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Single-Cell Protein Analysis Methods and Protocols
This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage...
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Mass Spectrometry Based Single Cell Proteomics
This volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book...
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Single-Cell Proteomics Analysis with Tecan Uno and SCREEN Workflow
With advances in sample preparation, small-volume liquid dispensing technologies, high-resolution MS/MS instrumentation, and data acquisition...
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Single-Cell Exogenous Gene Transfection Analysis Chip
Fast and accurate profiling of exogenous gene expressionGene expression in host cells is crucial for studying gene function in cellular and molecular...
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Deep Learning-Based Automated Analysis of NK Cell Cytotoxicity in Single Cancer Cell Arrays
The cytotoxicity assay of immune cells based on live cell imaging offers comprehensive information at the single cell-level information, but the data...
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Dielectrophoresis-Assisted Self-Digitization Chip for High-Efficiency Single-Cell Analysis
Single-cell analysis of cell phenotypic information such as surface protein expression and nucleic acid content is essential for understanding...
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Label-Free Sample Preparation for Single-Cell Proteomics
In recent years, single-cell proteomics (SCP) has become a valuable addition to other single-cell omics technologies for studying cellular...
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Bioinformatics Pipeline for Processing Single-Cell Data
Single-cell proteomics can offer valuable insights into dynamic cellular interactions, but identifying proteins at this level is challenging due to...
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Nanoliter-Scale Sample Preparation for Single-Cell Proteomic Analysis Using Glass-Oil-Air-Droplet Chip
Single-cell proteomic analyses are of fundamental importance in order to capture biological heterogeneity within complex cell systems’ heterogeneous...
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Detection of Single Non-small Cell Lung Cancer Cell Multidrug Resistance with Single-Cell Bioanalyzer
Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death in the world. Despite the development of various lung cancer...
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Discrimination of Multidrug Resistance in Cancer Cells Achieved Using Single-Cell Analysis
The biophysical signatures of single cells, such as multidrug resistance (MDR), may easily change during their various disease states. Therefore,...
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Standardized Workflow for Mass-Spectrometry-Based Single-Cell Proteomics Data Processing and Analysis Using the scp Package
Mass-spectrometry (MS)-based single-cell proteomics (SCP) explores cellular heterogeneity by focusing on the functional effectors of the...
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A Sample Preparation Procedure for Isobaric Labeling-Based Single-Cell Proteomics
Mass spectrometry-based proteomics has traditionally been limited by the amount of input material for analysis. Single-cell proteomics has emerged as...
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Single-Cell Analysis of circRNA Using ddPCR
Droplet digital polymerase chain reaction (ddPCR) is a new quantitative PCR method based on water-oil emulsion droplet technology. ddPCR enables...
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EquiCP: Targeted Single-Cell Proteomics by Mass Spectrometry with Isobaric Labeled Multiplexing
Nontargeted single-cell proteomics analysis by mass spectrometry with sample multiplexing utilizing isobaric labeling is often performed using a...
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Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis
Single cell proteomics is an emerging field of bioanalysis allowing one to capture proteome profiles of isolated single cells, which is expected to...
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Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip
Recent advancements in the profiling of proteomes at the single-cell level necessitate the development of quantitative and versatile platforms,...
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Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells
Metals have a fundamental role in microbiology, and accurate methods are needed for their identification and quantification. The inability to assess...
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Long-Read Single-Cell Sequencing Using scCOLOR-seq
Single-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its...
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Cytosolic Calcium Measurement Utilizing a Single-Cell Biochip to Study the Effect of Curcumin and Resveratrol on a Single Glioma Cell
A microfluidic method has been developed for real-time measurement of the effects of curcumin on the intracellular calcium concentration in a single...