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Localization of Long Noncoding RNA in Formalin-Fixed, Paraffin-Embedded Vascular Tissue Using In Situ Hybridization
In situ hybridization (ISH) is a technique for the detection of the location of RNA within a tissue of interest. This process uses oligonucleotides...
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CRISPR-FISH: A CRISPR/Cas9-Based In Situ Labeling Method
Fluorescence in situ hybridization (FISH) has been widely used to visualize target DNA sequences in fixed chromosome samples by denaturing the dsDNA...
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Fluorescence In Situ Hybridization of Uncultured Zoosporic Fungi
Molecular environmental surveys of the eukaryotic microbial community in aquatic ecosystems have revealed a high diversity of sequences belonging to...
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Whole-Mount RNA, Single-Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos
Fluorescence in situ hybridization (FISH) provides a valuable tool for studying the spatial localization of and expression level of genes and cell...
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Optimizing the hybridization chain reaction-fluorescence in situ hybridization (HCR-FISH) protocol for detection of microbes in sediments
Fluorescence in situ hybridization (FISH) is a canonical tool commonly used in environmental microbiology research to visualize targeted cells....
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Multiplex DNA fluorescence in situ hybridization to analyze maternal vs. paternal C. elegans chromosomes
Recent advances in microscopy have enabled studying chromosome organization at the single-molecule level, yet little is known about inherited...
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An Introduction to Fluorescence in situ Hybridization in Microorganisms
Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of...
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Simultaneous In Situ Detection of m6A-Modified and Unmodified RNAs Using DART-FISH
N6-methyladenosine (m6A) is an abundant mRNA modification which plays important roles in regulating RNA function and gene expression. Traditional...
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Visualization and Quantification of Subcellular RNA Localization Using Single-Molecule RNA Fluorescence In Situ Hybridization
Advancements in imaging technologies, especially approaches that allow the imaging of single RNA molecules, have opened new avenues to understand RNA...
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Cytogenetic analysis reveals a mechanism of compatibility in distant hybridization between tree peony and herbaceous peony
Cross-incompatibility is the major challenge in inter-sectional distant hybridization between tree peonies ( Paeonia sect. Moutan ) and herbaceous...
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Super-resolution Chromatin Visualization Using a Combined Method of Fluorescence In Situ Hybridization and Structured Illumination Microscopy in Solanum lycopersicum
Chromatin organization influences gene and transposon expression, and regulates various cellular processes. Higher order chromatin structure has been...
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Experimental Interspecific Hybrids in Nicotiana and Barriers to Hybridization
The history of interspecific hybridization in Nicotiana is reviewed, starting with the work of Koelreuter who in the eighteenth century produced a...
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Fluorescent In Situ Detection of Small RNAs in Plants Using sRNA-FISH
Plant small RNAs are 21–24 nucleotide, noncoding RNAs that function as regulators in plant growth and development. Colorimetric detection of plant...
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In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis
The Porifera are one of the best candidates as the sister group to all other metazoans. Studies on this phylum are therefore expected to shed light...
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In Situ Hybridization Protocols
This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by...
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Automated Cell Counting of Macrophages In Situ
Using the open-source image analysis software CellProfiler to automatically quantify antibody-stained or fluorescently labeled macrophages in situ...
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Chromosome identification in oil palm (Elaeis guineensis) using in situ hybridization with massive pools of single copy oligonucleotides and transferability across Arecaceae species
Chromosome identification is essential for linking sequence and chromosomal maps, verifying sequence assemblies, showing structural variations and...
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Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization
Traditionally, RNA and DNA probes are used in fluorescence in situ hybridization (FISH) methods for microbial detection and characterization of...
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An outlook on fluorescent in situ hybridization coupled to flow cytometry as a versatile technique to evaluate the effects of foods and dietary interventions on gut microbiota
The increasing interest in the effects of the gut microbiota on host health has stimulated the investigation of the composition of this microbial...
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Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization
The RNA in situ hybridization assay is essential in many studies to evaluate gene expression in vivo. It consists of generating tissue sections and...