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A stepwise mutagenesis approach using histidine and acidic amino acid to engineer highly pH-dependent protein switches
Antibody-based drugs can be highly toxic, because they target normal tissue as well as tumor tissue. The pH value of the extracellular...
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Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
BackgroundClassical directed evolution is a powerful approach for engineering biomolecules with improved or novel functions. However, it...
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Consensus holistic virtual screening for drug discovery: a novel machine learning model approach
In drug discovery, virtual screening is crucial for identifying potential hit compounds. This study aims to present a novel pipeline that employs...
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Functional Peptides from One-bead One-compound High-throughput Screening Technique
C ombinatorial chemistry provides a cost-effective method for the rapid discovery of new functional peptides. One-bead one-compound(OBOC)...
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Multi-layered Microfluidic Drug Screening Platform Enabling Simultaneous Generation of Linear and Logarithmic Concentration Gradients
Since many microfluidic devices have limited drug dose order of gradients and incorporate 2D cell culture, we here present a multi-layered platform...
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Argonaute with stepwise endonuclease activity promotes specific and multiplex nucleic acid detection
Argonaute proteins (Agos) from thermophiles function as endonucleases via guide-target base-pairing cleavage for host defense. Since guides play a...
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Machine-learning screening of luminogens with aggregation-induced emission characteristics for fluorescence imaging
Due to the excellent biocompatible physicochemical performance, luminogens with aggregation-induced emission (AIEgens) characteristics have played a...
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Clinical Applications of Mass Spectrometry in Drug Analysis Methods and Protocols
This fully updated volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Exploring the versatility and...
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Functional metagenomic screening identifies an unexpected β-glucuronidase
The abundance of recorded protein sequence data stands in contrast to the small number of experimentally verified functional annotation. Here we...
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A proposed stepwise screening framework for the selection of polycyclic aromatic hydrocarbon (PAH)-degrading white rot fungi
This study suggests a simple three-step screening protocol for the selection of white rot fungi (WRF) capable of degrading polycyclic aromatic...
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Multidisciplinary Methods for Screening Toxic Proteins from Phages and Their Potential Molecular Targets
This chapter presents a comprehensive methodology for the identification, characterization, and functional analyses of potentially toxic hypothetical...
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A combined ligand and target-based virtual screening strategy to repurpose drugs as putrescine uptake inhibitors with trypanocidal activity
Chagas disease, also known as American trypanosomiasis, is a neglected tropical disease caused by the protozoa Trypanosoma cruzi , affecting nearly...
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Development of a chemogenomics library for phenotypic screening
With the development of advanced technologies in cell-based phenotypic screening, phenotypic drug discovery (PDD) strategies have re-emerged as...
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Construction of a Membrane Yeast Two-Hybrid Library and Screening of MsPYR1-Like Interacting Proteins in Malus sieversii
To investigate the biological effects of the ABA receptor pyrabactin resistance 1-like ( PYR1-like ) in Malus sieversii seeds, the proteins interacting...
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Screening Strategies for Biosurfactant Discovery
The isolation and screening of bacteria and fungi for the production of surface-active compounds has been the basis for the majority of the...
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Comprehensive Urine Drug Screen by Gas Chromatography-Mass Spectrometry (GC-MS)
Drug screening is an important diagnostic tool in patient management and is an indispensable part of clinical toxicology laboratory services. While...
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Screening of Organic Acidurias by Gas Chromatography–Mass Spectrometry (GC–MS)
Organic acidurias or acidemias are a group of diverse disorders caused by decreased or diminished activity of specific enzyme or transporter involved...
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Antiplasmodial potential of phytochemicals from Citrus aurantifolia peels: a comprehensive in vitro and in silico study
Phytochemical investigation of Key lime ( Citrus aurantifolia L., F. Rutaceae) peels afforded six metabolites, known as methyl isolimonate acetate...
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Drug Screening of 49 Compounds by Liquid Chromatography-Tandem Mass Spectrometry in Saliva
In recent years, the use of oral fluid as a testing matrix for drug analysis has become increasingly popular due to its benefits, such as a...
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A Stepwise Strategy Employing Automated Screening for Reversed-Phase Chromatographic Separation of Itraconazole and Its Impurities
The development of methods for the chromatographic separation of complex mixtures is often challenging, and an organized approach is required to...
