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ISSAAC-seq enables sensitive and flexible multimodal profiling of chromatin accessibility and gene expression in single cells
Joint profiling of chromatin accessibility and gene expression from the same single cell provides critical information about cell types in a tissue...
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Single-cell lineage capture across genomic modalities with CellTag-multi reveals fate-specific gene regulatory changes
Complex gene regulatory mechanisms underlie differentiation and reprogramming. Contemporary single-cell lineage-tracing (scLT) methods use expressed,...
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Microfluidics-free single-cell genomics with templated emulsification
Current single-cell RNA-sequencing approaches have limitations that stem from the microfluidic devices or fluid handling steps required for sample...
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Single-nucleotide variant calling in single-cell sequencing data with Monopogen
Single-cell omics technologies enable molecular characterization of diverse cell types and states, but how the resulting transcriptional and...
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Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos
The development of single-cell multiomics has provided the ability to systematically investigate cellular diversity and heterogeneity in different...
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Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach
Global gene expression profiling has provided valuable insights into the specific contributions of different cell types to various physiological...
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Tumor cell type and gene marker identification by single layer perceptron neural network on single-cell RNA sequence data
Tumors have drawn increasing attention recently because of their heterogeneous interior structures. Particularly, single-cell RNA (scRNA) mechanics...
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Single-cell profiling of vascular endothelial cells reveals progressive organ-specific vulnerabilities during obesity
Obesity promotes diverse pathologies, including atherosclerosis and dementia, which frequently involve vascular defects and endothelial cell (EC)...
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Cellular basis of enhanced humoral immunity to SARS-CoV-2 upon homologous or heterologous booster vaccination analyzed by single-cell immune profiling
SARS-CoV-2 vaccine booster dose can induce a robust humoral immune response, however, its cellular mechanisms remain elusive. Here, we investigated...
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Recovery of missing single-cell RNA-sequencing data with optimized transcriptomic references
Single-cell RNA-sequencing (scRNA-seq) is an indispensable tool for characterizing cellular diversity and generating hypotheses throughout biology....
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Time-resolved assessment of single-cell protein secretion by sequencing
Secreted proteins play critical roles in cellular communication. Methods enabling concurrent measurement of cellular protein secretion, phenotypes...
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Single-cell transcriptomics: background, technologies, applications, and challenges
Single-cell sequencing was developed as a high-throughput tool to elucidate unusual and transient cell states that are barely visible in the bulk....
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Exosomal MicroRNA Profiling
Exosomes are extracellular vesicles, which have the ability to convey various types of cargo between cells. Lately, a great amount of interest has...
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cfDNA methylome profiling for detection and subty** of small cell lung cancers
Small cell lung cancer (SCLC) is characterized by morphologic, epigenetic and transcriptomic heterogeneity. Subtypes based upon predominant...
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Integrating Single-Cell Methylome and Transcriptome Data with MAPLE
As a mechanism of epigenetic gene regulation, DNA methylation has crucial roles in developmental and differentiation programs. Thanks to the recently...
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scPCOR-seq enables co-profiling of chromatin occupancy and RNAs in single cells
Cell-to-cell variation in gene expression is a widespread phenomenon, which may play important roles in cellular differentiation, function, and...
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A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility
Profiling chromatin accessibility at the single-cell level provides critical information about cell type composition and cell-to-cell variation...
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TISSUE: uncertainty-calibrated prediction of single-cell spatial transcriptomics improves downstream analyses
Whole-transcriptome spatial profiling of genes at single-cell resolution remains a challenge. To address this limitation, spatial gene expression...
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The single-cell chromatin landscape in gonadal cell lineage specification
Gonad development includes sex determination and divergent maturation of the testes and ovaries. Recent advances in measuring gene expression in...
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Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin
Recent efforts have succeeded in surveying open chromatin at the single-cell level, but high-throughput, single-cell assessment of heterochromatin...
