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Isolation of the Inner and Outer Membranes of the Chloroplast Envelope from Angiosperms
The outer and the inner membranes of the chloroplast envelope, also called OEM and IEM, have distinct lipid and protein compositions. They host...
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Tissue RNA Integrity in Visium Spatial Protocol (Fresh Frozen Samples)
The transcriptome of a tissue can be acquired both by single-cell RNAseq (scRNA-seq) and by spatial transcriptomics (ST). The dissociation step,...
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Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells
Plant cells can undergo regulated cell death in response to exogenous factors (often in a stress context), but also as regular element of development...
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COX17 acetylation via MOF–KANSL complex promotes mitochondrial integrity and function
Reversible acetylation of mitochondrial proteins is a regulatory mechanism central to adaptive metabolic responses. Yet, how such functionally...
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Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds
Whole-cell biosensors, based on the visualization of a reporter strain’s response to a particular stimulus, are a robust and cost-effective means to...
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Atypical nuclear envelope condensates linked to neurological disorders reveal nucleoporin-directed chaperone activities
DYT1 dystonia is a debilitating neurological movement disorder arising from mutation in the AAA+ ATPase TorsinA. The hallmark of Torsin dysfunction...
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Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis
BackgroundThe increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific...
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Quantity and quality of minichromosome maintenance protein complexes couple replication licensing to genome integrity
Accurate and complete replication of genetic information is a fundamental process of every cell division. The replication licensing is the first...
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H3K9me selectively blocks transcription factor activity and ensures differentiated tissue integrity
The developmental role of histone H3K9 methylation (H3K9me), which typifies heterochromatin, remains unclear. In Caenorhabditis elegans , loss of...
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Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins
The arrangement of centromeres within the nucleus differs among species and cell types. However, neither the mechanisms determining centromere...
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Cell Division in Caulobacter crescentus: A Molecular-Scale Model
During bacterial division, a single mother cell undergoes a stereotyped shape change to split into two daughter cells. The division process is...
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The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis
The Tol–Pal system of Gram-negative bacteria is necessary for maintaining outer membrane integrity. It is a multiprotein complex of five envelope...
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Plastid Transient and Stable Interactions with Other Cell Compartments
Plastids are organelles delineated by two envelopes playing important roles in different cellular processes such as energy production or lipid...
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Strong interactions between highly dynamic lamina-associated domains and the nuclear envelope stabilize the 3D architecture of Drosophila interphase chromatin
BackgroundInteractions among topologically associating domains (TADs), and between the nuclear envelope (NE) and lamina-associated domains (LADs) are...
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Deformation of the nucleus by TGFβ1 via the remodeling of nuclear envelope and histone isoforms
The cause of nuclear shape abnormalities which are often seen in pre-neoplastic and malignant tissues is not clear. In this study we report that...
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Translocation of non-lytic antimicrobial peptides and bacteria penetrating peptides across the inner membrane of the bacterial envelope
The increase in multidrug-resistant pathogenic bacteria has become a problem worldwide. Currently there is a strong focus on the development of novel...
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Cell Migration in Three Dimensions
Cell migration plays an essential role in many pathophysiological processes, including embryonic development, wound healing, immunity, and cancer...
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Environmentally relevant fluoride alters nuclear integrity in erythrocytes and induces DNA damage in hepatocytes of zebrafish
The toxicity associated with environmentally relevant concentration of fluoride (F) was investigated in zebrafish ( Danio rerio ). Following exposure...
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FacZ is a GpsB-interacting protein that prevents aberrant division-site placement in Staphylococcus aureus
Staphylococcus aureus is a Gram-positive pathogen responsible for antibiotic-resistant infections. To identify vulnerabilities in cell envelope...
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Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation
Neutrophils migrating towards chemoattractant gradients amplify their recruitment range by releasing the secondary chemoattractant leukotriene B 4 (LTB
