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Biochemical characterization and cleavage pattern analysis of a novel chitosanase with cellulase activity
Chitosanases are critical tools for the preparation of active oligosaccharides, whose composition is related to the cleavage pattern of the enzyme....
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Cut site preference allows influenza A virus PA-X to discriminate between host and viral mRNAs
Many viruses block host gene expression to take over the infected cell. This process, termed host shutoff, is thought to promote viral replication by...
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Generation of RNA with 2′, 3′-Cyclic Phosphates by Deoxyribozyme Cleavage in Frozen Solutions
The generation of terminal 2′, 3′-cyclic phosphates on RNA oligomers is an important process in the study of tRNA splicing and repair, ribozyme...
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Restriction endonuclease cleavage of phage DNA enables resuscitation from Cas13-induced bacterial dormancy
Type VI CRISPR systems protect against phage infection using the RNA-guided nuclease Cas13 to recognize viral messenger RNA. Upon target recognition,...
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Target cleavage map** and tissue-specific expression analysis of PGPR responsive miR166 under abiotic stress in chickpea (Cicer arietinum L.)
Legumes are an indispensable food after cereals with extensive production across the world. Legume production is imposed with limitations and has...
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dCas9-based gene editing for cleavage-free genomic knock-in of long sequences
Gene editing is a powerful tool for genome and cell engineering. Exemplified by CRISPR–Cas, gene editing could cause DNA damage and trigger DNA...
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Functional surface expression of immunoglobulin cleavage systems in a candidate Mycoplasma vaccine chassis
The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a candidate ‘virulence factor present in multiple pathogenic species of the Mollicutes...
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Structural basis of CHMP2A–CHMP3 ESCRT-III polymer assembly and membrane cleavage
The endosomal sorting complex required for transport (ESCRT) is a highly conserved protein machinery that drives a divers set of physiological and...
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Monitoring eIF4E-Dependent Nuclear 3′ End mRNA Cleavage
In eukaryotes, the maturation of the 3′ ends of most transcripts involves cleavage and polyadenylation steps in the nucleus. While I was working in...
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A de novo binuclear zinc enzyme with DNA cleavage activity
AbstractMetallohydrolases are broadly used throughout biology, often to catalyze the degradation of macromolecules such as DNA and proteins. Many of...
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In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency
The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) system has become a popular toolkit for...
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ReCGBM: a gradient boosting-based method for predicting human dicer cleavage sites
BackgroundHuman dicer is an enzyme that cleaves pre-miRNAs into miRNAs. Several models have been developed to predict human dicer cleavage sites,...
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In vivo cloning of PCR product via site-specific recombination in Escherichia coli
AbstractOver the past years, several methods have been developed for gene cloning. Choosing a cloning strategy depends on various factors, among...
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MMP7 cleavage of amino-terminal CD95 death receptor switches signaling toward non-apoptotic pathways
CD95 is a death receptor that can promote oncogenesis through molecular mechanisms that are not fully elucidated. Although the mature CD95 membrane...
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A Cas12a-based fluorescent microfluidic system for rapid on-site human papillomavirus diagnostics
AbstractPersistent infection with human papillomavirus (HPV) is the leading cause of cervical cancer, and early diagnosis is crucial for clinical...
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CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes
Site-directed and saturation mutagenesis are critical DNA methodologies for studying protein structure and function. For plasmid-based gene mutation,...
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Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in...
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Intradiol ring cleavage dioxygenases from herbivorous spider mites as a new detoxification enzyme family in animals
BackgroundGeneralist herbivores such as the two-spotted spider mite Tetranychus urticae thrive on a wide variety of plants and can rapidly adapt to...
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Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors
BackgroundConventional methods applied to develop recombinant CHO (rCHO) cell line as a predominant host for mammalian protein expression are limited...
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Smartphone Interface and Wearable Biosensors for on-Site Diagnosis
With the rapid development of next-generation manufacturing, communication, and display technologies, smartphone has been widely integrated with...