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Protoplast Cultures
Protoplasts are used to investigate a broad range of physiological problems reaching from the significance of the cell wall for nutrient uptake to...
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Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling
BackgroundSalsola laricifolia is a typical C 3 –C 4 typical desert plant, belonging to the family Amaranthaceae . An efficient single-cell system is...
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Nutritional analysis and molecular characterization of hybrid mushrooms developed through intergeneric protoplast fusion between Pleurotus sajor-caju and Calocybe indica with the purpose to achieve improved strains
Two edible mushrooms Calocybe indica and Pleurotus sajor-caju were chosen as parent strains in this study to approach the concept of hybridization...
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Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana
BackgroundTo date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana. Here, the establishment of a...
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Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii
Early studies revealed a highly predictable pattern of gravity-directed growth and development in Ceratopteris richardii spores. This makes the spore...
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Quarantine of Germplasm: Safeguarding Genetic Resources Through In Vitro Tissue Culture
The international transfer of beneficial germplasm has played a crucial role in the advancement of agricultural diversification, with around 30% of...
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Tissue culture-based genetic improvement of fava bean (Vicia faba L.): analysis on previous achievements and future perspectives
Fava bean is an extremely important legume and serves immense potential to function as an ingredient as pulse proteins in human diet. Bearing the...
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Subcellular Localization of the D27 Protein in Sugarcane (Saccharum spp. Hybrids) Using an Optimized Protoplast Isolation, Purification, and Transient Gene Expression Protocol
The transient gene expression of protoplasts in plant is a considerable tool for gene functional research that has been widely used in gene analysis...
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Characterization of allotetraploids derived from protoplast fusion between navel orange and kumquat
In this study, we aimed to produce a citrus fruit with no seed and with edible peel. Therefore, the seedless Yoshida navel oranges ( Citrus sinensis...
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Cell and Protoplast Culture for Production of Plant Metabolites
Plant secondary metabolites are biosynthetically derived from primary metabolites. Recent surveys have established that nearly 35% of molecules in...
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An efficient embryogenic cell suspension culture system through secondary somatic embryogenesis and regeneration of true-to-type plants in banana cv. Sabri (silk subgroup AAB)
A reliable and reproducible embryogenic cell suspension culture system established successfully for regeneration of plants using pro-embryogenic cell...
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Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera
BackgroundCamellia oleifera ( C. oleifera ) is a woody edible oil crop of great economic importance. Because of the lack of modern biotechnology...
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Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing
The CRISPR/Cas9-based gene editing system for the direct delivery of pre-assembled Cas9 ribonucleoproteins (RNPs), consisting of a Cas9 nuclease and...
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Editing of the ethylene biosynthesis gene in carnation using CRISPR-Cas9 ribonucleoprotein complex
The study aimed to edit ethylene (ET) biosynthesis genes [ 1-aminocyclopropane-1-carboxylic acid (ACC) synthetase 1 ( ACS1 ) and ACC oxidase 1 ( ACO1 )]...
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In Vitro Culture for Micropropagation, Somatic Embryogenesis, Somatic Mutation, and Somatic Hybridization in Brassica juncea
Development of Brassica juncea in vitro regeneration protocol can be a useful tool for breeders to select genotypes having interesting traits which...
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Optimization of protoplast isolation from the gametophytes of brown alga Undaria pinnatifida using response surface methodology
The microscopic gametophytic phase of the commercial brown alga, Undaria pinnatifida , can be used for several applications, including the production...
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Catabolic reprogramming of Brassica rapa leaf mesophyll protoplasts during the isolation procedure
The use of leaf mesophyll protoplasts for transformation and genome editing in plants is expected due to the distinctive features of protoplasts,...
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Colour change in potato (Solanum tuberosum L.) tubers by disruption of the anthocyanin pathway via ribonucleoprotein complex delivery of the CRISPR/Cas9 system
Potato is an important part of the traditional Norwegian diet, and the crop faces several challenges with respect to pests and diseases, as well as...
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Maize tissue culture, transformation, and genome editing
The importance of maize ( Zea mays L.) to global agriculture, world economy, and food security is widely known and increasing. Current maize breeding...
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Improvement of vincamine production of endophytic fungus through inactivated protoplast fusion
Improvement of the production of vincamine in endophytic fungus VINI-7 was performed by using the inactivated protoplast fusion method. The...
