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Construction of a tunable promoter library to optimize gene expression in Methylomonas sp. DH-1, a methanotroph, and its application to cadaverine production
BackgroundAs methane is 84 times more potent than carbon dioxide in exacerbating the greenhouse effect, there is an increasing interest in the...
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Genome Scale Metabolic Model of the versatile methanotroph Methylocella silvestris
BackgroundMethylocella silvestris is a facultative aerobic methanotrophic bacterium which uses not only methane, but also other alkanes such as...
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Boosting the acetol production in methanotrophic biocatalyst Methylomonas sp. DH-1 by the coupling activity of heteroexpressed novel protein PmoD with endogenous particulate methane monooxygenase
BackgroundMethylacidiphilum sp. IT6 has been validated its C3 substrate assimilation pathway via acetol as a key intermediate using the PmoCAB3, a...
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Reconstruction of a Genome Scale Metabolic Model of the polyhydroxybutyrate producing methanotroph Methylocystis parvus OBBP
BackgroundMethylocystis parvus is a type II methanotroph characterized by its high specific methane degradation rate (compared to other...
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An Overview on Methanotrophs and the Role of Methylosinus trichosporium OB3b for Biotechnological Applications
Rising greenhouse gas concentrations in the atmosphere are having deleterious effects on biotic and abiotic systems, causing serious global concerns....
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Methylosinus trichosporium OB3b bioaugmentation unleashes polyhydroxybutyrate-accumulating potential in waste-activated sludge
BackgroundWastewater treatment plants contribute approximately 6% of anthropogenic methane emissions. Methanotrophs, capable of converting methane...
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Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds
Methane is considered the carbon source with the highest potential in industrial biotechnology because of its abundance and sustainability. The...
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A novel inverse membrane bioreactor for efficient bioconversion from methane gas to liquid methanol using a microbial gas-phase reaction
BackgroundMethane (CH 4 ), as one of the major energy sources, easily escapes from the supply chain into the atmosphere, because it exists in a...
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Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z
BackgroundEctoine (1,3,4,5-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) is an attractive compatible solute because of its wide industrial...
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Methane Based Continuous Culture of Methylosinus trichosporium for Production of Poly-3-hydroxybutyrate Using Membrane Recycle System
Poly-3-hydroxybutyrate (PHB) is a bio-based and biodegradable polymer produced by microbial fermentation from wide variety of feedstocks....
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Hydrogen Production from Methane by Methylomonas sp. DH-1 under Micro-aerobic Conditions
Fueled by the recognition of hydrogen as a promising renewable energy source for the future, there have been many attempts to find greener and more...
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A dynamic model of growth phase of bio-conversion of methane to polyhydroxybutyrate using dynamic flux balance analysis
Biological conversion of waste methane to biodegradable plastics is a way of reducing their production cost. This study addresses the computational...
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Product analogue binding identifies the copper active site of particulate methane monooxygenase
Nature’s primary methane-oxidizing enzyme, the membrane-bound particulate methane monooxygenase (pMMO), catalyses the oxidation of methane to...
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Microfluidics-driven high-throughput phenoty** and screening in synthetic biology: from single cells to cell-free systems
The interdisciplinary nature of synthetic biology merges engineering principles with biology and provides innovative solutions for issues in the...
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Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution
BackgroundMethane, a main component of natural gas and biogas, has gained much attention as an abundant and low-cost carbon source. Methanotrophs,...
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Study on the mechanism of action of methane production by co-fermentation of sludge and lignite
To improve the methanogenic efficiency of lignite anaerobic fermentation and explore innovative approaches to sludge utilization, a co-fermentation...
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Methanol bioconversion in Methylotuvimicrobium buryatense 5GB1C through self-cycling fermentation
Methanol is an abundant and low-cost next-generation carbon source. While many species of methanotrophic bacteria can convert methanol into valuable...
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Turning C1-gases to isobutanol towards great environmental and economic sustainability via innovative biological routes: two birds with one stone
BackgroundThe dramatic increase in greenhouse gas (GHG) emissions, which causes serious global environmental issues and severe climate changes, has...
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Comparative 16S Metabarcoding of Nile Tilapia Gut Microbiota from the Northern Lakes of Egypt
Nile tilapia, Oreochromis niloticus , is the principal fish bred in Egypt. A pilot study was designed to analyze the bacterial composition of the Nile...
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Implications of Polyextremophiles in Astrobiology Research
Astrobiology seeks to expand our knowledge of life by investigating how microorganisms survive and thrive in extreme environments, allowing us to...