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A novel 3D bilayer hydrogel tri-culture system for studying functional motor units
BackgroundA motor unit (MU) is formed by a single alpha motor neuron (MN) and the muscle fibers it innervates. The MU is essential for all voluntary...
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A protocol for good quality genomic DNA isolation from formalin-fixed paraffin-embedded tissues without using commercial kits
BackgroundDNA isolation from formalin-fixed paraffin-embedded (FFPE) tissues for molecular analysis has become a frequent procedure in cancer...
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PriPath: identifying dysregulated pathways from differential gene expression via grou**, scoring, and modeling with an embedded feature selection approach
BackgroundCell homeostasis relies on the concerted actions of genes, and dysregulated genes can lead to diseases. In living organisms, genes or their...
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A newly developed paper embedded microchip based on LAMP for rapid multiple detections of foodborne pathogens
BackgroundMicrofluidic chip detection technology is considered a potent tool for many bioanalytic applications. Rapid detection of foodborne...
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PbGP43 Genoty** Using Paraffin-Embedded Biopsies of Human Paracoccidioidomycosis Reveals a Genetically Distinct Lineage in the Paracoccidioides brasiliensis Complex
Paracoccidioidomycosis (PCM) is a systemic mycosis caused by a group of cryptic species embedded in the Paracoccidioides brasiliensis complex and Parac...
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Expert System
This chapter is concerned with expert system for forecasting. It comprises of expert system architecture and its characteristic features. The entire... -
Membrane-embedded TSPO: an NMR view
Translocator Protein (18 kDa) (TSPO) is a mitochondrial transmembrane protein commonly used as a biomarker for neuroinflammation and is also a...
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Efficient and rapid system of plant regeneration via protoplast cultures of Fagopyrum esculentum Moench
In the present study, a high yield of isolated protoplasts from the agronomically important crop Fagopyrum esculentum was obtained by applying a...
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Theoretical elucidation of the structure, bonding, and reactivity of the CaMn4Ox clusters in the whole Kok cycle for water oxidation embedded in the oxygen evolving center of photosystem II. New molecular and quantum insights into the mechanism of the O–O bond formation
This paper reviews our historical developments of broken-symmetry (BS) and beyond BS methods that are applicable for theoretical investigations of...
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Reward system activation improves recovery from acute myocardial infarction
Psychological processes have a crucial role in the recovery from acute myocardial infarction (AMI), yet the underlying mechanisms of these effects...
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Results and lessons from dual extraction of DNA and RNA from formalin-fixed paraffin-embedded breast tumor tissues for a large Cancer epidemiologic study
BackgroundThe use of archived formalin-fixed paraffin-embedded (FFPE) tumor tissues has become a common practice in clinical and epidemiologic...
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Wharton’s jelly mesenchymal stem cells embedded in PF-127 hydrogel plus sodium ascorbyl phosphate combination promote diabetic wound healing in type 2 diabetic rat
BackgroundDiabetic cutaneous ulcers (DCU) are a complication of diabetes with diabetic foot ulcers being the most common, and the wounds are...
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Future opportunities in solar system plasma science through ESA’s exploration programme
The solar wind interacts with all solar system bodies, inducing different types of dynamics depending on their atmospheric and magnetic environments....
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Quantifying Trophoblast Function Using the xCELLigence System
Analyses of trophoblast behavior in vitro are widely used to investigate and understand the trophoblast dysfunction associated with pregnancy... -
First Detection of Podocytes in the Circulatory System of Enigmatic Echiurids (Annelida: Thalassematidae)
AbstractThe fine structure of echiurid blood vessels in the proboscis is known in detail, but the circulatory system of the trunk is still understood...
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Map** of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system
Achieving uniform optical resolution for a large tissue sample is a major challenge for deep imaging. For conventional tissue clearing methods, loss...
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Targeted mutagenesis in mice via an engineered AsCas12f1 system
SpCas9 and AsCas12a are widely utilized as genome editing tools in human cells, but their applications are largely limited by their bulky size....
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Time Transformation in the Sign System of the Conditioned Reflex
How is time transformed when signs appear? In the sign system of the conditioned reflex, the sign (conditioned stimulus) reverses, changes the...
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Incorporating human behaviour into Earth system modelling
Climate change and other challenges to the stability and functioning of natural and managed environmental systems are driven by increasing...
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HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography
Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication of cryo-lamella of frozen native specimens for study by in...