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The effect of probe geometry on rind puncture resistance testing of maize stalks
BackgroundStalk lodging (breaking of plant stems prior to harvest) is a major impediment to increasing agricultural yields of grain crops. Rind...
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Comparison of methylation capture sequencing and Infinium MethylationEPIC array in peripheral blood mononuclear cells
BackgroundEpigenome-wide association studies (EWAS) have been widely applied to identify methylation CpG sites associated with human disease. To...
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Detection of Listeria monocytogenes in foods with a textile organic electrochemical transistor biosensor
AbstractFoods contaminated by pathogens are responsible for foodborne diseases which have socioeconomic impacts. Many approaches have been...
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Imaging assay to probe the role of telomere length shortening on telomere-gene interactions in single cells
Telomeres are repetitive non-coding nucleotide sequences (TTAGGGn) cap** the ends of chromosomes. Progressive telomere shortening with increasing...
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Targeted design of green carbon dot-CA-125 aptamer conjugate for the fluorescence imaging of ovarian cancer cell
Aptamer-Carbon Dot (CD) bioconjugation is an attractive target-tracking strategy in detecting cell surface antigens. This study describes an...
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Streamlined Purification of RNA–Protein Complexes Using UV Cross-Linking and RNA Antisense Purification
RNA–protein interactions are important in development and disease, but identification of novel RNA–protein interactions remains challenging. Here, we...
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Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing
Single-cell CRISPR screens enable the exploration of mammalian gene function and genetic regulatory networks. However, use of this technology has...
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Development and validation of a RAD-Seq target-capture based genoty** assay for routine application in advanced black tiger shrimp (Penaeus monodon) breeding programs
BackgroundThe development of genome-wide genoty** resources has provided terrestrial livestock and crop industries with the unique ability to...
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Determining the Compaction State of Genes Using DNA FISH
DNA fluorescence in situ hybridization (FISH) enables the visualization of chromatin architecture and the interactions between genomic loci at a...
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Quantitative PCR method to detect an extremely endangered bitterling fish (Rhodeus atremius suigensis) using environmental DNA
Rhodeus atremius suigensis is an extremely endangered bitterling fish, designated as one of the Nationally Endangered Species of Wild Fauna and Flora...
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Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils
Eosinophils are a class of granulocytes with pleiotropic functions in homeostasis and various human diseases. Nevertheless, they are absent from...
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Single-cell analysis of EphA clustering phenotypes to probe cancer cell heterogeneity
The Eph family of receptor tyrosine kinases is crucial for assembly and maintenance of healthy tissues. Dysfunction in Eph signaling is causally...
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The Nodewalk assay to quantitate chromatin fiber interactomes in very small cell populations
The chromosome conformation capture method and its derivatives, such as circularized chromosome conformation capture, carbon copy chromosome...
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Predicting crop damage caused by wireworms and the effect of tillage on trap efficiency
A novel wireworm ‘probe’ trap is described, characterized, and used in field trials to (i) determine effects of different spring tillage treatments...
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Establishment of environmental DNA method for detecting invasive African-clawed frogs in Japan
African-clawed frogs ( Xenopus laevis ) are invasive to many aquatic ecosystems across the globe worldwide. For an accurate and efficient determination...
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Global map** of RNA G-quadruplexes (G4-RNAs) using G4RP-seq
Guanine-rich RNAs can fold into four-stranded structures, termed G-quadruplexes (G4-RNAs), and participate in a wide range of biological processes....
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Recurrent connectivity supports higher-level visual and semantic object representations in the brain
Visual object recognition has been traditionally conceptualised as a predominantly feedforward process through the ventral visual pathway. While...
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Dual spatially resolved transcriptomics for human host–pathogen colocalization studies in FFPE tissue sections
Technologies to study localized host–pathogen interactions are urgently needed. Here, we present a spatial transcriptomics approach to simultaneously...
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Characterisation and reproducibility of the HumanMethylationEPIC v2.0 BeadChip for DNA methylation profiling
BackgroundThe Illumina family of Infinium Methylation BeadChip microarrays has been widely used over the last 15 years for genome-wide DNA...
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Electrodeposited magnetic nanoporous membrane for high-yield and high-throughput immunocapture of extracellular vesicles and lipoproteins
Superparamagnetic nanobeads offer several advantages over microbeads for immunocapture of nanocarriers (extracellular vesicles, lipoproteins, and...
