Search
Search Results
-
Non-specificity as the sticky problem in therapeutic antibody development
Antibodies are highly potent therapeutic scaffolds with more than a hundred different products approved on the market. Successful development of...
-
Functional spectrum and specificity of mitochondrial ferredoxins FDX1 and FDX2
Ferredoxins comprise a large family of iron–sulfur (Fe–S) proteins that shuttle electrons in diverse biological processes. Human mitochondria contain...
-
Assaying for Arginyltransferase Activity and Specificity by Peptide Arrays
Here, we describe arginylation assays performed on peptide arrays immobilized on cellulose membranes via chemical synthesis. In this assay, it is...
-
Integrating automated liquid handling in the separation workflow of extracellular vesicles enhances specificity and reproducibility
BackgroundExtracellular vesicles (EV) are extensively studied in human body fluids as potential biomarkers for numerous diseases. Major impediments...
-
Molecular basis and engineering of miniature Cas12f with C-rich PAM specificity
CRISPR–Cas12f nucleases are currently one of the smallest genome editors, exhibiting advantages for efficient delivery via cargo-size-limited...
-
Specificity of Interionic Interaction As a Factor in the Selective Extraction of “Soft” Anions from Aqueous Media Using 2(4)-Octylaminopyridines
AbstractThe specificity of interionic interaction in associates with inorganic anions is discussed, based on results from physical studies of...
-
Substrate specificity map** of fungal CAZy AA3_2 oxidoreductases
BackgroundOxidative enzymes targeting lignocellulosic substrates are presently classified into various auxiliary activity (AA) families within the...
-
The Global Acetylation Profiling Pipeline for Quick Assessment of Protein N-Acetyltransferase Specificity In Cellulo
Global acetylation profiling (GAP) consists of heterologous expression of a given N-acetyltransferase (NAT) in Escherichia coli to assess its...
-
Effect of Magnetite Content and Specificity of Nickel(II) Ions on Electrokinetic Properties of Composites Based on Porous Silica Particles
AbstractPowders of magnetite and its composites have been obtained on the basis of macroporous high-silica glass particles containing different...
-
Enhancing the Authentication Capability of Triplex Real-Time PCR by Increasing the Primer Specificity
Authenticity identification is crucial for the legal control of meat fraud and adulteration. Authentication capability of a method in mixed meat is...
-
Enhancing 2-O-α-D-glucopyranosyl-l-ascorbic acid synthesis by weakening the acceptor specificity of CGTase toward glucose and maltose
2-O-α-D-glucopyranosyl- l -ascorbic acid (AA-2G) is a stable derivative of l -ascorbic acid ( l -AA), which has been widely used in food and cosmetics...
-
A mitochondria-targeted AIE photosensitizer for enhancing specificity and efficacy of ferroptosis inducer
Ferroptosis is a form of regulated cell death characterized by iron-dependent accumulation of lipid peroxidation and lethal reactive oxygen species...
-
Heterotrimeric collagen helix with high specificity of assembly results in a rapid rate of folding
The most abundant natural collagens form heterotrimeric triple helices. Synthetic mimics of collagen heterotrimers have been found to fold slowly,...
-
Enhanced Substrate Specificity of Thermostable Cytochrome P450 CYP175A1 by Site Saturation Mutation on Tyrosine 68
Thermostable cytochrome P450 (CYP175A1) cloned from Thermus thermophilus shows mid-point unfolding temperature (Tm) of 88 °C (361 K) along with high...
-
The Role of a Loop in the Non-catalytic Domain B on the Hydrolysis/Transglycosylation Specificity of the 4-α-Glucanotransferase from Thermotoga maritima
The mechanism by which glycoside hydrolases control the reaction specificity through hydrolysis or transglycosylation is a key element embedded in...
-
Designer installation of a substrate recruitment domain to tailor enzyme specificity
Promiscuous enzymes that modify peptides and proteins are powerful tools for labeling biomolecules; however, directing these modifications to desired...
-
Study of the Mechanism of Coenzyme Specificity of Phenylacetone Monooxygenase from Thermobifida fusca by Site-Directed Mutagenesis
AbstractPhenylacetone monooxygenase from Thermobifida fusca (EC 1.14.13.92, PAMO) belongs to the Baeyer–Villiger family of monooxygenases and...
-
Regulation of acetate tolerance by small ORF-encoded polypeptides modulating efflux pump specificity in Methylomonas sp. DH-1
BackgroundMethanotrophs have emerged as promising hosts for the biological conversion of methane into value-added chemicals, including various...
-
An orthogonalized PYR1-based CID module with reprogrammable ligand-binding specificity
Plants sense abscisic acid (ABA) using chemical-induced dimerization (CID) modules, including the receptor PYR1 and HAB1, a phosphatase inhibited by...
-
Silver-Doped Nano-DESI MSI for Increased Specificity and Sensitivity of Alkenes
Nanospray desorption electrospray ionization (nano-DESI) is a technique that can be used for mass spectrometry imaging (MSI) experiments. Due to the...