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Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
BackgroundThe filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading...
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Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei
Trichoderma reesei ( T. reesei ) is well-known for its excellent ability to secret a large quantity of cellulase. However, unlike the endogenous...
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Secretory expression of recombinant small laccase genes in Gram-positive bacteria
BackgroundLaccases are multicopper enzymes that oxidize a wide range of aromatic and non-aromatic compounds in the presence of oxygen. The majority...
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Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii
BackgroundEukaryotes use distinct networks of biogenesis factors to synthesize, fold, monitor, traffic, and secrete proteins. During heterologous...
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The Use of Fluorescent Protein Fusions to Monitor the Unfolded Protein Response and Protein Foldase-Substrate Interactions in Plant Protoplasts
Endoplasmic reticulum (ER) stress and the resulting unfolded protein response (UPR) are critical stress response pathways in eukaryotes. To study...
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Cytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagataella phaffii
BackgroundHuman lysozyme (hLYZ) is a natural antibacterial protein with broad applications in food and pharmaceutical industries. Recombinant...
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Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction
Lipids produced by oleaginous yeasts are considered as sustainable sources for the production of biofuels and oleochemicals. The red yeast Rhodosporidi...
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Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach
The probiotic yeast Saccharomyces boulardii ( Sb ) is a promising chassis to deliver therapeutic proteins to the gut due to Sb ’s innate therapeutic...
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Intrinsically Disordered Kiwellin Protein-Like Effectors Target Plant Chloroplasts and are Extensively Present in Rust Fungi
The effector proteins produced by plant pathogens are one of the essential components of host–pathogen interaction. Despite being important, most of...
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High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris
Collagen, the highest content protein in the body, has irreplaceable biological functions, and it is widespread concerned in food, beauty, and...
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Construction and testing of Yarrowia lipolytica recombinant protein expression chassis cells based on the high-throughput screening and secretome
BackgroundIn the recombinant protein market with broad economic value, the rapid development of synthetic biology has made it necessary to construct...
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A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli
BackgroundAlthough Escherichia coli has been widely used for the expression of exogenous proteins, the secretory expression in this system is still...
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Enhanced protein secretion in reduced genome strains of Streptomyces lividans
BackgroundS. lividans TK24 is a popular host for the production of small molecules and the secretion of heterologous protein. Within its large...
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Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates
BackgroundSpecific productivity (q P ) in yeast correlates with growth, typically peaking at intermediate or maximum specific growth rates (μ)....
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Secretory production of an engineered cutinase in Bacillus subtilis for efficient biocatalytic depolymerization of polyethylene terephthalate
Polyethylene terephthalate (PET) waste has caused serious environmental pollution. Recently, PET depolymerization by enzymes with PET-depolymerizing...
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Osteoclast Methods in Protein Phosphatase Research
Osteoclasts are specialized cells that degrade bone and are essential for bone formation and maintaining bone homeostasis. Excess or deficient...
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The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii
BackgroundThe budding yeast Komagataella phaffii ( Pichia pastoris ) is widely employed to secrete proteins of academic and industrial interest. For...
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Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
Pichia pastoris (Komagataella phaffii) is widely used for industrial production of heterologous proteins due to high secretory capabilities but...
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Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae
Microbial proteins are promising substitutes for animal- and plant-based proteins. S. cerevisiae , a generally recognized as safe (GRAS)...
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Droplet-based microfluidic platform for detecting agonistic peptides that are self-secreted by yeast expressing a G-protein-coupled receptor
BackgroundSingle-cell droplet microfluidics is an important platform for high-throughput analyses and screening because it provides an independent...
