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Metallic Vessel with Mesh Culture Surface Fabricated Using Three-dimensional Printing Engineers Tissue Culture Environment
Various culture devices have been developed as fundamental technologies for facilitating bioengineering studies. Culture devices are designed to...
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Optimization of adaptation parameters from adhesion cell culture in serum-containing media to suspension in chemically defined media by superlative box design
A new design of experiments—superlative box design (SBD), was adopted to optimize the adaptation of Chinese hamster ovary cells from adhesion culture...
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A comprehensive comparison of mixing and mass transfer in shake flasks and their relationship with MAb productivity of CHO cells
The selection of highly recombinant protein (RP)-productive Chinese hamster ovary (CHO) cell lines is widely carried out in shake flasks. It is...
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Optimization of the production process for the anticancer lead compound illudin M: improving titers in shake-flasks
BackgroundThe fungal sesquiterpenes Illudin M and S are important base molecules for the development of new anticancer agents due to their strong...
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View on a mechanistic model of Chlorella vulgaris in incubated shake flasks
AbstractKinetic growth models are a useful tool for a better understanding of microalgal cultivation and for optimizing cultivation conditions. The...
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Effect of addition of γ-poly glutamic acid on bacterial nanocellulose production under agitated culture conditions
BackgroundBacterial nanocellulose (BNC), a natural polymer material, gained significant popularity among researchers and industry. It has great...
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Production of a potential multistrain probiotic in co-culture conditions using agro-industrial by-products-based medium for fish nutrition
BackgroundProbiotics are viable microorganisms that when administered in adequate amounts confer health benefits to the host. In fish, probiotic...
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Response surface optimization of cellulase production by Aspergillus stellatus NFCCI 5299 in shake flask submerged fermentation using wheat bran
The expense of cellulase enzymes is the main barrier to the enzymatic saccharification of biomass. Numerous tactics, such as the utilizing...
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Tissue Culture of Medicinal Plants
The increasing demand for pharmaceutically important metabolites from plants has shifted the focus of research toward plant tissue culture.... -
Nitric oxide mediates red light-induced perylenequinone production in Shiraia mycelium culture
Perylenequinones (PQs) from bambusicolous Shiraia fungi serve as excellent photosensitizers for photodynamic therapy. However, the lower yield of PQ...
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Culture media selection and feeding strategy for high titer production of a lentiviral vector by stable producer clones cultivated at high cell density
The growing interest in the use of lentiviral vectors (LVs) for various applications has created a strong demand for large quantities of vectors. To...
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Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains
The concept of modular synthetic co-cultures holds considerable potential for biomanufacturing, primarily to reduce the metabolic burden of...
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Simultaneous photoautotrophic production of DHA and EPA by Tisochrysis lutea and Microchloropsis salina in co-culture
Marine microalgae have received much attention as a sustainable source of the two health beneficial omega-3-fatty acids docosahexaenoic acid (DHA,...
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Improving the Utilization of Food Waste: Conversion of Food Waste into Residual Food Dried Substance and Use of This Material as a Culture Nutrient for Microbial Production of Lactic Acid
To reduce food waste (FW) disposal costs, many Koreans now convert FW into residual food dried substances (RFDS) using a house-service food drying...
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Metabolic Burden Analysis in In Vitro Culture of Plants: A Bioprocess Strategy for Efficient Process Design
Plants serve as the source of a myriad of products owing to the therapeutic properties of their secondary metabolites. The increasing commercial... -
Lipid and Carotenoid Production by a Rhodosporidium toruloides and Tetradesmus obliquus Mixed Culture Using Primary Brewery Wastewater Supplemented with Sugarcane Molasses and Urea
In this study, Rhodosporidium toruloides and Tetradesmus obliquus were used for lipid and carotenoid production in mixed cultures using primary...
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Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media
Bacterial cellulose (BC) is a natural biodegradable, eco-friendly fiber, lying within the nanoscale range. It is reputable for its various physical...
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Effects of pullulan additive and co-culture of Aureobasidium pullulans on bacterial cellulose produced by Komagataeibacter hansenii
Bacterial cellulose (BC) exhibits a unique combination of porosity, tensile strength, reticulated crystal structure and biocompatibility useful for...
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Purification and characterization of crude fructooligosaccharides extracted from red onion (Allium cepa var. viviparum) by yeast treatment
BackgroundYeast treatment has been used for purification of fructooligosaccharides (FOSs). However, the main drawback of this approach is that yeast...
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Develo** a single-stage continuous process strategy for vitamin B12 production with Propionibacterium freudenreichii
BackgroundVitamin B 12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation...