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Establishment and optimization of PEG-mediated protoplast transformation in the microalga Haematococcus pluvialis
Genetic manipulation of Haematococcus pluvialis is difficult because of the lack of a stable and convenient transformation system. The pH124-EGFP-Ble...
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Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis
Protoplasts are an attractive explant source for biotechnological tools widely used on citrus genetic improvement, such as somatic hybridization and...
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Isolation of Adhirons Specific for Plant Protoplast Membrane Biomarkers Is Simplified by Phagemid Design
Phage display is an effective method to retrieve binders specific for a target epitope from a large clone library. Nevertheless, the panning process...
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Tissue Culture and Somatic Fusion in Plants
Molecular biology is a great avenue to combat climatic risks and challenges in agriculture. Interventions have improved the seasonal fluctuation in...
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Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses
Forage and turf grasses are widely grown and contribute significantly to sustainable agriculture. This chapter describes a protocol for protoplast...
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A highly efficient stratagem for protoplast isolation and genetic transformation in filamentous fungus Colletotrichum falcatum
Red rot of sugarcane caused by the hemi-biotrophic fungal pathogen, Colletotrichum falcatum , is a major threat to sugarcane cultivation in many...
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Optimization of protoplast regeneration in the model plant Arabidopsis thaliana
BackgroundPlants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the...
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Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration
Insertion of a specific sequence in a targeted region for precise editing is still a major challenge in plants. Current protocols rely on inefficient...
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Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis Via Protoplast Transfection
Recent advances in DNA synthesis and assembly allow for genetic constructs to be designed and constructed in high throughput. Characterizing large...
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Efficient production of transgene-free, gene-edited carrot plants via protoplast transformation
Key messageWe have developed and validated an efficient protocol for producing gene-edited carrot plants that do not result in the stable...
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CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration
Clustered regularly interspaced short palindromic repeats (CRISPRCRISPR)-Cas (CRISPR-associated system) has become the multipurpose tool to...
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Tissue Culture Techniques for Saffron Improvement
Several clonal propagation methods are able to produce true-to-type clones of saffron plants under aseptic controlled conditions. Saffron in vitro...
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Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense ) and Camelina (Camelina sativa )
Pennycress (Thlaspi arvense) and camelina (Camelina sativa) are nonfood winter oilseed crops that have the potential to contribute to sustainable...
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In Vitro Culture of Arabidopsis Embryos
Arabidopsis thaliana is currently the most important model organism for basic molecular plant research. It is also a favourable model for...
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Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System
Protein stability influences many aspects of biology, and measuring their stability in vivo can provide important insights into biological systems....
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Establishing an efficient protoplast transient expression system for investigation of floral thermogenesis in aroids
Key messageFloral thermogenesis is an important reproductive strategy for attracting pollinators. We developed essential biological tools for...
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Interspecific hybridization between Ganoderma lingzhi and G. applanatum through protoplast fusion
Interspecific hybridization between Ganoderma lingzhi and G. applanatum was attempted through polyethylene glycol (PEG) induced fusion technique. The...
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Plant tissue culture-mediated biotechnological approaches in Lycium barbarum L. (Red goji or wolfberry)
Plant tissue culture has long evolved since its first successful attempt by Gottlieb Haberlandt in 1902, and has then emerged as a powerful...
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Protoplast fusion between Blakeslea trispora 14,271 (+) and 14,272 (−) enhanced the yield of lycopene and β-carotene
Blakeslea trispora , a heterothallic Zygomycota with two mating types (termed “plus” and “minus”), is an ideal source of lycopene and β-carotene. The...
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Enhanced polyethylene glycol (PEG)–mediated protoplast transformation system for the phytopathogenic fungus, Ganoderma boninense
The basidiomycete fungus, Ganoderma boninense , has been identified as the main causal agent of oil palm basal stem rot (BSR) disease which has caused...
