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Display of Streptococcus iniae α-Enolase on the Surface of Virus-Like Particles (VLPs) of Nervous Necrosis Virus (NNV) Using SpyTag/SpyCatcher
Virus-like particle (VLP)-based vaccines are promising candidates for overcoming the safety problems of live vaccines and weak immunogenicity of...
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The RRE–Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles
AbstractDelivery of ribonucleoprotein complexes of Cas9 nuclease and guide RNA into target cells with virus-like particles (VLP) is one of the novel...
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Exogenous loading of extracellular vesicles, virus-like particles, and lentiviral vectors with supercharged proteins
Cell membrane-based biovesicles (BVs) are important candidate drug delivery vehicles and comprise extracellular vesicles, virus-like particles, and...
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The Applications of Artificial Intelligence (AI)-Driven Tools in Virus-Like Particles (VLPs) Research
Viral-like particles (VLPs) represent versatile nanoscale structures mimicking the morphology and antigenic characteristics of viruses, devoid of...
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In vitro assembly of chimeric virus-like particles composed of a porcine circovirus 2b capsid protein and a B-cell epitope of infectious bursal disease virus
ObjectivesTo develop a method for in vitro assembly of recombinant proteins expressed in E. coli into chimeric virus-like particles (cVLPs).
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Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-like Particles
Overexpression of biologically functional GPCRs and homogeneous purified protein solutions are required to enable structural studies and... -
Virus-like particles isolated from reactivated biological soil crusts
A novel method was developed for virus-like particle (VLP) extraction and characterization from biological soil crust (BSC) after microbial community...
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High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler
We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of...
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Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus
Many virus capsids undergo exquisitely choreographed maturation processes in their host cells to produce infectious virions, and these remain poorly...
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Detection of viral antibodies in camel sera using magnetic particle spectroscopy
AbstractPandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of...
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Archaeal Viruses: Production of Virus Particles and Vesicle-like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation
Asymmetrical flow field-flow fractionation (AF4) is a separation method based on hydrodynamic size of the sample components. It can separate a broad... -
Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development
Virus-like particle (VLP) technology is an alternative platform for develo** vaccines to combat seasonal and pandemic influenza. Influenza VLPs are... -
Immunogenicity and protective potency of Norovirus GII.17 virus-like particle-based vaccine
ObjectivesNoroviruses (NoVs) are major cause of acute viral gastroenteritis in worldwide, and the lack of a cell culture system that must be...
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Caspase-8 is required for HSV-1-induced apoptosis and promotes effective viral particle release via autophagy inhibition
Regulated cell death (RCD) plays an important role in the progression of viral replication and particle release in cells infected by herpes simplex...
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Antibodies from dengue patients with prior exposure to Japanese encephalitis virus are broadly neutralizing against Zika virus
Exposure to multiple mosquito-borne flaviviruses within a lifetime is not uncommon; however, how sequential exposures to different flaviviruses shape...
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Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution
In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM)...
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Virusähnliche Partikel – Impfstoffe, die den Eindringling imitieren
Virus-like particle vaccines have emerged as a promising and innovative approach to combat infectious diseases. Despite their immense potential as...
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Breaking the Ice: A Review of Phages in Polar Ecosystems
Bacteriophages, or phages, are viruses that infect and replicate within bacterial hosts, playing a significant role in regulating microbial... -
A plant-based transient expression system for the rapid production of highly immunogenic Hepatitis E virus-like particles
ObjectiveHepatitis E virus (HEV) infection is a major cause of acute hepatitis worldwide. The aim of the study is the development of plant expression...
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Molecular detection of cacao swollen shoot badnavirus species by amplification with four PCR primer pairs, and evidence that Cacao swollen shoot Togo B virus-like isolates are highly prevalent in Côte d’Ivoire
Badnaviruses are plant pararetroviruses that infect economically important crops worldwide, and several badnaviral species have been reported causing...