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On-column refolding and off-column assembly of parvovirus B19 virus-like particles from bacteria-expressed protein
AbstractVirus-like particles (VLPs) are nanometric structures composed of structural components of virions, kee** most of the cellular recognition...
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Recent Progress in Structural Studies on the GT-C Superfamily of Protein Glycosyltransferases
Protein glycosylation is an essential covalent modification involved in protein secretion, stability, binding, folding, and activity. One or more...
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Protein profiling and immunoinformatic analysis of the secretome of a metal-resistant environmental isolate Pseudomonas aeruginosa S-8
The bacterial secretome represents a comprehensive catalog of proteins released extracellularly that have multiple important roles in virulence and...
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Cryo-EM structure of the bacterial divisome core complex and antibiotic target FtsWIQBL
In most bacteria, cell division relies on the synthesis of new cell wall material by the multiprotein divisome complex. Thus, at the core of the...
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Identification of residues for chaperone-like activity of OppA protein in Yersinia pseudotuberculosis
Periplasmic oligopeptide binding protein (OppA) is part of a multimeric cytoplasmic membrane protein complex, whose function is known as peptide...
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Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation
AbstractSecretion of bacterial proteins into the culture medium simplifies downstream processing by avoiding cell disruption for target protein...
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Cell Fractionation
Protein function is generally dependent on its subcellular localization. In gram-negative bacteria such as Escherichia coli, a protein can be...
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Components Subcellular Localization: Cell Surface Exposure
Surface-exposed proteins of Gram-negative bacteria are represented by integral outer membrane β-barrel proteins and lipoproteins. There are no...
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Trichlorobacter ammonificans, a dedicated acetate-dependent ammonifier with a novel module for dissimilatory nitrate reduction to ammonia
Dissimilatory nitrate reduction to ammonia (DNRA) is a common biochemical process in the nitrogen cycle in natural and man-made habitats, but its...
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X-ray crystallography reveals molecular recognition mechanism for sugar binding in a melibiose transporter MelB
Major facilitator superfamily_2 transporters are widely found from bacteria to mammals. The melibiose transporter MelB, which catalyzes melibiose...
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Bacterial Sialic Acid Catabolism at the Host–Microbe Interface
Sialic acids consist of nine-carbon keto sugars that are commonly found at the terminal end of mucins. This positional feature of sialic acids...
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Kinetic consequences of the endogenous ligand to molybdenum in the DMSO reductase family: a case study with periplasmic nitrate reductase
AbstractThe molybdopterin enzyme family catalyzes a variety of substrates and plays a critical role in the cycling of carbon, nitrogen, arsenic, and...
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Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens
AbstractBacterial outer membrane vesicles (OMVs) are nanosized spheroidal particles shed by gram-negative bacteria that contain biomolecules derived...
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Analysis of Activity of Human Steroidogenic Acute Regulatory Protein (STARD1) Expressed in Escherichia coli Cells
AbstractOne of the main obstacles to the successful use of Escherichia coli cells for steroid transformation in biotechnological processes is...
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Periplasmic production of pernisine in Escherichia coli and determinants for its high thermostability
AbstractPernisine is a subtilisin-like serine proteinase secreted by the hyperthermophilic archaeon Aeropyrum pernix . The significant properties of...
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Generation and functional analysis of single chain variable fragments (scFvs) targeting the nucleocapsid protein of Porcine epidemic diarrhea virus
AbstractPorcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea, which can cause death in suckling piglets....
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Purification of the Na+-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy
The flagellar motor of marine Vibrio is driven by the sodium-motive force across the inner membrane. The stator complex, consisting of two membrane...
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Cryo-EM structure of transmembrane AAA+ protease FtsH in the ADP state
AAA+ proteases regulate numerous physiological and cellular processes through tightly regulated proteolytic cleavage of protein substrates driven by...
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Bacterial One- and Two-Hybrid Assays to Monitor Transmembrane Helix Interactions
In transenvelope multiprotein machines such as bacterial secretion systems, protein–protein interactions not only occur between soluble domains but...
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Exploring Uniform, Dual, and Dynamic Topologies of Membrane Proteins by Substituted Cysteine Accessibility Method (SCAM™)
A described simple and advanced protocol for Substituted Cysteine Accessibility Method as applied to transmembrane (TM) orientation (SCAM™) permits a...