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  1. Reverse engineering environmental metatranscriptomes clarifies best practices for eukaryotic assembly

    Background

    Diverse communities of microbial eukaryotes in the global ocean provide a variety of essential ecosystem services, from primary production...

    Arianna I. Krinos, Natalie R. Cohen, ... Harriet Alexander in BMC Bioinformatics
    Article Open access 03 March 2023
  2. MitoHiFi: a python pipeline for mitochondrial genome assembly from PacBio high fidelity reads

    Background

    PacBio high fidelity (HiFi) sequencing reads are both long (15–20 kb) and highly accurate (> Q20). Because of these properties, they have...

    Marcela Uliano-Silva, João Gabriel R. N. Ferreira, ... Shane A. McCarthy in BMC Bioinformatics
    Article Open access 18 July 2023
  3. Monomicellar assembly to synthesize structured and functional mesoporous carbonaceous nanomaterials

    The large pores of functional mesoporous carbonaceous nanomaterials have broad accessibility, making them efficient substrates for the mass transport...

    Liang Peng, Huarong Peng, ... Dongyuan Zhao in Nature Protocols
    Article 14 December 2022
  4. Bookend: precise transcript reconstruction with end-guided assembly

    We developed Bookend, a package for transcript assembly that incorporates data from different RNA-seq techniques, with a focus on identifying and...

    Michael A. Schon, Stefan Lutzmayer, ... Michael D. Nodine in Genome Biology
    Article Open access 29 June 2022
  5. Phage DNA Extraction, Genome Assembly, and Genome Closure

    Bacteriophages, or more simply phages, are currently experiencing a renaissance in life science research for their roles in natural microbial...
    Justin Boeckman, Mei Liu, ... Jason Gill in Bacteriophages
    Protocol 2024
  6. Different assembly mechanisms between prokaryotic and fungal communities in grassland plants and soil

    Background and Aims

    Microorganisms play pivotal roles in plant growth and health. However, it remains unclear whether distinct mechanisms govern the...

    Chi Liu, Na Li, ... Minjie Yao in Plant and Soil
    Article 26 April 2024
  7. Regulation of SNARE assembly by protein phosphorylation

    Protein phosphorylation is emerging as an important regulatory mechanism that controls the secretory pathway. It enables coupling between the...
    Adina Weinberger, Jeffrey E. Gerst in Regulatory Mechanisms of Intracellular Membrane Transport
    Chapter
  8. A systematic comparison of human mitochondrial genome assembly tools

    Background

    Mitochondria are the cell organelles that produce most of the chemical energy required to power the cell's biochemical reactions. Despite...

    Nirmal Singh Mahar, Rohit Satyam, ... Ishaan Gupta in BMC Bioinformatics
    Article Open access 13 September 2023
  9. Mass spectrometry uncovers intermediates and off-pathway complexes for SNARE complex assembly

    The SNARE complex assembles from vesicular Synaptobrevin-2 as well as Syntaxin-1 and SNAP25 both anchored to the presynaptic membrane. It mediates...

    Julia Hesselbarth, Carla Schmidt in Communications Biology
    Article Open access 20 February 2023
  10. Elevated methane flux in a tropical peatland post-fire is linked to depth-dependent changes in peat microbiome assembly

    Fires in tropical peatlands extend to depth, transforming them from carbon sinks into methane sources and severely limit forest recovery. Peat...

    Aditya Bandla, Hasan Akhtar, ... Sanjay Swarup in npj Biofilms and Microbiomes
    Article Open access 23 January 2024
  11. Rapid alkalinization factor 22 has a structural and signalling role in root hair cell wall assembly

    Pressurized cells with strong walls make up the hydrostatic skeleton of plants. Assembly and expansion of such stressed walls depend on a family of...

    Sébastjen Schoenaers, Hyun Kyung Lee, ... Kris Vissenberg in Nature Plants
    Article 11 March 2024
  12. De novo assembly of transcriptomes and differential gene expression analysis using short-read data from emerging model organisms – a brief guide

    Many questions in biology benefit greatly from the use of a variety of model systems. High-throughput sequencing methods have been a triumph in the...

    Daniel J. Jackson, Nicolas Cerveau, Nico Posnien in Frontiers in Zoology
    Article Open access 20 June 2024
  13. The Utilization of Reference-Guided Assembly and In Silico Libraries Improves the Draft Genome of Clarias batrachus and Culter alburnus

    Long-read sequencing technologies can generate highly contiguous genome assemblies compared to short-read methods. However, their higher cost often...

    Kai Liu, Nan **e, ... **nyi Liu in Marine Biotechnology
    Article 04 September 2023
  14. Lampshade web spider Ectatosticta davidi chromosome-level genome assembly provides evidence for its phylogenetic position

    The spider of Ectatosticta davidi , belonging to the lamp-shade web spider family, Hypochilidae, which is closely related to Hypochilidae and...

    Zheng Fan, Lu-Yu Wang, ... Ming Bai in Communications Biology
    Article Open access 18 July 2023
  15. Chromosome-level assembly of the Rangifer tarandus genome and validation of cervid and bovid evolution insights

    Background

    Genome assembly into chromosomes facilitates several analyses including cytogenetics, genomics and phylogenetics. Despite rapid development...

    William Poisson, Julien Prunier, ... Claude Robert in BMC Genomics
    Article Open access 23 March 2023
  16. Biocrust and sand burial together promote annual herb community assembly in an arid sandy desert area

    Background

    Annual herbs are crucial components of sandy ecosystems and their community assembly in arid sandy dunes is an intuitive indicator of a...

    Yanhong Gao, Rongliang Jia, ... Wanxue You in Plant and Soil
    Article 07 July 2023
  17. Distinct patterns of distribution, community assembly and cross-domain co-occurrence of planktonic archaea in four major estuaries of China

    Background

    Archaea are key mediators of estuarine biogeochemical cycles, but comprehensive studies comparing archaeal communities among multiple...

    Xuya Hu, Yujie Huang, ... Kai Wang in Environmental Microbiome
    Article Open access 07 October 2023
  18. Ecosystem size-induced environmental fluctuations affect the temporal dynamics of community assembly mechanisms

    Understanding processes that determine community membership and abundance is important for many fields from theoretical community ecology to...

    Raven L. Bier, Máté Vass, ... Silke Langenheder in The ISME Journal
    Article Open access 18 August 2022
  19. TrEMOLO: accurate transposable element allele frequency estimation using long-read sequencing data combining assembly and map**-based approaches

    Transposable Element MOnitoring with LOng-reads (TrEMOLO) is a new software that combines assembly- and map**-based approaches to robustly detect...

    Mourdas Mohamed, François Sabot, ... Séverine Chambeyron in Genome Biology
    Article Open access 03 April 2023
  20. A consensus-based ensemble approach to improve transcriptome assembly

    Background

    Systems-level analyses, such as differential gene expression analysis, co-expression analysis, and metabolic pathway reconstruction, depend...

    Adam Voshall, Sairam Behera, ... Etsuko N. Moriyama in BMC Bioinformatics
    Article Open access 21 October 2021
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