Search
Search Results
-
Cell Cycle–Specific Protein Phosphatase 1 (PP1) Substrates Identification Using Genetically Modified Cell Lines
The identification of protein phosphatase 1 (PP1) holoenzyme substrates has proven to be a challenging task. PP1 can form different holoenzyme...
-
Live Cell Synthetic Histone Acetylation by Chemical Catalyst
Posttranslational modifications (PTMs) of histonesPosttranslational modifications (PTMs) of histones, such as lysine acetylation and...
-
Purification of Chloroplast Envelope, Thylakoids, and Stroma from Angiosperm Leaves
Plant cell chloroplasts are bounded by a two-membrane envelope. Their photosynthetic function is based on the development of an operational large...
-
Isolation of Glycosomes from Trypanosoma brucei
Glycosomes, belonging to the sub-class of peroxisomes, are single-membrane-bound organelles of trypanosomatid parasites. Glycosomes compartmentalize...
-
Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining
In the adult mouse testis, germ cells of various developmental cell states co-exist. FACS isolation of cells stained with the DNA dye Hoechst 33342...
-
Isosmotic Contraction of Rat Aortic Smooth Muscle Cells upon Activation of Purinergic Receptors: the Role of Chlorine Transport
AbstractWe studied the effect of the purinergic signaling system and the contribution of Cl – transporters to isosmotic contraction of vascular...
-
The trend of allogeneic tendon decellularization: literature review
Tendon injuries repair is a significant burden for orthopaedic surgeons. Finding a proper graft material to repair tendon is one of the main...
-
Zinc Supplementation Reduces Testicular Cell Apoptosis in Mice and Improves Spermatogenic Dysfunction Caused by Marginal Zinc Deficiency
Zinc (Zn) is an important trace element in the human body and plays an important role in growth, development, and male reproductive functions....
-
Extracellular Vesicles: The Challenges on the Way and Engineering Perspectives
Extracellular vesicles (EVs) are found in every body fluid and are known to be released by every human cell. So far, EVs have been classified into...
-
In Vivo and In Vitro Assay to Address Dendritic Cell Antigen Cross-Presenting Capacity
Antigen cross-presentation by dendritic cells is an important pathway to prime CD8+ T cells in infections, cancer, and other immune-mediated...
-
TMEM63B channel is the osmosensor required for thirst drive of interoceptive neurons
Thirst plays a vital role in the regulation of body fluid homeostasis and if deregulated can be life-threatening. Interoceptive neurons in the...
-
DNA Damage Foci on Metaphase Chromosomes
After DNAs are damaged, DNA repair proteinsDNA repair proteins accumulate and are activated at the DNA damaged siteDNA damaged site. These...
-
MS2-MBP-Based Affinity Purification of Nucleus- or Cytoplasm-Localized lncRNA–Protein Complexes Formed In Vivo
With recent emergence of huge number of long noncoding RNAs (lncRNAs), purification of lncRNA–protein (lncRNP) complexes is fundamental to understand...
-
Analyses of Mesenchymal Progenitors in Skeletal Muscle by Fluorescence-Activated Cell Sorting and Tissue Clearing
Mesenchymal progenitors, which are resident progenitor populations residing in skeletal muscle interstitial space, contribute to pathogeneses such as...
-
Characterization and biocompatibility evaluation of acellular rat skin scaffolds for skin tissue engineering applications
Utilization of acellular scaffolds, extracellular matrix (ECM) without cell content, is growing in tissue engineering, due to their high...
-
Platelet-Rich Plasma-Embedded Porous Polycaprolactone Film with a Large Surface Area for Effective Hemostasis
Background:Uncontrollable and widespread bleeding caused by surgery or sudden accidents can lead to death if not treated with appropriate hemostasis....
-
A new storage solution for the hypothermic preservation of corneal grafts: an experimental study
In this experimental study we used for the first time Tiprotec ® as a solution for corneal preservation and cold storage. We compared the resultant...
-
Isolation of Cytosolic Ribosomes Associated with Plant Mitochondria and Chloroplasts
Excluding the few dozen proteins encoded by the chloroplast and mitochondrial genomes, the majority of plant cell proteins are synthesized by...
-
Assessing the Activity of Transcription Factor FoxO1
The transcription factor FoxO1 (forkhead box O1) regulates genes that are involved in development, metabolism, cellular innovation, longevity, and...
-
MpMLO1 controls sperm discharge in liverwort
In bryophytes, sexual reproduction necessitates the release of motile sperm cells from a gametophyte into the environment. Since 1856, this process,...
