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ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ
RNA labeling in situ has enormous potential to visualize transcripts and quantify their levels in single cells, but it remains challenging to produce...
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Development and evaluation of a test strip for the rapid detection of antibody against equine infectious anemia virus
AbstractEquine infectious anemia (EIA) is a contagious disease of horses caused by the equine infectious anemia virus (EIAV). The clinical signs at...
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Rapid Detection of Strawberry Mild Yellow Edge Virus with a Lateral Flow Strip Reverse Transcription Recombinase Polymerase Amplification Assay
Strawberry mild yellow edge virus (SMYEV) is a latent virus that severely affects the yield and quality of strawberry fruit. The technology suitable...
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Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Hemileia vastatrix in coffee plantations
Coffee leaf rust (CLR) caused by Hemileia vastatrix is a devastating worldwide disease. Early monitoring is crucial for controlling CLR quickly and...
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Mitochondrial genome provides species-specific targets for the rapid detection of early invasive populations of Hylurgus ligniperda in China
BackgroundHylurgus ligniperda , a major international forestry quarantine pest, was recently found to have invaded and posed a serious threat to the Pinus...
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Development and evaluation of rapid and accurate one-tube RPA-CRISPR-Cas12b-based detection of mcr-1 and tet(X4)
AbstractThe emergence and quick spread of the plasmid-mediated tigecycline resistance gene tet (X4) and colistin resistance gene mcr-1 have posed a...
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Rapid detection of porcine circovirus type 2 by a red latex microsphere immunochromatographic strip
AbstractTo establish a rapid and specific antigen detection method for porcine circovirus type 2 (PCV2), monoclonal antibodies (mAbs) were produced...
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Four thermostatic steps: A novel CRISPR-Cas12-based system for the rapid at-home detection of respiratory pathogens
AbstractThe outbreak of coronavirus disease 2019 (COVID-19) in 2019 has severely damaged the world's economy and public health and made people pay...
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MALDI-TOF MS, Schnelltests und Rapid microbiological detection systems
Eine noch neuere Methode (verglichen mit der PCR) ist die Identifizierung von Bakterien mittels Massenspektrometrie. Die Methode wird als MALDI-TOF... -
Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes
BackgroundS. pyogenes , is a primary pathogen that leads to pharyngitis and can also trigger severe conditions like necrotizing fasciitis and...
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Effect of food matrix on rapid detection of Vibrio parahaemolyticus in aquatic products based on toxR gene
Vibrio parahaemolyticus has become an important public threat to human health. Rapid and robust pathogen diagnostics are necessary for monitoring its...
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Evaluation of Monochromatic Excitation X-ray Fluorescence Spectrometry for Rapid Thallium Detection in Biological Samples Using Animal Models
Monochromatic excitation X-ray fluorescence (ME-XRF) spectrometry is a novel technique for trace element analysis, characterized by its simplicity,...
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Rapid detection and identification of fungi in grain crops using colloidal Au nanoparticles based on surface-enhanced Raman scattering and multivariate statistical analysis
Grain crops are easily contaminated by fungi due to the existence of various microorganisms in the storage process, especially in humid and warm...
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Sensitive and rapid detection of tet(X2) ~ tet(X5) by loop-mediated isothermal amplification based on visual OTG dye
The emergence of tigecycline-resistant tet(X2/X3/X4/X5) genes poses a new threat to the efficacy of anti-infective therapy and the safety of our food...
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Revolutionizing food safety with electrochemical biosensors for rapid and portable pathogen detection
Foodborne diseases remain a worldwide concern, despite the advances made in sanitation, pathogen surveillance and food safety management systems. The...
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Application of the prenatal BACs-on-Beads™ assay for rapid prenatal detection of sex chromosome mosaicism
The prenatal BACs-on-Beads™ (BoBs) assay was introduced for rapid detection of abnormalities of chromosomes 13, 18, 21, X, and Y and specific nine...
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Development of a rapid, accurate, and field deployable LAMP-CRISPR-Cas12a integrated assay for Xylella fastidiosa detection and surveillance
Xylella fastidiosa is an aggressive plant pathogenic bacterium of significant quarantine concern. Accurate and reliable detection tools are essential...
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Development and application of a rapid detection method for Clarireedia spp. using loop-mediated isothermal amplification (LAMP) assay
Dollar spot, caused by the ascomycete fungus, Clarireedia spp., is a disease that seriously damages most grass species, especially those grown on...
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Rapid and simultaneous detection of multiple pathogens in the lower reproductive tract during pregnancy based on loop-mediated isothermal amplification-microfluidic chip
BackgroundFemale reproductive tract infection (RTI) is the common source of varied diseases, especially as an important risk factor for pregnancy...