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Real-Time Portable LAMP Assay for a Rapid Detection of Xylella fastidiosa In-Field
Early diagnosis is part of a decision-making process which in the case of plant diseases may prevent the spread of invasive plant pathogens and...
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Assessing the effect of sample storage time on viral detection using a rapid and cost-effective CTAB-based extraction method
BackgroundCassava leaf samples degrade quickly during storage and transportation from distant areas. Proper sampling and efficient, low-cost storage...
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Rapid vertical flow technique for the highly sensitive detection of Brucella antibodies with Prussian blue nanoparticle labeling and nanozyme-catalyzed signal amplification
Brucellosis is a chronic infectious disease caused by Brucella , which is characterized by inflammation of reproductive organs and fetal membranes,...
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Can allele-specific loop-mediated isothermal amplification be used for rapid detection of target-site herbicide resistance in Lolium spp.?
BackgroundHerbicide resistance is one of the threats to modern agriculture and its early detection is one of the most effective components for...
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Rapid and Specific Detection of B. melitensis Targeting BMEI1661 Gene Using Loop-mediated Isothermal Amplification (LAMP) Combined With Lateral Flow immunoassay (LFIA)
B. melitensis is the most pathogenic zoonotic species of Brucella transmitted to animals through fetal secretions, placenta, and vaginal discharges...
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Establishment and application of ERA-LFD method for rapid detection of feline calicivirus
AbstractFeline calicivirus (FCV) has a single-stranded, positive-sense RNA genome, and it is responsible for many infectious respiratory diseases in...
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Recombinase polymerase amplification combined with lateral flow dipstick for the rapid detection of Chattonella marina
Chattonella marina (Raphidophyceae) is a common microalgal species that has frequently caused harmful algal blooms (HABs). Here, recombinase...
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Deep learning and single-cell phenoty** for rapid antimicrobial susceptibility detection in Escherichia coli
The rise of antimicrobial resistance (AMR) is one of the greatest public health challenges, already causing up to 1.2 million deaths annually and...
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Rapid and sensitive detection of genome contamination at scale with FCS-GX
Assembled genome sequences are being generated at an exponential rate. Here we present FCS-GX, part of NCBI’s Foreign Contamination Screen (FCS) tool...
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Development of Simplified Recombinase Polymerase Amplification Assay for Rapid and Robust Detection of Citrus Yellow Vein Clearing Virus
Citrus is an economically important fruit crop, belongs to family Rutaceae, cultivated commercially in over 130 countries, which holds a leading...
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Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viruses infecting patchouli (Pogostemon cablin)
Patchouli ( Pogostemon cablin ), a highly valued medicinal plant, suffers significant economic losses following infection with Broad bean wilt virus 2 ...
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Prevalence of S. aureus and/or MRSA in hospitalized patients with diabetic foot and establishment of LAMP methods for rapid detection of the SCCmec gene
BackgroundPatients with diabetic feet are prone to be infected due to the impaired immune system. However, the prognostic outcome of different...
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Prevalence of bacterial infections and the use of multiplex PCR assay for rapid detection of pathogens in cultured fish in Ghana
The modern and rapid avenue for detecting pathogens provided by molecular genetic techniques including polymerase chain reaction (PCR) was explored...
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Development of the Visual Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD) Method for Rapid Detection of Vibrio harveyi in Aquatic Animals
AbstractVibrio harveyi is a normal flora present in seawater, but in recent years, with large density aquaculture, V. harveyi has become one of the...
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A Cas12a-based fluorescent microfluidic system for rapid on-site human papillomavirus diagnostics
AbstractPersistent infection with human papillomavirus (HPV) is the leading cause of cervical cancer, and early diagnosis is crucial for clinical...
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Recombinase-aided amplification combined with lateral flow dipstick for the rapid detection of Amphidinium carterae
In the context of global climate change, the frequency and duration of harmful algal blooms (HABs) due to eutrophic coastal waters have increased....
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Rapid quantification of miRNAs using dynamic FRET-FISH
MicroRNAs (miRNAs) are short regulatory RNAs that control gene expression at the post-transcriptional level. Various miRNAs playing important roles...
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HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies
AbstractAfrican swine fever (ASF), which is caused by the ASF virus (ASFV), is a highly contagious hemorrhagic disease that causes high mortality to...
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Rapid, sensitive, and specific detection of SARS-CoV-2 in nasopharyngeal swab samples of suspected patients using a novel one-step loop-mediated isothermal amplification (one-step LAMP) technique
BackgroundIn the absence of effective antiviral drugs or vaccines, early and accurate detection of SARS-CoV-2 infection is essential to the COVID-19...
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Establishment of PCR Assay with Internal Amplification Control for Rapid Detection of Salmonella sp.
AbstractSalmonella sp. specific gene fragment 3335471 was used to establish a PCR-based assay for rapid detection of Salmonella sp. To establish the...
