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Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
CRISPR-cas9-guided adenine base editors (ABEs) site-specifically convert the A-T base pair to G-C base pair in genomic DNA. The intracellular...
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Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae
Membrane protein (MP) functional and structural characterization requires large quantities of high-purity protein for downstream studies. Barriers to...
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Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy
Phage therapy is an alternative approach to combat bacterial infections. In this approach, bacteriophages are used as antimicrobial agents due to...
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Affinity Purification of Ubiquitinated Proteins Using p62-Agarose to Assess Ubiquitination of Clade A PP2Cs
Certain E3 ubiquitin ligases play a key role in the abscisic acid (ABA) pathway by targeting clade A type 2C protein phosphatases (PP2Cs) for...
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Low-affinity SPL binding sites contribute to subgenome expression divergence in allohexaploid wheat
Expression divergence caused by genetic variation and crosstalks among subgenomes of the allohexaploid bread wheat ( Triticum aestivum. L., BBAADD) is...
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Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass Spectrometry
The combination of advanced mass spectrometry and enrichment-based sample preparation methods has enhanced analytical capabilities in clinical...
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Recombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes
Many biosynthetic transformations have strict spatial and temporal requirements that necessitate the physical association of multiple enzymes for...
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CDR grafting and site-directed mutagenesis approach for the generation and affinity maturation of Anti-CD20 nanobody
BackgroundRecently, new and advanced techniques have been adopted to design and produce nanobodies, which are used in diagnostic and immunotherapy...
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Purification and biochemical characterization of phytase from Bacillus cereus isolated from gastrointestinal tract of African giant snail (Achatina fulica)
Phytases are specialized enzymes meant for phytic acid degradation. They possess ability to prevent phytic acid indigestion, including its attendant...
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E6 Tagged Protein Production, Extraction, and Purification from Escherichia coli Lysate
Cervical cancer has been extensively associated with human papillomavirus (HPV) due to the expression of oncoproteins such as E6. This protein can...
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In-house protocol: spin-based viral RNA purification
A worldwide shortage of molecular biology consumables is in surge. This includes filter tips, nucleic acid purification kits, polymerases,...
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Purification of Prospective Vaccine Antigens from Gram-Positive Pathogens by Immunoprecipitation
Immunoprecipitation is an affinity purification technique that exploits the highly specific interactions formed between antibodies and their cognate...
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Expression, purification, characterization, and cytotoxic evaluation of the ML1-STxB fusion protein
Targeted delivery of a toxin substance to cancer cells is one of the most recent cancer treatment options. Mistletoe Lectin-1 (ML1) in Viscum album ...
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DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids
The interaction of proteins with DNA plays a central role in gene regulation. We describe a DNA affinity purification method that allows for...
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A Simple Purification Method for Heat-Stable Recombinant Low Molecular Weight Proteins and Peptides Via GST-Fusion Products
Here, we describe a simple, rapid, cost-effective, and efficient novel one-step purification method for GST-tagged peptides and small proteins. This...
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Expression and purification of epinecidin-1 variant (Ac-Var-1) by acid cleavage
AbstractThe demand for massive quantities of therapeutic active antimicrobial peptides (AMPs) is high due to their potential as alternatives to...
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Combined proximity labeling and affinity purification−mass spectrometry workflow for map** and visualizing protein interaction networks
Affinity purification coupled with mass spectrometry (AP–MS) and proximity-dependent biotinylation identification (BioID) methods have made...
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Expression, purification and investigation of antibacterial activity of a novel hybrid peptide LL37/hBD-129 by applied comprehensive computational and experimental approaches
Antibiotic-resistant pathogens have become a great universal health concern. Antimicrobial peptides (AMPs) are small amphipathic and cationic...
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Expression and Purification of the Type II Metacaspase from a Unicellular Green Alga Chlamydomonas reinhardtii
Type II metacaspases (MCAs) are proteases, belonging to the C14B MEROPS family. Like the MCAs of type I and type III, they preferentially cleave...
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Phase separation properties of RPA combine high-affinity ssDNA binding with dynamic condensate functions at telomeres
RPA has been shown to protect single-stranded DNA (ssDNA) intermediates from instability and breakage. RPA binds ssDNA with sub-nanomolar affinity,...
