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Development of CD163 receptor-based enzyme-linked immunosorbent assay for diagnosis of porcine reproductive and respiratory syndrome virus
Porcine reproductive and respiratory syndrome (PRRS) is an important economical disease in the global swine industry. The accurate detection of the...
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Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer
BackgroundNever in mitosis gene-A (NIMA)-related expressed kinase 2 (NEK2) is a serine/threonine protein kinase regulated by the cell cycle. The...
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BCMA/CD47-directed universal CAR-T cells exhibit excellent antitumor activity in multiple myeloma
BackgroundBCMA-directed autologous chimeric antigen receptor T (CAR-T) cells have shown excellent clinical efficacy in relapsed or refractory...
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In Silico Analysis and Immune Response of YaeT Protein Against Riemerella anatipestifer in Ducks
Riemerella anatipestifer ( RA ) is one of the most harmful bacterial pathogens in waterfowl and causes enormous economic loss worldwide. Due to weak...
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Novel enzyme-linked aptamer-antibody sandwich assay and hybrid lateral flow strip for SARS-CoV-2 detection
We have successfully generated oligonucleotide aptamers (Apts) and monoclonal antibodies (mAbs) targeting the recombinant nucleocapsid (N) protein of...
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Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken
BackgroundSalmonella Enteritidis ( S. Enteritidis ) being one of the most prevalent foodborne pathogens worldwide poses a serious threat to public...
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Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics
BackgroundLeukocyte phytohemagglutinin (PHA-L), derived from the L4 tetramer of PHA, has been frequently employed as a mitogen to induce T lymphocyte...
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Production of monoclonal antibody against tylosin and tilmicosin with homogeneous cross-reactivity and its application in lateral flow immunoassay
To avoid false negative results due to the low cross-reactivity rate (CR) in rapid immunoassay, a group-specific antibody with homogeneous CR toward...
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Development of a magnetic separation immunoassay with high sensitivity and time-saving for detecting aflatoxin B1 in agricultural crops using nanobody
Detection methods with high sensitivity and short assay time are urgently required for quantitative analysis of small-molecule hazardous substances...
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A nanobody‐horseradish peroxidase fusion protein‐based competitive ELISA for rapid detection of antibodies against porcine circovirus type 2
BackgroundThe widespread popularity of porcine circovirus type 2(PCV2) has seriously affected the healthy development of the pig industry and caused...
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Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen
BackgroundBrucellosis remains one of the global health concerns that reemerges in recent years. Delayed or inaccurate diagnosis end to a long...
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Screening of monoclonal antibodies against specific phosphonylation sites and analysis of serum samples exposed to soman and VX using an indirect competitive enzyme-linked immunosorbent assay
Organophosphorus nerve agents (OPNAs) covalently bind to tyrosine 411 of human serum albumin (HSA) and the formed adducts are stable biomarkers of...
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Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis
BackgroundHuman chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the...
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Preparation of a novel monoclonal antibody against caprine interleukin-17A and its applications in immunofluorescence and immunohistochemistry assays
BackgroundInterleukin-17 (IL-17), the characteristic cytokine secreted by T helper 17 lymphocytes (Th17 cells), plays a pivotal role in host defense...
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Expression of deleted, atoxic atypical recombinant beta2 toxin in a baculovirus system and production of polyclonal and monoclonal antibodies
BackgroundClostridium perfringens is an important animal and human pathogen that can produce more than 16 different major and minor toxins. The...
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Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick
Artesunate is a frontline antimalarial drug for treating Plasmodium falciparum malaria. To produce specific antibodies to artesunate, the carboxyl...
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Development of an Indirect Competitive ELISA Kit for the Detection of Soybean Allergenic Protein Gly m Bd 28K
The complementary DNA (cDNA) sequence of Gly m Bd 28K was expressed in Escherichia coli BL21 (DE3) as an inclusion body under the induction of...
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Influence of Different Processing Treatments on the Detectability of Nucleic Acid and Protein Targets in Transgenic Soybean Meal
Influences of dry heating, wet heating, and extrusion on the degradation of DNA and protein from transgenic soybean meal (TSM) were analyzed using...
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Preparation of Artificial Antigen and Development of Indirect Competitive ELISA Based on Chicken IgY for the Detection of Acid Orange II in Food Samples
To evaluate the feasibility of specific egg yolk antibodies (IgY) technology for the detection of hazardous chemical residues in foodstuffs, IgY were...
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An immunoassay based on bio-barcode method for quantitative detection of semicarbazide
A functionalized gold nanoparticles bio-barcode technology was developed to detect semicarbazide. In the assay, the hapten CPSEM...
