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Monitoring Indian “Superfood” Moringa oleifera Lam. – species-specific PCR-fingerprint-based authentication for more consumer safety
Moringa oleifera Lam. has become one of the major new superfoods commonly available in the aisles of bio - shops and health-food sections in...
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PCR–RFLP and species-specific PCR efficiency for the identification of adulteries in meat and meat products
The traceability of meat origin has become a necessity to safeguard consumer's confidence in commercial meat products. Our study recommends a meat...
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Digital PCR: A Tool to Authenticate Herbal Products and Spices
Authentication of herbal products and spices is experiencing a resurgence using DNA-based molecular tools, mainly species-specific assays and DNA... -
Botanical Origin Authenticity Control of Pine Pollen Food Products Using Multiplex Species-Specific PCR Method
Pine pollen has been widely recognized for its comprehensive nutritional properties and health benefits, but it has also been the target of...
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Authentication of camel meat using species-specific PCR and PCR-RFLP
In India and some of the African countries, one of the unconventional meats receiving the latest attention in meat adulteration is camel meat. So,...
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Species-specific markers for Nilaparvata lugens and Sogatella furcifera (Hemiptera: Delphacidae) based on mitochondrial cytochrome oxidase I
Brown planthopper (BPH), Nilaparvata lugens (Stål) and white-backed planthopper (WBPH), Sogatella furcifera (Horváth) are the most destructive...
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Quantitative Real-Time PCR for the Detection of Allergenic Species in Foods
Food allergy is an increasing challenge to public health, with widespread global distribution. With no cure for this pathology, the food-allergic... -
Overcoming Challenges in DNA Extractions from Triphala Ingredients: A Way Forward for Optimization of Conventional and Digital PCR Assays for Molecular Authentication
Terminalia bellirica (TB), Terminalia chebula (TC), and Phyllanthus emblica (PE) fruits are renowned for their diverse therapeutic benefits,...
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Development of a tetra-primer ARMS–PCR for identification of sika and red deer and their hybrids
Accurate identification of deer-derived components is significant in food and drug authenticity. Over the years, several methods have been developed...
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Development and Evaluation of a PCR Kit for Authentication of Panax quinquefolius L. with PCR-Restriction Fragment Length Polymorphism
Panax quinquefolius L. (American ginseng) and Panax ginseng C.A. Meyer are famous herbal medicines. Accurate authentication of the species has grown...
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Establishment and Application of Multiplex PCR Scheme for Simultaneously Distinguishing Muscle Tissues of Mink, Fox, and Raccoon Dog from Conventional Livestock and Poultry
It is possible and risky for fur animal carcasses to be mixed into meat products, which is a potential danger for meat quality safety and human...
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A universal primer distinguishable PCR (UP-D-PCR) method for simultaneous identification and differentiation of bovine- and ovine/caprine-derived ingredients in ruminant feeds
Illegal addition of bovine- and ovine/caprine-derived ingredients into ruminant feeds would increase the risk of spreading transmissible spongiform...
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Optimized Design of Degenerate Primers for PCR Based on DNA or Protein Sequence Comparisons
PCR with degenerate primers can be used to identify the coding sequence of an unknown protein or to detect a genetic variant within a gene family.... -
Droplet digital PCR and real-time PCR for the sensitive and specific detection of Vibrio vulnificus based on the novel target genes
Vibrio vulnificus poses a significant risk to public health due to its high pathogenicity and mortality rates as a common seafood-borne pathogen....
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Gene Expression Quantification from Pathogenic Bacterial Biofilms by Quantitative PCR
Quantitative PCR (qPCR) is one of the most used techniques to quantify gene expression in bacterial biofilms due to its easiness, sensitivity, and... -
Development of a SYBR Green Real-Time PCR method for the detection of Coffea arabica and C. canephora using chloroplast genes
Coffea arabica (Arabica) and C. canephora (Robusta) are valuable agricultural products traded worldwide. In this study, we designed specific primer...
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DNA Isolation from Cocoa-Derived Products and Cocoa Authentication by TaqMan Real-Time PCR
Cocoa (Theobroma cacao L.) is an international commodity used as an ingredient in the manufacturing of chocolate making its authentication a key... -
A multiplex PCR method for detection of five animal species in processed meat products using novel species-specific nuclear DNA sequences
The continuous development of fast and simple new methods to identify animal-derived ingredients is very important for the authentication of meat...
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An Efficient Probe-Based Quantitative PCR Assay Targeting Human-Specific DNA in ST6GALNAC3 for the Quantification of Human Cells in Preclinical Animal Models
Precise quantification of human cells in preclinical animal models by a sensitive and specific approach is warranted. The probe-based quantitative...
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Development of real-time PCR-based markers for differentiation of Oplopanax elatus and Aralia cordata in commercial food products
Oplopanax elatus and Aralia cordata , commonly referred to as “Dureub” in Korea, are generally used as medicinal or food raw materials. Although O....