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Reverse engineering environmental metatranscriptomes clarifies best practices for eukaryotic assembly
BackgroundDiverse communities of microbial eukaryotes in the global ocean provide a variety of essential ecosystem services, from primary production...
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MitoHiFi: a python pipeline for mitochondrial genome assembly from PacBio high fidelity reads
BackgroundPacBio high fidelity (HiFi) sequencing reads are both long (15–20 kb) and highly accurate (> Q20). Because of these properties, they have...
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Monomicellar assembly to synthesize structured and functional mesoporous carbonaceous nanomaterials
The large pores of functional mesoporous carbonaceous nanomaterials have broad accessibility, making them efficient substrates for the mass transport...
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Bookend: precise transcript reconstruction with end-guided assembly
We developed Bookend, a package for transcript assembly that incorporates data from different RNA-seq techniques, with a focus on identifying and...
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Phage DNA Extraction, Genome Assembly, and Genome Closure
Bacteriophages, or more simply phages, are currently experiencing a renaissance in life science research for their roles in natural microbial... -
Different assembly mechanisms between prokaryotic and fungal communities in grassland plants and soil
Background and AimsMicroorganisms play pivotal roles in plant growth and health. However, it remains unclear whether distinct mechanisms govern the...
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Regulation of SNARE assembly by protein phosphorylation
Protein phosphorylation is emerging as an important regulatory mechanism that controls the secretory pathway. It enables coupling between the... -
A systematic comparison of human mitochondrial genome assembly tools
BackgroundMitochondria are the cell organelles that produce most of the chemical energy required to power the cell's biochemical reactions. Despite...
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Mass spectrometry uncovers intermediates and off-pathway complexes for SNARE complex assembly
The SNARE complex assembles from vesicular Synaptobrevin-2 as well as Syntaxin-1 and SNAP25 both anchored to the presynaptic membrane. It mediates...
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Elevated methane flux in a tropical peatland post-fire is linked to depth-dependent changes in peat microbiome assembly
Fires in tropical peatlands extend to depth, transforming them from carbon sinks into methane sources and severely limit forest recovery. Peat...
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Rapid alkalinization factor 22 has a structural and signalling role in root hair cell wall assembly
Pressurized cells with strong walls make up the hydrostatic skeleton of plants. Assembly and expansion of such stressed walls depend on a family of...
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De novo assembly of transcriptomes and differential gene expression analysis using short-read data from emerging model organisms – a brief guide
Many questions in biology benefit greatly from the use of a variety of model systems. High-throughput sequencing methods have been a triumph in the...
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The Utilization of Reference-Guided Assembly and In Silico Libraries Improves the Draft Genome of Clarias batrachus and Culter alburnus
Long-read sequencing technologies can generate highly contiguous genome assemblies compared to short-read methods. However, their higher cost often...
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Lampshade web spider Ectatosticta davidi chromosome-level genome assembly provides evidence for its phylogenetic position
The spider of Ectatosticta davidi , belonging to the lamp-shade web spider family, Hypochilidae, which is closely related to Hypochilidae and...
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Chromosome-level assembly of the Rangifer tarandus genome and validation of cervid and bovid evolution insights
BackgroundGenome assembly into chromosomes facilitates several analyses including cytogenetics, genomics and phylogenetics. Despite rapid development...
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Biocrust and sand burial together promote annual herb community assembly in an arid sandy desert area
BackgroundAnnual herbs are crucial components of sandy ecosystems and their community assembly in arid sandy dunes is an intuitive indicator of a...
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Distinct patterns of distribution, community assembly and cross-domain co-occurrence of planktonic archaea in four major estuaries of China
BackgroundArchaea are key mediators of estuarine biogeochemical cycles, but comprehensive studies comparing archaeal communities among multiple...
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Ecosystem size-induced environmental fluctuations affect the temporal dynamics of community assembly mechanisms
Understanding processes that determine community membership and abundance is important for many fields from theoretical community ecology to...
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TrEMOLO: accurate transposable element allele frequency estimation using long-read sequencing data combining assembly and map**-based approaches
Transposable Element MOnitoring with LOng-reads (TrEMOLO) is a new software that combines assembly- and map**-based approaches to robustly detect...
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A consensus-based ensemble approach to improve transcriptome assembly
BackgroundSystems-level analyses, such as differential gene expression analysis, co-expression analysis, and metabolic pathway reconstruction, depend...