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Global DNA methylation and cellular 5-methylcytosine and H4 acetylated patterns in primary and secondary dormant seeds of Capsella bursa-pastoris (L.) Medik. (shepherd’s purse)
Despite the importance of dormancy and dormancy cycling for plants’ fitness and life cycle phenology, a comprehensive characterization of the global...
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Embryogenesis, Growth, and Differentiation
An essential feature of plant development is the ability of plant cells to grow and differentiate. Growth of an organism is defined as an... -
Pollen Cryopreservation in Cassava
Crossing between the parental lines is very difficult in cassava since the flowering depends on the genotype and environmental conditions. Poor and... -
A protocol for Agrobacterium-mediated genetic transformation of Lens culinaris Medik (lentil)
A reliable protocol for Agrobacterium -mediated genetic transformation of Lens culinaris Medik (lentil) was developed. Using cultivar Laird, the...
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Genome-wide expression analysis of LACS gene family implies GhLACS25 functional responding to salt stress in cotton
BackgroundLong-chain acyl-coenzyme A synthetase (LACS) is a type of acylating enzyme with AMP-binding, playing an important role in the growth,...
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Gymnosperms from the Early Cretaceous Crato Flora: Competitors for the Nascent Flowering Plants
The Lower Cretaceous (likely upper Barremian to lower Aptian) flora of the Crato Formation in the Araripe Basin in NE Brazil opens a unique window to... -
A cytosol-tethered YHB variant of phytochrome B retains photomorphogenic signaling activity
The red and far-red light photoreceptor phytochrome B (phyB) transmits light signals following cytosol-to-nuclear translocation to regulate...
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Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules
In this chapter, approaches to the image analysis of the choreography of the plant endoplasmic reticulum (ER) labeled with fluorescent fusion... -
Somatic embryogenesis and plantlet regeneration in red sandalwood (Pterocarpus santalinus)
Cotyledonary segments from the germinated immature zygotic embryos were used for the somatic embryogenesis of red sandalwood ( Pterocarpus santalinus )....
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Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions
Plants live in highly dynamic surroundings and need to cope with constant environmental challenges. In order to do so, they developed quick reactions... -
SIAMESE-RELATED1 imposes differentiation of stomatal lineage ground cells into pavement cells
The leaf epidermis represents a multifunctional tissue consisting of trichomes, pavement cells and stomata, the specialized cellular pores of the...
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Afrothismiaceae (Dioscoreales), a new fully mycoheterotrophic family endemic to tropical Africa
Afrothismia is a genus of non-photosynthetic mycoheterotrophs from the forests of continental tropical Africa. Multiple phylogenetic inferences using...
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Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies
Polyethersulfone (PES) membranes provide a versatile tool for gravity-related plant studies. Benefits of this system include straightforward setup,... -
High-throughput time series expression profiling of Plasmopara halstedii infecting Helianthus annuus reveals conserved sequence motifs upstream of co-expressed genes
Downy mildew disease of sunflower, caused by the obligate biotrophic oomycete Plasmopara halstedii, can have significant economic impact on sunflower...
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Phylogeny and Circumscription of the Subfamily Olisbeoideae
The Olisbeoideae are a pantropical group comprising six genera and ca. 557 currently recognized species. Members of this subfamily are... -
Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina
BackgroundPennycress and camelina are two important novel biofuel oilseed crop species. Their seeds contain high content of oil that can be easily...
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Cut and paste: temperature-enhanced cotyledon micrografting for Arabidopsis thaliana seedlings
BackgroundCotyledon micrografting represents a useful tool for studying the central role of cotyledons during early plant development, especially...
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RNAi Expression in Cotton for Control of Herbivorous Insects
Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests... -
Somatic embryogenesis in Abies nebrodensis, an endangered Sicilian fir
Somatic embryogenesis, as a promising biotechnological tool for many conifer trees, has never been applied for the Abies nebrodensis species....