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Phytoremediation: a transgenic perspective in omics era
Phytoremediation is an environmental safety strategy that might serve as a viable preventative approach to reduce soil contamination in a...
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Stress responsive ZmWRKY53 gene increases cold tolerance in rice
Plant WRKY transcription factors are responsible for biotic and abiotic stresses and play an important role in enhancing their adaptability. The AtWRKY33...
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Plant characterization of insect-protected soybean
Insect-protected soybean (SIP) that produces the Cry1A.105 and Cry2Ab2 insecticidal crystal proteins has been developed to provide protection from...
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Expression of a single-chain monellin (MNEI) mutant with enhanced stability in transgenic mice milk
Monellin is a sweet protein that may be used as a safe and healthy sweetener. However, due to its low stability, the application of monellin is...
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The role of microRNAs in NBS-LRR gene expression and its implications for plant immunity and crop development
Plants evolved, over millions of years, complex defense systems against pathogens. Once infected, the interaction between pathogen effector molecules...
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Developmental and therapeutic implications of IL4ra expression for rhabdomyosarcoma
Rhabdomyosarcoma (RMS) is a solid tumor whose metastatic progression can be accelerated through interleukin-4 receptor alpha (Il4ra) mediated...
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Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant
RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artificial microRNA (amiRNA) can be engineered to...
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Introgression of Δ1-pyrroline-5-carboxylate synthetase (PgP5CS) confers enhanced resistance to abiotic stresses in transgenic tobacco
Δ 1 -pyrroline-5-carboxylate synthetase ( P5CS ) is one of the key regulatory enzymes involved in the proline biosynthetic pathway. Proline acts as an...
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Coexistence field trials between MON810 and conventional maize in Mallorca as a basis for a regional regulatory proposal based on scientific evidence in the times of genome editing
This paper reports the first coexistence field trials between transgenic and conventional maize carried out under Mediterranean island conditions....
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Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP
Golli–myelin basic proteins, encoded by the myelin basic protein gene, are widely expressed in neurons and oligodendrocytes in the central nervous...
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Regulatory landscape and public perception for gene-edited bananas in the Southeast Asian region
Banana is a premier fruit crop in many parts of the world especially Southeast Asia. The demand for banana has contributed to significant national...
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Prey-mediated effects of Mpp51Aa2-producing cotton on longevity and reproduction of Orius majusculus
Genetically engineered (GE) cotton event MON 88702, producing Mpp51Aa2 (previously mCry51Aa2) from Bacillus thuringiensis (Bt), controls sucking...
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Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco
Plants can produce complex pharmaceutical and technical proteins. Spider silk proteins are one example of the latter and can be used, for example, as...
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The SpRY Cas9 variant release the PAM sequence constraint for genome editing in the model plant Physcomitrium patens
Genome editing via CRISPR/Cas has enabled targeted genetic modifications in various species, including plants. The requirement for specific...
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Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene
Several expression systems have been developed in clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9...
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Establishment of a hydrodynamic delivery system in ducks
Chronic hepatitis B virus (HBV) poses a significant global health challenge as it can lead to acute or chronic liver disease and hepatocellular...
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Specific activity and utility of recombinant cellobiohydrolase II (Cel6A) produced in maize endosperm
Cellobiohydrolase II (CBH II) is an exo-glucanase that is part of a fungal mixture of enzymes from a wood-rot fungus, Trichoderma reesei . It is...
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Efficiency of the alpha-hairpinin SmAMP-X gene promoter from Stellaria media plant depends on selection of transgenic approach
The antimicrobial activity of the alpha-HAIRPININ ANTIMICROBIAL PEPTIDE X ( SmAMP-X gene, GenBank acc. No. HG423454.1) from Stellaria media plant has...
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Usefulness of current sgRNA design guidelines and in vitro cleavage assays for plant CRISPR/Cas genome editing: a case targeting the polyphenol oxidase gene family in eggplant (Solanum melongena L.)
The advent of genome editing platforms such as the CRISPR/Cas9 system ushers an unprecedented speed in the development of new crop varieties that can...