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Cryopreservable three-dimensional spheroid culture for ready-to-use systems
Three-dimensional (3D) spheroid culture has applications in many fields as spheroids closely recapitulate physiological conditions. However, spheroid...
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3D spheroid HepaRG and fluorescent biphasic tracer for CYP3A4-mediated antibiotic interaction monitoring in sepsis
Cytochrome P450 3A4 (CYP3A4) is a crucial enzyme in the metabolism of xenobiotics, particularly in drug metabolism interactions (DDIs), making it a...
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Fabrication of Cell Spheroids for 3D Cell Culture and Biomedical Applications
The significance of 3D cell culture—i.e., the maintenance of cell characteristics by mimicking the natural environment—has been increasing, and...
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Apoptotic effects of Phlomis armeniaca mediated biosynthesized silver nanoparticles in monolayer (2D) and spheroid (3D) cultures of human breast cancer cell lines
The purpose of current research was to assess the apoptotic effects of biofabrication silver nanoparticles (AgNPs) mediated by the aqueous extract of P...
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3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay
BackgroundMost high-throughput screening (HTS) systems studying the cytotoxic effect of chimeric antigen receptor (CAR) T cells on tumor cells rely...
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Optimization of Tumor Spheroid Preparation and Morphological Analysis for Drug Evaluation
Due to its similarity to in vivo conditions, tumor spheroids are actively used in research areas, such as drug screening and cell–cell interactions....
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Novel plasma-polymerized coating facilitates HeLa cell spheroid formation, exerting necroptosis via β-cyclodextrin-encapsulated resveratrol
Beta-cyclodextrins (β-CDs) comprise a pore for accommodating resveratrol (Res), thereby boosting its bioavailability. Res-incorporated β -CD (Res/CD)...
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Microfabrication methods for 3D spheroids formation and their application in biomedical engineering
Three-dimensional cell culture systems offer greater understanding of the complex human body structure than monolayer cell cultures. Spheroids, which...
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Green synthesis of glyco-CuInS2 QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging
Glyco-quantum dots (glyco-QDs) have attracted significant interest in bioimaging applications, notably in cancer imaging, because they effectively...
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An agarose-alginate microfluidic device for the study of spheroid invasion, ATRA inhibits CAFs-mediated matrix remodeling
Growing evidence demonstrates that cancer-associated fibroblasts (CAF) are responsible for tumor genesis, growth, metastasis, and treatment response....
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Disease-related biomarkers as experimental endpoints in 3D skin culture models
The success of in vitro 3D models in either recapitulating the normal tissue physiology or altered physiology or disease condition depends upon the...
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Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model
Multimodal mass spectrometry imaging (MSI) is a leading approach for investigating the molecular processes within biological samples. The parallel...
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Advances in the application of extracellular vesicles derived from three-dimensional culture of stem cells
Stem cells (SCs) have been used therapeutically for decades, yet their applications are limited by factors such as the risk of immune rejection and...
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Microfluidic 3D Cytotoxic Assay
Microfluidic-based cytotoxic assays provide high physiological relevance with the potential to replace conventional animal experiments and...
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3D stem-like spheroids-on-a-chip for personalized combinatorial drug testing in oral cancer
BackgroundFunctional drug testing (FDT) with patient-derived tumor cells in microfluidic devices is gaining popularity. However, the majority of...
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Substrate coated with autologous decellularized extracellular matrix facilitates in vitro spreading of spheroid from adipose-derived stem cells through regulating ERK1/2-MMP2/9 pathway
Adipose-derived stem cells (ADSCs) are easily available and play an important role in regenerative medicine. In recent years, Cell spheroid models...
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Stable two- and three-dimensional cholangiocyte culture systems from extrahepatic bile ducts of biliary atresia patients: use of structural and functional bile duct epithelium models for in vitro analyses
We herein report two- (2D) and three-dimensional (3D) culture methods of cholangiocytes originating from extrahepatic bile ducts of biliary atresia...
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Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flaps
In the realm of large-area trauma flap transplantation, averting ischaemic necrosis emerges as a pivotal concern. Several key mechanisms, including...
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Introduction to Animal Cell Culture
Animal cell culture has marked a significant revolution in science, allowing different fields to benefit from its use. The aim of this chapter is to...
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Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer
In pursuit of a novel effective treatment for prostate cancer, methanolic extract of Stephania glabra tubers (Sg-ME) was utilized to fabricate silver...
