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Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of...
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Single-Molecule DNA Visualization
Direct visualization of individual DNA molecules is a powerful platform for understanding and managing genomic and biochemical phenomena in the...
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Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging
Fluorescent light-up aptamers (FLAPs) have emerged as valuable tools to visualize RNAs, but are mostly limited by their poor brightness, low...
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Single-Molecule DNA Visualization
Direct visualization of individual DNA molecules is a powerful platform for understanding and managing genomic and biochemical phenomena in the...
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Synthesis and single-molecule imaging reveal stereospecific enhancement of binding kinetics by the antitumour eEF1A antagonist SR-A3
Ternatin-family cyclic peptides inhibit protein synthesis by targeting the eukaryotic elongation factor-1α. A potentially related cytotoxic natural...
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Single-molecule fluorescence methods for protein biomarker analysis
Proteins have been considered key building blocks of life. In particular, the protein content of an organism and a cell offers significant...
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Spatiotemporally controlled generation of NTPs for single-molecule studies
Many essential processes in the cell depend on proteins that use nucleoside triphosphates (NTPs). Methods that directly monitor the often-complex...
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Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This...
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Advances in single-molecule junctions as tools for chemical and biochemical analysis
The development of miniaturized electronics has led to the design and construction of powerful experimental platforms capable of measuring electronic...
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Single-molecule imaging of glycan–lectin interactions on cells with Glyco-PAINT
Most lectins bind carbohydrate ligands with relatively low affinity, making the identification of optimal ligands challenging. Here we introduce a...
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Molecular Imaging of HER2 in Patient Tissues with Touch Prep-Quantitative Single Molecule Localization Microscopy
Biomolecules can be investigated at the nanoscale with quantitative single molecule localization microscopy (qSMLM). This technique, which achieves...
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Revisiting the Glass Treatment for Single-Molecule Analysis of ncRNA Function
Single-molecule imagingSingle molecule imaging is a powerful method for unveiling precise molecular mechanisms. Particularly, single-molecule...
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Detection of estradiol with a digital immunoassay using an anti-immunocomplex antibody and single-molecule observation of gold nanoparticles
Gold nanoparticles (AuNPs) have been widely applied to molecular sensors due to their optical properties. We previously reported a molecular...
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Single-Molecule Microscopy Methods to Study Mitochondrial Processes
Mitochondria are essential organelles of eukaryotic cells with key functions in metabolism, apoptosis, and signaling. As a result, impaired...
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Single-Molecule Fluorescence Spectroscopy of Intrinsically Disordered Proteins
The past two decades have seen a substantial leap forward in our understanding of intrinsically disordered proteins, in terms of both thermodynamics...
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Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons
Over the past years several forms of superresolution fluorescence microscopy have been developed that offer the possibility to study cellular...
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Nonlinear Optical Microcavities Towards Single-Molecule Sensing
Optical sensing techniques are of importance for detecting and understanding nanoscale objects. Optical microcavities can significantly enhance...
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Highly sensitive digital detection of SARS-CoV-2 nucleocapsid protein through single-molecule counting
Nucleocapsid protein (NP) is one of the structural proteins of SARS-CoV-2 which is stable, well-conserved, highly immunogenic, and abundantly...
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Determination of association equilibrium constant from single molecule fluorescence localization microscopy
Single molecule fluorescence localization microscopy provides molecular localization with a precision in the tens of nanometer range in the plane...
