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Abstracts from the 3rd International Genomic Medicine Conference (3rd IGMC 2015)
O1 Regulation of genes by telomere length over long distances
Jerry W. Shay
O2 The microtubule destabilizer KIF2A regulates the postnatal establishment...
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Treatment of Male Infertility
Major difficulties exist in the accurate and meaningful diagnosis of male reproductive dysfunction, and our understanding of the epidemiology and...
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Sex hormonal regulation and hormesis in aging and longevity: role of vitagenes
Aging process is accompanied by hormonal changes characterized by an imbalance between catabolic hormones, such as cortisol and thyroid hormones...
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Possible Effects of Metallosis on Spermatozoal Apoptotic Genes Expression in Individuals with Intramedullary Nailing Prosthesis
Seminal quality could be affected by metallosis caused by intramedullary nailing (IMN). Our objectives were to estimate metal ion levels in the...
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Methods of Sperm Vitality Assessment
Sperm vitality is a reflection of the proportion of live, membrane-intact spermatozoa determined by either dye exclusion or osmoregulatory capacity...
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Seminal plasma induces the expression of IL-1α in normal and neoplastic cervical cells via EP2/EGFR/PI3K/AKT pathway
BackgroundCervical cancer is a chronic inflammatory disease of multifactorial etiology usually presenting in sexually active women. Exposure of...
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Novel Markers of Male Infertility
Diagnostic tests should detect disease, have prognostic value, and aid in clinical decision making. Nowhere else in laboratory medicine does one have...
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A MALDI MS Investigation of the Lysophosphatidylcholine/Phosphatidylcholine Ratio in Human Spermatozoa and Erythrocytes as a Useful Fertility Marker
The human spermatozoa membrane is characterized by a unique fatty acyl composition with significant amounts of highly unsaturated fatty acids,...
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Recovery, Isolation, Identification, and Preparation of Spermatozoa from Human Testis
In some cases, human spermatozoa to be used for in vitro fertilization are processed from testicular or epididymal biopsies collected in the clinic...
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Understanding the Spermatozoon
The former perception of the spermatozoon as a delivery device of the male genome has been expanded to include a new understanding of the cell’s...
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Probabilistic alignment leads to improved accuracy and read coverage for bisulfite sequencing data
BackgroundDNA methylation has been linked to many important biological phenomena. Researchers have recently begun to sequence bisulfite treated DNA...
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TRIM32-dependent transcription in adult neural progenitor cells regulates neuronal differentiation
In the adult mammalian brain, neural stem cells in the subventricular zone continuously generate new neurons for the olfactory bulb. Cell fate...
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Spermatogenesis Methods and Protocols
Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process...
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Methods for Sperm Concentration Determination
Proper assessment of the number of spermatozoa is essential not only as an initial step in every clinical infertility investigation [Björndahl et al...
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Manual Methods for Sperm Motility Assessment
Progressive motility is a vital functional characteristic of ejaculated human spermatozoa that governs their ability to penetrate into, and migrate...
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Methods for the Detection of ROS in Human Sperm Samples
Male-factor significantly contributes to infertility in couples of reproductive age. Several studies have shown that oxidative stress (OS) is...
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Flow Cytometric Methods for Sperm Assessment
Flow cytometry allows the assessment of multiple sperm parameters following a diverse number of protocols. Here, we describe three methods for...
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Ultrastructural Analysis of Testicular Tissue and Sperm by Transmission and Scanning Electron Microscopy
Transmission electron microscopy (TEM) studies have provided the basis for an in-depth understanding of the cell biology and normal functioning of...
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Sex hormones and skeletal muscle weakness
Human ageing is accompanied with deterioration in endocrine functions the most notable and well characterized of which being the decrease in the...
