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The DNA Replication, Repair, and Recombination Pathway Genes Modulating Yield and Stress Tolerance Traits in Chickpea
DNA replication, repair, and recombination (DRRR) are the fundamental processes required for faithful transmission of genetic information within and...
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Exonuclease editor promotes precision of gene editing in mammalian cells
BackgroundMany efforts have been made to improve the precision of Cas9-mediated gene editing through increasing knock-in efficiency and decreasing...
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Evaluation of methods to detect circular RNAs from single-end RNA-sequencing data
BackgroundCircular RNA (circRNA), a class of RNA molecule with a loop structure, has recently attracted researchers due to its diverse biological...
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CRISPR/Cas9 system is a suitable gene targeting editing tool to filamentous fungus Monascus pilosus
AbstractMonascus pilosus has been used to produce lipid-lowering drugs rich in monacolin K (MK) for a long period. Genome mining reveals there are...
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Genome Editing in Therapy of Genodermatoses
AbstractThis review is devoted to the prospects for the use of fundamentally important approaches and methods for the correction and therapy of...
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ZGRF1 promotes end resection of DNA homologous recombination via forming complex with BRCA1/EXO1
To maintain genomic stability, the mammalian cells has evolved a coordinated response to DNA damage, including activation of DNA repair and cell...
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Genome editing of Corynebacterium glutamicum mediated with Cpf1 plus Ku/LigD
ObjectivesCorynebacterium glutamicum ( C . glutamicum ) has been harnessed for multi-million-ton scale production of glutamate and lysine. To further...
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Xrcc5/Ku80 is required for the repair of DNA damage in fully grown meiotically arrested mammalian oocytes
Mammalian oocytes spend most of their life in a unique state of cell cycle arrest at meiotic prophase I, during which time they are exposed to...
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Reparaturwege in Krebszellen gezielt ausschalten
Mutations in BRCA1 or BRCA2 are associated with breast, ovarian and other cancers. Cells with these mutations are defective in homologous...
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ABRAXAS1 orchestrates BRCA1 activities to counter genome destabilizing repair pathways—lessons from breast cancer patients
It has been well-established that mutations in BRCA1 and BRCA2 , compromising functions in DNA double-strand break repair (DSBR), confer hereditary...
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C-to-G editing generates double-strand breaks causing deletion, transversion and translocation
Base editors (BEs) introduce base substitutions without double-strand DNA cleavage. Besides precise substitutions, BEs generate low-frequency...
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Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in...
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Origin of the genome editing systems: application for crop improvement
In the last years, genome editing technologies led to a new era of genome engineering, enabling an effective, precise, and rapid engineering of the...
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Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing
Recent advances in genome editing, especially CRISPR-Cas nucleases, have revolutionized both laboratory research and clinical therapeutics....
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Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens
Here, we report the development of methodologies that enable genetic modification of a Basidiomycota yeast, Naganishia liquifaciens . The gene...
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DNA polymerases in precise and predictable CRISPR/Cas9-mediated chromosomal rearrangements
BackgroundRecent studies have shown that, owning to its cohesive cleavage, Cas9-mediated CRISPR gene editing outcomes at junctions of chromosomal...
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Introduction to Phylogenetic Analysis of Molecular Sequence Data
Phylogeny is a model of the relationships between organisms, genes, proteins, or other structures based on common ancestry. It is also used for...
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C9orf72 functions in the nucleus to regulate DNA damage repair
The hexanucleotide GGGGCC repeat expansion in the intronic region of C9orf72 is the most common cause of Amyotrophic lateral sclerosis (ALS) and...
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Improved detection of clinically relevant fusion transcripts in cancer by machine learning classification
BackgroundGenomic rearrangements in cancer cells can create fusion genes that encode chimeric proteins or alter the expression of coding and...
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A genetic tool to express long fungal biosynthetic genes
BackgroundSecondary metabolites (SMs) from mushroom-forming fungi ( Basidiomycota ) and early diverging fungi (EDF) such as Mucoromycota are scarcely...
