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Virome analysis of sweetpotato in three Brazilian regions using high-throughput sequencing
Sweetpotato ( Ipomoea batatas (L.) Lam.) is a highly nutritious food crop that suffers severe yield losses due to viral diseases. These are...
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3D-GBS: a universal genoty**-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes
Despite the increased efficiency of sequencing technologies and the development of reduced-representation sequencing (RRS) approaches allowing...
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Genetic Screening using High-Throughput Barcode Sequencing of Saccharomyces cerevisiae Knockout Collection: Search for Regulators of mtDNA Intracellular Selection
AbstractWe searched for genes whose deletion affects mitochondrial heteroplasmy in the yeast Saccharomyces cerevisiae . In order to do this, we...
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Highly accurate species identification of Eastern Pacific rockfishes (Sebastes spp.) with high-throughput DNA sequencing
Genetic species identification is often necessary for species flocks, such as rockfishes in the genus Sebastes (Teleostei, Scorpaenidae). Traditional...
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The 16S rDNA high-throughput sequencing correlation analysis of milk and gut microbial communities in mastitis Holstein cows
This study aimed to understand the changes in the milk and gut microbiota of dairy cows with mastitis, and to further explore the relationship...
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Analysis of Endophytic Bacterial Diversity in Rice Seeds with Regional Characteristics in Yunnan Province, China, Based on High-Throughput Sequencing Technology
Examining the endophytic bacteria in rice seeds from Yunnan Province displaying regional characteristics is vital for exploring strain resources,...
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DNA Fragment Enrichment for High-Throughput Sequencing
AbstractThis review describes the application of oligonucleotides, which are mainly obtained using DNA synthesizers of a new generation (microarray...
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Unraveling MicroRNA Mediated Gene Regulation in Centella asiatica (L.) Urb. by high-Throughput Sequencing Based Small-RNA Profiling
Centella asiatica is a widely spread herb mostly found in the tropics having extensive medicinal values. Here, we report for the first time,...
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Diversity of heterotrophic picoeukaryotes in the ice of the Kandalaksha Gulf (White Sea, Russia) based on rRNA gene high-throughput sequencing
Contemporary climate change in the Arctic is causing the reduction of the ice habitat. This process induces rearrangements in the community...
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Applications of High-Throughput DNA Sequencing to Single-Domain Antibody Discovery and Engineering
Next-generation DNA sequencing (NGS) technologies have made it possible to interrogate antibody repertoires to unprecedented depths, typically via...
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Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products
Polymerase Chain Reaction (PCR) products have been traditionally characterized by cloning and cycle sequencing. However, when quick sequencing data...
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Development of SSR markers in top-mouth culter (Erythroculter ilishaeformis) based on high-throughput sequencing
The top-mouth culter Erythroculter ilishaeformis is a kind of carnivorous fish that is currently a widely distributed fish in China, which is a...
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Deciphering the wheat seed core mycobiome of two Egyptian cultivars (Giza 171 and Sids 14) by using high throughput amplicon sequencing of the ITS2 region
Wheat ( Triticum aestivum L.) is an important food crop throughout the world. The seed mycobiome is the primary inoculum affecting the overall health...
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High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice
BackgroundTo investigate the differences in bacterial and fungal community structure and diversity in conjunctival tissue of healthy and diabetic...
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Epitranscriptome Map** of N6-Methyladenosine Using m6A Immunoprecipitation with High Throughput Sequencing in Skeletal Muscle Stem Cells
N6-Methyladenosine (m6A), one of the most abundant chemical modifications in mRNA (epitranscriptome), contributes to the regulation of biological...
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Construction and functional enrichment analysis of the competitive endogenous RNA regulatory network for nonarteritic anterior ischemic optic neuropathy based on high-throughput sequencing
Based on the transcriptome high-throughput sequencing of nonarteritic anterior ischemic optic neuropathy (NAION), this study constructed a...
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m6A RNA Immunoprecipitation Followed by High-Throughput Sequencing to Map N6-Methyladenosine
N6-methyladenosine (m6A) is the most abundant internal modification on messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) in eukaryotes. It...
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High-throughput sequencing reveals the dominance of Shewanella species in the intestinal microbiota of barbour’s seahorses (Hippocampus barbouri)
In this study, taxonomic classification of intestinal microbiota from healthy Barbour’s seahorses ( Hippocampus barbouri ) was determined as it plays...
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Crop Phenomics and High-Throughput Phenoty**
Abiotic stresses, like drought, salinity, and high temperature pose significant challenges to global agriculture, jeopardizing crop yields and food...