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Structural Significance of Conformational Preferences and Ribose-Ring-Puckering of Hyper Modified Nucleotide 5’-Monophosphate 2-Methylthio Cyclic N6-Threonylcarbamoyladenosine (p-ms2ct6A) Present at 37th Position in Anticodon Loop of tRNALys
Structural significance of conformational preferences and ribose ring puckering of newly discovered hyper modified nucleotide, 5’-monophosphate...
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Combining Biophysical Methods for Structure–Function Analyses of RNA in Solution
RNA-level regulation by riboswitches relies on the specific binding of small metabolites to the aptamer domain to trigger substantial conformational...
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Protein structure analysis of the interactions between SARS-CoV-2 spike protein and the human ACE2 receptor: from conformational changes to novel neutralizing antibodies
The recent severe acute respiratory syndrome, known as Coronavirus Disease 2019 (COVID-19) has spread so much rapidly and severely to induce World...
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Characterizing Biophysical Parameters of Single TCR-pMHC Interactions Using Optical Tweezers
αβ T cells are mechanosensors that leverage bioforces during immune surveillance for highly sensitive and specific antigen discrimination....
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Characterization of Complexes and Supramolecular Structures by Electron Microscopy
Recent advancements in cryo-electron microscopy (cryo-TEM) have enabled the determination of structures of macromolecular complexes at near-atomic...
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Easy Not Easy: Comparative Modeling with High-Sequence Identity Templates
Homology modeling is the most common technique to build structural models of a target protein based on the structure of proteins with high-sequence...
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Measurement of Integrin Activation and Conformational Changes on the Cell Surface by Soluble Ligand and Antibody Binding Assays
Soluble ligand and conformation-dependent antibody binding assay of integrins on the cell surface is an effective approach to evaluate the activation...
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Molecular Dynamics Simulation to Study Protein Conformation and Ligand Interaction
The field of molecular dynamics (MD) simulations has become indispensable today to studying the conformational flexibility and dynamics of proteins...
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New Fluorescent Analogs of Nucleotides Based on 3-Hydroxychromone for Recording Conformational Changes of DNA
AbstractIt has recently been found that derivatives of nucleotides containing а 3-hydroxychromone fluorescent dye can be used as sensitive markers of...
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Inhibition mechanisms of CRISPR-Cas9 by AcrIIA25.1 and AcrIIA32
In the ongoing arms race between bacteria and bacteriophages, bacteriophages have evolved anti-CRISPR proteins to counteract bacterial CRISPR-Cas...
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Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching
In this study, fluorescence recovery after photobleaching (FRAP) experiments were performed on RBC labeled by lipophilic fluorescent dye CM-DiI to...
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Probing Protein Topology and Conformation by Limited Proteolysis
Proteases are enzymes that catalyze the hydrolytic degradation of other proteins into peptides or amino acids through the digestion of the peptide...
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Histidine-Lacked Aβ(1–16) Peptides: pH-Dependent Conformational Changes in Metal Ion Binding
The region 1–16 of the amyloid-β peptides associated with Alzheimer’s disease can be considered as the metal-binding domain, and the residues His 6 ,...
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Energetics of ångström-scale conformational changes in an RCK domain of the MthK K+ channel
Allosteric proteins transition among different conformational states in a ligand-dependent manner. Upon resolution of a protein’s individual states,...
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A pleurocidin analogue with greater conformational flexibility, enhanced antimicrobial potency and in vivo therapeutic efficacy
Antimicrobial peptides (AMPs) are a potential alternative to classical antibiotics that are yet to achieve a therapeutic breakthrough for treatment...
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Structures and Electron Transport Paths in the Four Families of Flavin-Based Electron Bifurcation Enzymes
Oxidoreductases facilitating electron transfer between molecules are pivotal in metabolic pathways. Flavin-based electron bifurcation (FBEB), a...
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Bacterial Secretion Systems Methods and Protocols
This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and...
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Comparative Analysis of Nucleotide Fluorescent Analogs for Registration of DNA Conformational Changes Induced by Interaction with Formamidopyrimidine-DNA Glycosylase Fpg
AbstractDNA-substrates containing fluorescent DNA base analogs are widely used to study protein–nucleic acid interactions. In the case of...
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Directed proton transfer from Fo to F1 extends the multifaceted proton functions in ATP synthase
The role of protons in ATP synthase is typically considered to be energy storage in the form of an electrochemical potential, as well as an operating...
