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Characterization and biocompatibility evaluation of acellular rat skin scaffolds for skin tissue engineering applications
Utilization of acellular scaffolds, extracellular matrix (ECM) without cell content, is growing in tissue engineering, due to their high...
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MpMLO1 controls sperm discharge in liverwort
In bryophytes, sexual reproduction necessitates the release of motile sperm cells from a gametophyte into the environment. Since 1856, this process,...
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A palmitoylation–depalmitoylation relay spatiotemporally controls GSDMD activation in pyroptosis
Gasdermin D (GSDMD) is the executor of pyroptosis, which is important for host defence against pathogen infection. Following activation,...
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TAF2, within the TFIID complex, regulates the expression of a subset of protein-coding genes
TFIID, one of the general transcription factor (GTF), regulates transcriptional initiation of protein-coding genes through direct binding to promoter...
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Oxygenation of Anandamide by Lipoxygenases
The endocannabinoids anandamide and 2-arachidonoylglycerol are not only metabolized by serine hydrolases, such as fatty acid amide hydrolase,...
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NINJ1 is activated by cell swelling to regulate plasma membrane permeabilization during regulated necrosis
Plasma membrane permeabilization (PMP) is a defining feature of regulated necrosis. It allows the extracellular release of damage-associated...
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Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells
Gene regulation in the nucleus requires precise control of the molecular processes that dictate how, when, and which genes are transcribed. The...
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TMEM63B channel is the osmosensor required for thirst drive of interoceptive neurons
Thirst plays a vital role in the regulation of body fluid homeostasis and if deregulated can be life-threatening. Interoceptive neurons in the...
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Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms
Impairment of protein translation can cause stalling and collision of ribosomes and is a signal for the activation of ribosomal surveillance and...
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Biomimetic Nanosystems in Targeted Drug Delivery
Nanotechnology in cancer has been a boon to the translational science bringing advantages to the conventional drug delivery approaches. There are...
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Chloride Channel-3 (ClC-3) Modifies the Trafficking of Leucine-Rich Repeat-Containing 8A (LRRC8A) Anion Channels
Chloride channel-3 (ClC-3) Cl − /H + antiporters and leucine-rich repeat-containing 8 (LRRC8) family anion channels have both been associated with...
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A New Methanogenic, Hydrogenotrophic Archaeon from Spitsbergen Permafrost
AbstractA new strain of methanogenic archaea, designated VT T , was isolated from a sample of Spitsbergen permafrost. The cells were nonmotile curved...
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Synchronization of Cultured Cells to G1, S, G2, and M Phases by Double Thymidine Block
The typical cell cycle in eukaryotes is composed of four phases including the G1, S, G2, and M phases. G1, S, and G2 together are called interphase....
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Heterologous Expression and Purification of GPCRs
G protein-coupled receptors (GPCRs) are involved in a variety of human physiological processes and are attractive targets for treating various...
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Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells
Immunoaffinity purification allows for the purification of epitope-tagged proteins and their associated multisubunit complexes from mammalian cells....
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Drosophila TMEM63 and mouse TMEM63A are lysosomal mechanosensory ion channels
Cells sense physical forces and convert them into electrical or chemical signals, a process known as mechanotransduction. Whereas extensive studies...
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Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody
The Hedgehog signaling pathway coordinates early development and is important in various cancers. Classic approaches to test pathway activation rely...
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Oncoprotein SET dynamically regulates cellular stress response through nucleocytoplasmic transport in breast cancer
SETβ is the predominant isoform of oncoprotein SE translocation (SET) in various breast cancer cell lines. Interactome-transcriptome analysis has...
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ROS formation, mitochondrial potential and osmotic stability of the lamprey red blood cells: effect of adrenergic stimulation and hypoosmotic stress
Semi-anadromous animals experience salinity fluctuations during their life-span period. Alterations of environmental conditions induce stress...
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Monitoring Telomere Maintenance During Regeneration of Annelids
Telomere shortening is a hallmark of aging and eventually constrains the proliferative capacity of cells. The protocols discussed here are used for...