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Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies
Spurred by advances in AI-driven modeling and experimental methods, molecular dynamics simulations are now acting as a platform to integrate these...
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Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein–LPS Interactions
Site-directed spin labeling EPR (electron paramagnetic resonance) spectroscopy is a technique used to identify the local conformational changes at a...
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Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry
Chemical crosslinking-mass spectrometry (XL-MS) is an established tool that can be used to study the architecture and dynamics of proteins and...
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Stress-Based Screening for Compounds That Inhibit β-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria
Biogenesis of the outer membrane (OM) of Gram-negative bacteria involves two processes essential for growth, that is, the insertion of β-barrel outer...
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Ligand-bound glutamine binding protein assumes multiple metastable binding sites with different binding affinities
Protein dynamics plays key roles in ligand binding. However, the microscopic description of conformational dynamics-coupled ligand binding remains a...
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Identification of metal binding motifs in protein frameworks to develop novel remediation strategies for Hg2+ and Cr(VI)
Amino acid sequences in metal-binding proteins with chelating properties offer exciting applications in biotechnology and medical research. To...
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Computational identification of putative copper-binding proteins in pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae
Xanthomonas citri pv. punicae ( Xcp ) causes bacterial blight in pomegranate, and an effective management strategy to control this devastating disease...
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Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella
Polysaccharides play critical roles in bacteria, including the formation of protective capsules and biofilms and establishing specific host cell...
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Mycobacterium tuberculosis senses host Interferon-γ via the membrane protein MmpL10
Mycobacterium tuberculosis (Mtb) is one of the most successful human pathogens. Several cytokines are known to increase virulence of bacterial...
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A Bacterial Two-Hybrid System for In Vivo Assays of Protein-Protein Interactions and Drug Discovery
The bacterial adenylate cyclase-based two-hybrid (BACTH) system is a robust and simple genetic assay used to monitor protein-protein interactions in...
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Mechanisms Underlying the Virulence and Intestinal Colonization of the Vibrio alginolyticus HY9901 DctP Protein with Proteomic Analysis
AbstractVibrio alginolyticus as a common pathogen infecting humans and marine animals has become a severe threat to the global mariculture industry....
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Binding Efficacy of Heavy Chain Antibody Against Porcine Alpha Amylase Derived from Indian Camel (Camelus dromedarius)
Single domain antibody has huge application in the field of biological sciences. Though many studies have been done on camelid antibody generated...
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Quorum sensing in bacteria: in silico protein analysis, ecophysiology, and reconstruction of their evolutionary history
BackgroundQuorum sensing (QS) is a sophisticated cell-to-cell signalling mechanism that allows the coordination of important processes in microbial...
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Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production
Despite the importance of recombinant protein production in the academy and industrial fields, many issues concerning the expression of soluble and...
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Affinity Purification and Coimmunoprecipitation of Transenvelope Protein Complexes in Gram-Negative Bacteria
Multiprotein complexes are important machineries that organize a large number of different proteins into functional units. Studying protein-protein...
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Identification and characterisation of a major outer membrane protein from Methylacidiphilum fumariolicum SolV
The outer membrane (OM) protects Gram-negative bacteria against a hostile environment. The proteins embedded in the OM fulfil a number of tasks that...
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In Vitro Reconstruction of Bacterial β-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane
The in vitro reconstruction assay enables us to evaluate in detail the insertion and proper protein folding (together termed assembly) of β-barrel...
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Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP
BackgroundGlutamate and aspartate are preferred nutrients for a variety of microorganisms. In the case for many Pseudomonas spp., utilization of...
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Bacillus subtilis: current and future modification strategies as a protein secreting factory
Bacillus subtilis is regarded as a promising microbial expression system in bioengineering due to its high stress resistance, nontoxic, low codon...
