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Evaluation of Methylotrophic Yeast Ogataea thermomethanolica TBRC 656 as a Heterologous Host for Production of an Animal Vaccine Candidate
Multiple yeast strains have been developed into versatile heterologous protein expression platforms. Earlier works showed that Ogataea...
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Genome-scale model reconstruction of the methylotrophic yeast Ogataea polymorpha
BackgroundOgataea polymorpha is a thermotolerant, methylotrophic yeast with significant industrial applications. While previously mainly used for...
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Yeast Cell Factory for Production of Biomolecules
In the 1860s, Louis Pasteur first demonstrated the relationship between wine fermentation and yeast, which was later studied by different scientists...
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Methanol bioconversion into C3, C4, and C5 platform chemicals by the yeast Ogataea polymorpha
BackgroundOne carbon (C1) molecules such as methanol have the potential to become sustainable feedstocks for biotechnological processes, as they can...
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Unlocking the potential of feruloyl esterase from Myceliophthora verrucosa: a key player in efficient conversion of biorefinery-relevant pretreated rice straw
The lignocellulolytic accessory enzyme, Feruloyl esterase C (FE_5DR), encoded in the genome of thermotolerant Myceliophthora verrucosa was...
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Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast, Ogataea (Hansenula) polymorpha
BackgroundOgataea (Hansenula) polymorpha is one of the most thermotolerant xylose-fermenting yeast species reported to date. Several metabolic...
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High-Level Expression and Biochemical Characterization of a Maltotetraose Amylase in Pichia pastoris X-33 for Maltotetraose Production
Maltotetraose amylase, which catalyzes the hydrolysis of amylaceous polysaccharides into maltooligosaccharides with maltotetraose as the main...
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Yeast Expression Systems: Overview and Recent Advances
Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been...
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Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast
BackgroundXylose transport is one of the bottlenecks in the conversion of lignocellulosic biomass to ethanol. Xylose consumption by the wild-type...
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Established and Upcoming Yeast Expression Systems
Yeast was the first microorganism used by mankind for biotransformation of feedstock that laid the foundations of industrial biotechnology. Long...
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Bioethanolic yeasts from dung beetles: tap** the potential of extremophilic yeasts for improvement of lignocellulolytic feedstock fermentation
Bioethanol from abundant and inexpensive agricultural and industrial wastes possesses the potential to reduce greenhouse gas emissions. Bioethanol as...
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Aerobic Utilization of Methanol for Microbial Growth and Production
Methanol is a reduced one-carbon (C1) compound. It supports growth of aerobic methylotrophs that gain ATP from reduced redox equivalents by...
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Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha
The thermotolerant methylotrophic yeast Hansenula polymorpha has been used as a host for the high-level production of recombinant proteins from...
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Diversity of Thermophilic Prokaryotes
The term ”thermophilic prokaryotes” covers an immense taxonomic and functional diversity of bacteria and archaea, spanning the length and breadth of...
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Recent insights, applications and prospects of xylose reductase: a futuristic enzyme for xylitol production
Xylose reductase (XR) is an intermediate inducible enzyme of xylose metabolism responsible for the reduction of xylose into xylitol. It is an...
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Comparison of Thermal Stability of New Formate Dehydrogenases by Differential Scanning Calorimetry
Formate dehydrogenases (FDHs) from different sources are systematically studied in our laboratory. Over the past few years, new genes of four FDHs...
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Characterization of a novel thermotolerant NAD+-dependent formate dehydrogenase from hot climate plant cotton (Gossypium hirsutum L.)
NAD + -dependent formate dehydrogenases (FDH, EC 1.2.1.2), providing energy to the cell in methylotrophic microorganisms, are stress proteins in higher...
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Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
BackgroundThe thermotolerant methylotrophic yeast Ogataea polymorpha has been regarded as an important organism for basic research and...
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CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications
Non-conventional yeasts are playing important roles as cell factories for bioproduction of biofuels, food additives and proteins with outstanding...
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Transcriptional activator Cat8 is involved in regulation of xylose alcoholic fermentation in the thermotolerant yeast Ogataea (Hansenula) polymorpha
BackgroundEfficient xylose alcoholic fermentation is one of the key to a successful lignocellulosic ethanol production. However, regulation of this...
