are required...
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GbC4H regulates the metabolic flow of flavonoids and inhibits the occurrence of Fusarium wilt in sea island cotton
Fusarium wilt is a soil-borne fungal disease, and various problems associated with this disease in cotton production in **njiang need to be urgently...
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Genome-wide identification of GhRLCK-VII subfamily genes in Gossypium hirsutum and investigation of their functions in resistance to Verticillium wilt
BackgroundThe receptor-like cytoplasmic kinases subfamily VII (RLCK-VII) is critical in regulating plant growth, development, and pattern-triggered...
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Ocimum sanctum, OscWRKY1, regulates phenylpropanoid pathway genes and promotes resistance to pathogen infection in Arabidopsis
Key messageOscWRKY1 from Ocimum sanctum positively regulates phenylpropanoid pathway genes and rosmarinic acid content. OscWRKY1 overexpression...
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Genome-wide association study reveals that GhTRL1 and GhPIN8 affect cotton root development
Key messageTwo regions located at chromosome A05 and D04 were found to be significantly associated with 0–0.5 mm and 0.5–2 mm diameter roots,...
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A mutation in CsGME encoding GDP-mannose 3,5-epimerase results in little and wrinkled leaf in cucumber
Key messageMap-based cloning revealed that a mutation in a highly conserved amino acid of the CsGME gene encoding GDP-mannose 3,5-epimerase, causes...
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Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus
BackgroundMaize dwarf mosaic virus (MDMV), a member of the genus Potyvirus , infects maize and is non-persistently transmitted by aphids. Several...
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GhGASA14 regulates the flowering time of upland cotton in response to GA3
Key messageThe silencing of
GhGASA14 and the identification of superior allelic variation in its coding region indicate thatGhGASA14 may positively... -
Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)
Water dropwort ( Oenanthe javanica (Blume) DC), an aquatic perennial plant from the Apiaceae family, rich in dietary fibert, vitamins, and minerals....
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Roles of small RNAs in crop disease resistance
Small RNAs (sRNAs) are a class of short, non-coding regulatory RNAs that have emerged as critical components of defense regulatory networks across...
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GhIMP10D, an inositol monophosphates family gene, enhances ascorbic acid and antioxidant enzyme activities to confer alkaline tolerance in Gossypium hirsutum L.
BackgroundInositol monophosphates (IMP) are key enzymes in the ascorbic acid (AsA) synthesis pathways, which play vital roles in regulating plant...
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Development of a Csy4-Processing TRV-Based CRISPR/Cas9 Genome Editing System in Nicotiana benthamiana
The CRISPR/Cas9 system is a site-specific genome editing tool that has been widely used in various plant species. The plant virus-based gRNA (guide...
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Structure and character analysis of cotton response regulator genes family reveals that GhRR7 responses to draught stress
BackgroundCytokinin signal transduction is mediated by a two-component system (TCS). Two-component systems are utilized in plant responses to...
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Production of Double-Stranded RNA in Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing
RNA silencing (also known as gene silencing) is an evolutionary conserved mechanism that is involved in regulating gene expression, suppressing... -
Simultaneous silencing of two different Arabidopsis genes with a novel virus-induced gene silencing vector
BackgroundVirus-induced gene silencing (VIGS) is a useful tool for functional characterizations of plant genes. However, the penetrance of VIGS...
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Nanoparticle-mediated dsRNA delivery for precision insect pest control: a comprehensive review
Nanoparticle-based delivery systems have emerged as powerful tools in the field of pest management, offering precise and effective means of...
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Knockdown of 60S ribosomal protein L14-2 reveals their potential regulatory roles to enhance drought and salt tolerance in cotton
BackgroundCotton is a valuable economic crop and the main significant source of natural fiber for textile industries globally. The effects of drought...
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Overexpression and VIGS system for functional gene validation in oriental melon (Cucumis melo var. makuwa Makino)
As one of the effective and powerful methods for gene function analysis in plants, transient overexpression and virus-induced gene silencing (VIGS)...