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Comparison of ordinary reverse transcription real-time polymerase chain reaction (qRT-PCR) with a newly developed one-step single-tube nested real-time RT-PCR (OSN-qRT-PCR) for sensitive detection of SARS-CoV-2 in wastewater
Wastewater monitoring has proven to be an important approach to detecting and controlling the development of the SARS-CoV-2 pandemic. Various tests...
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A new TaqMan real-time PCR assay to detect Parachlamydia acanthamoebae and to monitor its co-existence with SARS-COV-2 among COVID-19 patients
Human respiratory infections caused by a large variety of microbial pathogens are the most common diseases responsible for hospitalization, morbidity...
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Real-time detection of ammonium in soil pore water
The development of technologies for continuous measurement of nitrogen forms in the soil is essential for optimizing the application of fertilizers...
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Reliability of Covid-19 PCR Test Results with Statistical Distributions
COVID-19, the causative agent being severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been one of the most contagious diseases in the...
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Rapid monitoring of indoor airborne influenza and coronavirus with high air flowrate electrostatic sampling and PCR analysis
The World Health Organization has raised concerns about the possibility of airborne transmission in enclosed and poorly ventilated areas. Therefore,...
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Genes regulating development and behavior exhibited altered expression in Drosophila melanogaster exposed to bisphenol A: use of real-time quantitative PCR (qRT-PCR) and droplet digital PCR (ddPCR) in genotoxicity study
Toxicity of bisphenol A on morphological and life-history traits of model insect Drosophila melanogaster was reported in our previous work. In the...
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Microarray analysis of mRNA expression profiles in liver of ob/ob mice with real-time atmospheric PM2.5 exposure
Epidemiological studies have demonstrated the association between exposure to fine particulate matter (PM 2.5 ) and the onset of non-alcoholic fatty...
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Development of Rapid PCR Methods for the Detection and Quantification of Geosmin-Producing Dolichospermum spp.
Geosmin, produced by cyanobacteria and actinomycetes, is a common cause of earthy odor in lakes, rivers, and reservoirs. Drinking water quality...
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Quantitative RT-PCR detection of human noroviruses and hepatitis A virus in fresh produce and surface water used for irrigation in the Mansoura and Giza regions, Egypt
Surface water used as an irrigation source can be a significant source of viral contamination of fresh produce. Enteric viruses such as hepatitis A...
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q-PCR-based assay for the toxic dinoflagellate Karenia selliformis monitoring along the Tunisian coasts
Karenia selliformis is a marine dinoflagellate responsible for fish-kill events. Its presence has been reported along the Tunisian coasts...
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Selection of reference genes for normalizing gene expression data across seasons in spermatozoa of water buffalo (Bubalus bubalis)
Selection of the most stably expressed reference genes is key to monitoring accurate target gene expression across any tissue or cell type. The mRNA...
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Monitoring of enteropathogenic Gram-negative bacteria in wastewater treatment plants: a multimethod approach
The wastewater treatment processes are associated with the emission of microbial aerosols, including enteropathogenic bacteria. Their presence in...
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Assessing soil extracellular DNA decomposition dynamics through plasmid amendment coupled with real-time PCR
PurposeDetermining soil extracellular DNA decomposition dynamics is essential to assessing lateral gene transfer possibility, nutrient-cycling...
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Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source
Polyhydroxyalkanoates have attracted great interest as a suitable alternative to petrochemical based plastics due to their outstanding properties...
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Effects of chloride, sulfate, and bicarbonate stress on mortality rate, gill tissue morphology, and gene expression in mandarin fish (Siniperca chuatsi)
The mandarin fish Siniperca chuatsi is a freshwater fish that is endemic to East Asia. To study the different damages and molecular mechanisms caused...
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Effect of hexavalent chromium exposure on the reproductive status and biomarker responses of female Geloina erosa
Hexavalent chromium (Cr (VI)) is widely distributed in the marine environment of Hainan Province, China and poses a potential threat to its mangrove...
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Preliminary evidence on the presence of cyanobacteria and cyanotoxins from culture enrichments followed by PCR analysis: new perspectives from Africa (Mali) and South Pacific (Fiji) countries
Cyanobacteria are a group of microorganisms that can be found in a diverse range of biogeographical areas and produce potent and damaging...
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Comparison of the bacterial viability assessments for the disinfected quarantined water along with an effect of total residual oxidants
The water discarded from the quarantine station inspecting aquatic products can be served as an influx channel of invasive microorganisms to our own...
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Curcumin strengthens a spontaneous self-protective mechanism-SP1/PRDX6 pathway, against di-n-butyl phthalate-induced testicular ferroptosis damage
As one of the common plasticizers, di-n-butyl phthalate (DBP) has been using in various daily consumer products worldwide. Since it is easily...
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Temporal dynamics and persistence of resistance genes to broad spectrum antibiotics in an urban community
The constantly evolving and growing global health crisis, antimicrobial resistance (AMR), endangers progress in medicine, food production, and life...
