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Establishment of efficient Cannabis (Cannabis sativa L.) protoplast isolation and transient expression condition
There has been a significant increase in interest in Cannabis ( Cannabis sativa L.) plants for the production of secondary metabolites (i.e.,...
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Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis
BackgroundCotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered...
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Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai
BackgroundAngelica Gigas (Purple parsnip) is an important medicinal plant that is cultivated and utilized in Korea, Japan, and China. It contains...
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Development of a general protoplast-mediated regeneration protocol for Brassica: cabbage and cauliflower as examples
The genus Brassica includes vegetables such as broccoli, cabbage, cauliflower, kale, and kohlrabi, which are economically important because they...
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CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration
Clustered regularly interspaced short palindromic repeats (CRISPRCRISPR)-Cas (CRISPR-associated system) has become the multipurpose tool to...
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An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast
BackgroundThe bimolecular fluorescence complementation (BiFC) assay is commonly used for investigating protein–protein interactions. While several...
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A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]
BackgroundTea is the most popular beverage worldwide second only to water. Its demand is tremendously rising due to increased awareness of its...
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Enhanced polyethylene glycol (PEG)–mediated protoplast transformation system for the phytopathogenic fungus, Ganoderma boninense
The basidiomycete fungus, Ganoderma boninense , has been identified as the main causal agent of oil palm basal stem rot (BSR) disease which has caused...
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Protoplast Isolation and Transfection in Rice
Rice (Oryza sativa) is an important cereal crop and a model monocot plant for biology research. The reliable system of foreign DNA transformation and...
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Efficient leaf mesophyll protoplast isolation and transient gene expression in bermudagrasses (Cynodon spp.)
Bermudagrasses ( Cynodon spp. ) are important warm-season turfgrass species with multiple applications. Although physiological and high-throughput...
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Protoplast-mediated transformation in Sporisorium scitamineum facilitates visualization of in planta developmental stages in sugarcane
BackgroundSporisorium scitamineum is the causative agent of smut disease in sugarcane. The tricky life cycle of S. scitamineum consists of three...
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Physcomitrium patens Protoplasting and Protoplast Transfection
Protoplast production with the moss Physcomitrium (Physcomitrella) patens has a long and successful history. As a tool, it has not only been the base...
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Protoplast for Gene Functional Analysis in Arabidopsis
Protoplast, a plant cell without cell wall, can be readily transfected by exogenous macromolecules (DNA, RNA, protein) and therefore offer a...
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Development of PEG-mediated genetic transformation and gene editing system of Bryum argenteum as an abiotic stress tolerance model plant
Key messageTo establish a sterile culture system and protoplast regeneration system for Bryum argenteum , and to establish and apply CRISPR/Cas9...
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Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum)
Key messageWe have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant...
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Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis
Protoplasts are an attractive explant source for biotechnological tools widely used on citrus genetic improvement, such as somatic hybridization and...
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Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis Via Protoplast Transfection
Recent advances in DNA synthesis and assembly allow for genetic constructs to be designed and constructed in high throughput. Characterizing large...
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Advances in protoplast transfection promote efficient CRISPR/Cas9-mediated genome editing in tetraploid potato
Main conclusionAn efficient method of DNA-free gene-editing in potato protoplasts was developed using linearized DNA fragments, UBIQUITIN10 ...
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Determination of protoplast growth properties using quantitative single-cell tracking analysis
BackgroundAlthough quantitative single-cell analysis is frequently applied in animal systems, e.g. to identify novel drugs, similar applications on...
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Protoplast Isolation and Transfection in Wheat
Wheat is one of the major staple crops around the world. A transient expression system is crucial for gene functional studies in wheat as stable...