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Environmental effects on faecal genoty** success in mesocarnivores
Non-invasive genetic sampling can be used in research, monitoring, and conservation of wild animals to, for example, provide insights into diets,...
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Integrated analysis of DNA methylome and transcriptome revealing epigenetic regulation of CRIR1-promoted cold tolerance
BackgroundDNA methylation contributes to the epigenetic regulation of nuclear gene expression, and is associated with plant growth, development, and...
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Getting the chronological age out of DNA: using insights of age-dependent DNA methylation for forensic DNA applications
BackgroundDNA analysis for forensic investigations has a long tradition with important developments and optimizations since its first application....
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DNA Barcoding for an Undergraduate Class
DNA technique is a topic mandatorily covered in a biology and biochemistry undergraduate curriculum. Inquiry-based pedagogy is proven to be the most...
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When conventional methods fall short: identification of invasive cryptic Golden Apple Snails (Pomacea canaliculata; P. maculata) using environmental DNA
Cryptic invasions are difficult to distinguish and easily overlooked by conventional identification methods, creating false biodiversity information....
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Development of a species-specific qPCR assay for the detection of invasive African sharptooth catfish (Clarias gariepinus) using environmental DNA
Detection and monitoring of target species is the primary strategy in the management and control of biological invasions. Traditional methods to...
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Detection of Mycobacterium leprae DNA in clinical and environmental samples using serological analysis and PCR
BackgroundLeprosy is a chronic infectious disease caused by Mycobacterium leprae and persists as a serious public health problem in Brazil. This...
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Insecticide activity under changing environmental conditions: a meta-analysis
The effect of environmental change on activity of insecticides against insects has been greatly debated, and it is of significance to evaluate...
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TA Cloning Approaches to Cloning DNA with Damaged Ends DNA
DNA ends can become damaged for various reasons making them unsuitable for TA cloning techniques, the easiest and most common of the DNA cloning...
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A Pregnancy and Childhood Epigenetics Consortium (PACE) meta-analysis highlights potential relationships between birth order and neonatal blood DNA methylation
Higher birth order is associated with altered risk of many disease states. Changes in placentation and exposures to in utero growth factors with...
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DNA-Based Analysis of Plant Material in Forensic Investigations
Plant material has been used in traditional forensics to reveal and support investigative leads and establish the geographic origin. Identification...
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Applications of Environmental DNA (eDNA) in Monitoring the Endangered Status and Evaluating the Stock Enhancement Effect of Tropical Sea Cucumber Holothuria Scabra
The tropical sea cucumber Holothuria scabra is naturally found in the Indo-West Pacific. However, due to their commercial value, natural H. scabra ...
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Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids
The increasing cultivation and use of genetically modified (GM) crops has increased the importance of environmental monitoring for safety management. B...
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DNA Barcoding and Metabarcoding Protocols for Species Identification
The article presents the several steps to be performed on a plant, fungal, insect, or soil sample to obtain DNA sequences for DNA barcode analysis....
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Progress in the analysis of english channel loliginid squid diets using DNA-metabarcoding techniques
Study of the diet of squid is hampered by the fact that these animals masticate their prey prior to ingestion and do not necessarily ingest the hard...
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DNA methylation and lipid metabolism are involved in GA-induced maize aleurone layers PCD as revealed by transcriptome analysis
BackgroundThe aleurone layer is a part of many plant seeds, and during seed germination, aleurone cells undergo PCD, which is promoted by GA from the...
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Microbial Metagenomics Developments for Environmental and Public Health Monitoring
Microbial metagenomics has transformed surveillance in the fields of community health and the environment by providing previously unobtainable...
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Guidelines for the Analysis of DNA Barcoding/Metabarcoding Sequencing Data and Interpretation of Publicly Available Databases
This chapter describes procedures for the use of DNA sequence data to obtain and compare taxonomic identification using the public databases GenBank...
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A Glossary of DNA Barcoding Terms
This chapter provides a reference glossary for the protocols in this volume. We have chosen only the very basic terms in the DNA barcode lexicon to...
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A Kernelized Classification Approach for Cancer Recognition Using Markovian Analysis of DNA Structure Patterns as Feature Mining
Nucleotide-based molecules called DNA and RNA are essential for several biological processes that affect both normal and cancerous cells. They...
