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Fundamentals and Industrial Applications of Modern Genetic Engineering
Biotechnological innovations have become crucial for the growth of three major industrial sectors of economy: fuel, pharmaceuticals and food. Genetic...
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Paul Berg (1926–2023)—“father of genetic engineering,” organizer of the Asilomar meeting
The Nobel laureate Paul Berg initiated genetic manipulation and pioneered genetic engineering. He was also one of the initiators of setting up...
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Screening and genetic engineering of marine-derived Aspergillus terreus for high-efficient production of lovastatin
BackgroundLovastatin has widespread applications thanks to its multiple pharmacological effects. Fermentation by filamentous fungi represents the...
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Genetic Engineering of Trichoderma reesei for Biomass Hydrolysis
The filamentous fungus Trichoderma reesei, a potent cellulase producer, is the most-studied cellulolytic fungus. This fungus is an efficient cell...
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Development of a genetic engineering toolbox for syngas-utilizing acetogen Clostridium sp. AWRP
BackgroundClostridium sp. AWRP (AWRP) is a novel acetogenic bacterium isolated under high partial pressure of carbon monoxide (CO) and can be one of...
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Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum
BackgroundRaw starch-degrading enzyme (RSDE) is applied in biorefining of starch to produce biofuels efficiently and economically. At present, RSDE...
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Genetic improvement in edible fish: status, constraints, and prospects on CRISPR-based genome engineering
Conventional selective breeding in aquaculture has been effective in genetically enhancing economic traits like growth and disease resistance....
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Enhancement of acarbose production by genetic engineering and fed-batch fermentation strategy in Actinoplanes sp. SIPI12-34
BackgroundAcarbose, as an alpha-glucosidase inhibitor, is widely used clinically to treat type II diabetes. In its industrial production, Actinoplanes ...
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Yield Enhancement in Algal Production and Genetic Engineering Tools for Microalgal Biorefinery
Addressing the ecological constraints that the globe is currently facing necessitates the development of food, feed, and bioproducts that are...
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Metabolic Engineering
Metabolic engineering is a powerful methodology aimed at intelligently designing new biological pathways, systems, and ultimately phenotypes...
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Improvement of Soybean; A Way Forward Transition from Genetic Engineering to New Plant Breeding Technologies
Soybean is considered one of the important crops among legumes. Due to high nutritional contents in seed (proteins, sugars, oil, fatty acids, and...
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Rice and Arabidopsis BBX proteins: toward genetic engineering of abiotic stress resistant crops
Productivity of crop plants are enormously affected by biotic and abiotic stresses. The co-occurrence of several abiotic stresses may lead to death...
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Develo** a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways
BackgroundDevelo** new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of...
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Recent advancements in flavonoid production through engineering microbial systems
Flavonoids are a class of polyphenolic compounds found in plants that offer extensive health benefits and have applications in the pharmaceutical,...
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Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria
Current industrial bioethanol production by yeast through fermentation generates carbon dioxide. Carbon neutral bioethanol production by...
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Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering
BackgroundThe Lactobacillaceae family comprises many species of great importance for the food and healthcare industries, with numerous strains...
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Genetic Engineering Strategies and Degradation of Pollutants Using Genetically Engineered Microorganisms (GEMs)
To keep the environment clean and safe, degradation of pollutants is a serious concern in the current scenario. Use of microorganisms in this regard...
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Improvement in l-ornithine production from mannitol via transcriptome-guided genetic engineering in Corynebacterium glutamicum
Backgroundl -Ornithine is an important medicinal intermediate that is mainly produced by microbial fermentation using glucose as the substrate. To...
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100 Years of insulin: A chemical engineering perspective
Insulin was isolated in 1921, with the first human patient treated in 1922, and large-scale production began in 1923. In this perspectives article I...