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Isolation of Cytosolic Ribosomes Associated with Plant Mitochondria and Chloroplasts
Excluding the few dozen proteins encoded by the chloroplast and mitochondrial genomes, the majority of plant cell proteins are synthesized by...
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Cryopreservation of shoot meristems of Baliospermum montanum (Willd.) Muell. Arg. for long-term conservation and sustainable utilization
Long-term in vitro conservation through cryopreservation was successfully established for the first time in Baliospermum montanum (Willd.) Muell....
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Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels
Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8...
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Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2): A Cation Channel Activated by ADP-Ribose and Involved in Cell Death
Transient receptor potential melastatin-2 (TRPM2) is an emerging chemotherapeutic target due to its involvement in poly(ADP-ribose) metabolism and...
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Analysis of mRNA Translation by Polysome Profiling
mRNA translation is a key step in gene expression that allows the cell to qualitatively and quantitatively modulate the cell’s proteome according to...
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Analysis for Sister Chromatid Exchange
Sister chromatid exchange (SCE) is the exchange event of genetic material between two identical sister chromatid. Elevation of SCE frequency is...
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Imaging Flow Cytometry of Multi-Nuclearity
Multi-nuclearity is a common feature for cells in different cancers. Also, analysis of multi-nuclearity in cultured cells is widely used for...
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Isolation of Polystyrene Bead-Induced Phagosomes for Western Blotting
The engulfment of “self” and “non-self” particles by immune and non-immune cells is crucial for maintaining homeostasis and combatting infection....
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SH2-Domain Protein Isolation Using Synthetic Phosphorylated Peptides to Study VEGFR2 Signaling
Vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) signaling pathways are tightly regulated multistep chain reactions that involve a...
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Chromosomal Analysis in Lineage-Specific Mouse Hematopoietic Stem Cells and Progenitors
Hematopoiesis is maintained throughout life from the hematopoietic stem cell niche in which hematopoietic stem cells and lineage-specific...
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An optogenetic method for the controlled release of single molecules
We developed a system for optogenetic release of single molecules in cells. We confined soluble and transmembrane proteins to the Golgi apparatus via...
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A de novo marker chromosome 15 in a child with isolated developmental delay
We report a rare case of a 14-month-old male child who was referred for developmental delay. Clinical examination revealed a hypotonic infant with...
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High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes
Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore...
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Chemical-Induced Premature Chromosome Condensation Protocol
Chromosome analysis is one of most fundamental techniques for cytogenetic studies. Chromosomes are conventionally prepared from mitotic cells...
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Extracellular Vesicles: The Challenges on the Way and Engineering Perspectives
Extracellular vesicles (EVs) are found in every body fluid and are known to be released by every human cell. So far, EVs have been classified into...
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Osmoregulation
Several teleost fish species exhibit spectacular migration between seawater (SW) and freshwater (FW) environments, including the Japanese eel...
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Apoptosis Detection Assays
Many apoptosis assays are available since there are many proteins regulated at multiple points and involved in apoptosis signaling cascade. To detect...
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The Significance of Repressive Processes in Developmental Regulation
Encountering a developmental process confined to a limited time window or a restricted embryonic area, one may deem that the mechanism to activate...
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Effects of temperature and salinity on the larval early development, growth, and settlement of the diploid, triploid, and tetraploid Pacific oyster “Haida No. 2” strain
Tetraploids play a crucial role in the large-scale production of all-triploid Pacific oyster, Crassostrea gigas by mating with diploids. For nursery...
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L-form conversion in Gram-positive bacteria enables escape from phage infection
At the end of a lytic bacteriophage replication cycle in Gram-positive bacteria, peptidoglycan-degrading endolysins that cause explosive cell lysis...
