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Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins
Light scattering methods permit the determination of molar mass and hydrodynamic radius for a protein from first principles. They are, therefore,...
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Coupling of Membrane Technology with Emerging Technologies for the Recovery of Bioactives
In this chapter an overview of the bioactive extraction techniques by the combination of membrane processes and coupling it with new emerging...
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Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-negative bacteria depends on delivery of unfolded OMPs to the...
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Ultrafiltration and microfiltration membrane performance, cleaning, and flux recovery for microalgal harvesting
Membrane material, pore size, and cleaning strategy are important factors for microalgal membrane harvesting. In this study, harvesting of Scenedesmus...
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Removal Strategies for Aquatic Microplastics
The ubiquitous plastic waste results in a growing concern for environmental pollution about aquatic microplastics (MPs). It calls for efficient...
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Strategies to Isolate Extracellular Vesicles from Gram-Negative and Gram-Positive Bacteria
Membrane vesicles are produced by all Gram-negative and Gram-positive bacteria investigated so far. Membrane vesicles are spherical bilayers of...
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Incorporating support vector machine with sequential minimal optimization to identify anticancer peptides
BackgroundCancer is one of the major causes of death worldwide. To treat cancer, the use of anticancer peptides (ACPs) has attracted increased...
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Purification of Phenolic-Based Molecules from Agro-Food By-products via Pressure-Driven Membrane Processes
Fruit and vegetable processing industries generate huge amounts of highly polluting wastes and by-products because of their high organic load...
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NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix
BackgroundNME6 is a member of the nucleoside diphosphate kinase (NDPK/NME/Nm23) family which has key roles in nucleotide homeostasis, signal...
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Comprehensive isolation of extracellular vesicles and nanoparticles
There is an increasing appreciation for the heterogeneous nature of extracellular vesicles (EVs). In addition, two nonvesicular extracellular...
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Fluorescence Recovery After Photobleaching to Study the Dynamics of Membrane-Bound Proteins In Vivo Using the Drosophila Embryo
The epithelial-to-mesenchymal transition is a highly dynamic cell process and tools such as fluorescence recovery after photobleaching (FRAP), which...
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Large Complexes: Cloning Strategy, Production, and Purification
With few exceptions—such as myxobacteria, filamentous cyanobacteria, and actinomycetes (Rokas, Annu Rev Genet 42:235–251, 2008)—bacteria are defined...
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An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
Green fluorescent proteins (GFPs) are widely used to monitor membrane protein expression, purification, and stability. An ideal reporter should be...
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Muscle Complexes in the Jaw Apparatus of Teleost Fishes: Structure and Function
AbstractThe striated muscles of the teleost fishes sometimes combine into complicated “supramuscular” complexes. In these complexes, individual...
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The Role of Wastewater Treatment Technologies in Municipal Landfill Leachate Treatment
Municipal landfilling is the most common waste management practice to eliminate urban solid waste, but a critical problem coupled with landfills is...
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Purification of Na+-Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic Force Microscopy
The stator unit of the bacterial flagellar motor coordinates the number of active stators in the motor by sensing changes in external load and ion...
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Investigation of Protein–Lipid Interactions Using Native Mass Spectrometry
Integral membrane proteins are embedded in biological membranes where various lipids modulate their structure and function. There exists a critical...
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Industrial Wastewater Treatment in Bio-electrochemical Systems
The age of industrialization has brought a new dawn of global order and has led to advancement of mankind. The dynamics of industrial growth...
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In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion
The GLUT4 vesicle fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and a variety of regulatory...
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Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases
Hedgehog (Hh) signaling culminates in the conversion of the latent transcription factor Cubitus interruptus (Ci)/Gli from a repressor form (CiR/GliR)...